CD109 Antibody - #DF2291

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产品: CD109 抗体
货号: DF2291
描述: Rabbit polyclonal antibody to CD109
应用: WB
反应: Human, Mouse
分子量: 162 kDa; 162kD(Calculated).
蛋白号: Q6YHK3
RRID: AB_2839517

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
CD109 Antibody detects endogenous levels of total CD109.
RRID:
AB_2839517
引用格式: Affinity Biosciences Cat# DF2291, RRID:AB_2839517.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

150 KDa TGF beta 1 binding protein; 150 KDa TGF-beta-1-binding protein; 7D1; 8A3; Activated T cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD 109; CD109; CD109 antigen; CD109_HUMAN; CPAMD7; DKFZp762L1111; E123; FLJ38569; FLJ41966; Gov system alloantigens on platelets; p180; Platelet specific Gov antigen; Platelet-specific Gov antigen; r150; RP11 525G3.1;

抗原和靶标

免疫原:
Uniprot:

Q6YHK3(CD109_HUMAN) >>Visit HPA database.

基因/基因ID:
CD109(135228)
表达:
Q6YHK3 CD109_HUMAN:

Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.

描述:
Modulates negatively TGFB1 signaling in keratinocytes
序列:
MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

翻译修饰 - Q6YHK3 作为底物

Site PTM Type Enzyme
T31 Phosphorylation
N68 N-Glycosylation
Y102 Phosphorylation
N118 N-Glycosylation
K143 Ubiquitination
K169 Ubiquitination
N247 N-Glycosylation
K417 Ubiquitination
N419 N-Glycosylation
Y446 Phosphorylation
S450 Phosphorylation
K465 Ubiquitination
K478 Ubiquitination
K491 Acetylation
K491 Ubiquitination
T581 Phosphorylation
S585 Phosphorylation
Y660 Phosphorylation
T833 Phosphorylation
T840 Phosphorylation
K858 Ubiquitination
T946 Phosphorylation
K950 Ubiquitination
K1022 Ubiquitination
S1036 Phosphorylation
S1214 Phosphorylation
S1216 Phosphorylation
Y1384 Phosphorylation

研究背景

功能:

Modulates negatively TGFB1 signaling in keratinocytes.

翻译修饰:

N-glycosylated.

2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.

细胞定位:

Cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.

亚基结构:

Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.

蛋白家族:

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

文献引用

1). Aptamer-based CRISPR/Cas12a assay for the ultrasensitive detection of extracellular vesicle proteins. TALANTA (PubMed: 33076176) [IF=6.1]

Application: WB    Species: human    Sample: SUNE2 cells

Fig. 5. Evaluation of the specificity of the aptamer-CRISPR/Cas12a assay. a Schematics showing the sequence variation at different positions in CD109-apt. b CRISPR/Cas12a detection of single-strand and double-strand CD109-apt with mismatches at different positions (1–2, 3–4, 5–6, 1–4, 3–6). c Western blot analysis of the level of CD109 in SUNE2 cells after transfection with siNC and siCD109 1#, 2#, and 3#. d Histogram analysis of CD109+ EVs in the supernatant of SUNE2 cells treated with siCD109 1#, 2#, and 3# and siNC. e Histogram analysis of CD109+ EVs in the supernatants from NPC cells (HK1, HONE1, and SUNE2 cells) and normal nasopharyngeal cells (NEPC1 and NEPC2 cells). Data are based on 3 replicates; error bars = standard errors. f Histogram analysis of CD109+ EV and EGFR+ EV levels in sera from NPC patients and healthy controls. Data are based on 3 replicates; error bars = standard errors. NEPC2, NEPC2 Bmi-1.

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