| 产品: | CCNT1 抗体 |
| 货号: | DF2288 |
| 描述: | Rabbit polyclonal antibody to CCNT1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Bovine, Horse, Sheep, Rabbit |
| 分子量: | 87 kDa; 81kD(Calculated). |
| 蛋白号: | O60563 |
| RRID: | AB_2839514 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2288, RRID:AB_2839514.
展开/折叠
CCN T1; CCNT; CCNT 1; Ccnt1; CCNT1_HUMAN; CDK9 associated C type protein; Cyc T1; Cyclin C related protein; Cyclin T; cyclin T1; Cyclin T1b; Cyclin-T; Cyclin-T1; CYCT 1; CycT1; HIVE1; Human immunodeficiency virus 1 expression; Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1; pTEFb subunit; Subunit of positive elongation transcription factor b;
抗原和靶标
- O60563 CCNT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTIDHPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCIHLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNRLKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERPFLEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSVTKSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQTSNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLNFSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISSRSGNTDKPRPPPLPSEPPPPLPPLPK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60563 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K33 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T110 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| T278 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| K342 | Sumoylation | Uniprot | |
| K376 | Methylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K380 | Acetylation | Uniprot | |
| K386 | Acetylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| K390 | Acetylation | Uniprot | |
| K390 | Methylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| K404 | Acetylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| Y418 | Phosphorylation | Uniprot | |
| Y420 | Phosphorylation | Uniprot | |
| K476 | Acetylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| K492 | Acetylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| S564 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S570 | Phosphorylation | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| Y690 | Phosphorylation | Uniprot | |
| K707 | Acetylation | Uniprot |
研究背景
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).
(Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
Nucleus.
Ubiquitously expressed.
Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with BRD4, targets chromatin binding. Interacts with JMJD6. Interacts with MDFIC. Interacts with HSF1. Interacts with HTATSF1. Interacts with AFF4. Interacts with TBX21.
(Microbial infection) Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat.
Belongs to the cyclin family. Cyclin C subfamily.
研究领域
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.