(2)
(8)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2298, RRID:AB_2839524.
展开/折叠
Adapter protein CMS; AL024079; C78928; Cas ligand with multiple SH3 domains; CD2 associated protein; CD2-associated protein; CD2AP; CD2AP_HUMAN; CMS; Mesenchyme to epithelium transition protein with SH3 domains 1; METS 1; Mets1;
抗原和靶标
- Q9Y5K6 CD2AP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEFKDDSLPIKRERHGNVASLVQRISTYGLPAGGIQPHPQTKNIKKKTKKRQCKVLFEYIPQNEDELELKVGDIIDINEEVEEGWWSGTLNNKLGLFPSNFVKELEVTDDGETHEAQDDSETVLAGPTSPIPSLGNVSETASGSVTQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKPEKPLILQSLGPKTQSVEITKTDTEGKIKAKEYCRTLFAYEGTNEDELTFKEGEIIHLISKETGEAGWWRGELNGKEGVFPDNFAVQINELDKDFPKPKKPPPPAKAPAPKPELIAAEKKYFSLKPEEKDEKSTLEQKPSKPAAPQVPPKKPTPPTKASNLLRSSGTVYPKRPEKPVPPPPPIAKINGEVSSISSKFETEPVSKLKLDSEQLPLRPKSVDFDSLTVRTSKETDVVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTHSPEKILKLPKEEDSANLKPSELKKDTCYSPKPSVYLSTPSSASKANTTAFLTPLEIKAKVETDDVKKNSLDELRAQIIELLCIVEALKKDHGKELEKLRKDLEEEKTMRSNLEMEIEKLKKAVLSS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y5K6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K55 | Acetylation | Uniprot | |
| K58 | Sumoylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| T87 | Phosphorylation | Uniprot | |
| Y88 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| T231 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| T262 | Phosphorylation | Uniprot | |
| T264 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K359 | Acetylation | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K360 | Acetylation | Uniprot | |
| Y361 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| K397 | Methylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| Y409 | Phosphorylation | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| K425 | Acetylation | Uniprot | |
| K436 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| T465 | Phosphorylation | Uniprot | |
| K470 | Ubiquitination | Uniprot | |
| K495 | Methylation | Uniprot | |
| K495 | Ubiquitination | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| T512 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| T539 | Phosphorylation | Uniprot | |
| C540 | S-Nitrosylation | Uniprot | |
| Y541 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| K544 | Ubiquitination | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| Y548 | Phosphorylation | Uniprot | |
| S550 | Phosphorylation | Uniprot | |
| T551 | Phosphorylation | Uniprot | |
| S553 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| T565 | Phosphorylation | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot |
研究背景
Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation (By similarity). May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell (By similarity). May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis. Plays a role in epithelial cell junctions formation.
Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.
Cytoplasm>Cytoskeleton. Cell projection>Ruffle. Cell junction.
Note: Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles (PubMed:10339567). Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase (PubMed:15800069).
Widely expressed in fetal and adult tissues.
Homodimer. Interacts with F-actin, PKD2, NPHS1 and NPHS2. Interacts with WTIP. Interacts with DDN; interaction is direct. Interacts (via SH3 2 domain) with CBL (via phosphorylated C-terminus). Interacts with BCAR1/p130Cas (via SH3 domain). Interacts with MVB12A and ARHGAP17. Interacts with ANLN, CD2 and CBLB. Interacts with PDCD6IP and TSG101. Interacts with RIN3. Interacts directly with RET (inactive) and CBLC; upon RET activation by GDNF suggested to dissociate from RET as CBLC:CD2AP complex (Probable) Ref.29). Interacts with CGNL1 and SH3BP1; probably part of a complex at cell junctions. Interacts with CAPZA1.
The Pro-rich domain may mediate binding to SH3 domains.
Potential homodimerization is mediated by the coiled coil domain.
研究领域
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
文献引用
Application: WB Species: Mouse Sample:
Application: WB Species: Human Sample: podocytes
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