产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2336, RRID:AB_2839560.
展开/折叠
B lymphocyte serine/threonine protein kinase; B lymphocyte serine/threonine-protein kinase; BL44; GC kinase; GCK; Germinal center kinase; germinal centre kinase (GC kinase); M4K2_HUMAN; Map4k2; MAPK/ERK kinase kinase kinase 2; MEK kinase kinase 2; MEKKK 2; Mitogen activated protein kinase kinase kinase kinase 2; Mitogen-activated protein kinase kinase kinase kinase 2; Rab8 interacting protein; Rab8 interacting protein, formerly; Rab8-interacting protein; RAB8IP; RAB8IP, formerly;
抗原和靶标
Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
- Q12851 M4K2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALLRDVSLQDPRDRFELLQRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQEITILRECRHPNVVAYIGSYLRNDRLWICMEFCGGGSLQEIYHATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTASVAKRRSFIGTPYWMAPEVAAVERKGGYNELCDVWALGITAIELGELQPPLFHLHPMRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTAEKLLQHPFTTQQLPRALLTQLLDKASDPHLGTPSPEDCELETYDMFPDTIHSRGQHGPAERTPSEIQFHQVKFGAPRRKETDPLNEPWEEEWTLLGKEELSGSLLQSVQEALEERSLTIRSASEFQELDSPDDTMGTIKRAPFLGPLPTDPPAEEPLSSPPGTLPPPPSGPNSSPLLPTAWATMKQREDPERSSCHGLPPTPKVHMGACFSKVFNGCPLRIHAAVTWIHPVTRDQFLVVGAEEGIYTLNLHELHEDTLEKLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQQQVPLSIPTNRLTQRIIPRRFALSTKIPDTKGCLQCRVVRNPYTGATFLLAALPTSLLLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPEGPGCRVLFHVLPLEAGLTPDILIPPEGIPGSAQQVIQVDRDTILVSFERCVRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSLDTNEVTQEITDETRIFRVLGAHRDIILESIPTDNPEAHSNLYILTGHQSTY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12851 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| Y27 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| Y80 | Phosphorylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| Y307 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| S544 | Phosphorylation | Uniprot | |
| T574 | Phosphorylation | Uniprot | |
| T578 | Phosphorylation | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| T819 | Phosphorylation | Uniprot |
研究背景
Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion.
Polyubiquitinated through 'Lys-48'-polyubiquitin chains, allowing proteasomal turnover. Ubiquitination requires the kinase activity of MAP4K2/GCK.
Autophosphorylated in response to tumor necrosis factor (TNF), endotoxins or proinflammatory stimuli. Autophosphorylation leads to activation.
Cytoplasm. Basolateral cell membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein.
Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
Interacts with TRAF2, TRAF6, MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with RAB8A (By similarity).
The PEST domains are Pro-, Glu-, Ser-, and Thr-rich domains. Proteins with PEST domains are frequently targets of degradation by the ubiquitin proteasome.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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