| 产品: | TCEB1 抗体 |
| 货号: | DF2373 |
| 描述: | Rabbit polyclonal antibody to TCEB1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Dog, Chicken, Xenopus |
| 分子量: | 12 kDa; 12kD(Calculated). |
| 蛋白号: | Q15369 |
| RRID: | AB_2839581 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2373, RRID:AB_2839581.
展开/折叠
Elo C; EloC; ELOC_HUMAN; Elongin 15 kDa subunit; Elongin C; Elongin-C; ElonginC; RNA polymerase II transcription factor SIII subunit C; SIII; SIII p15; TCEB 1; tceb1; Transcription elongation factor B (SIII) polypeptide 1; Transcription elongation factor B polypeptide 1;
抗原和靶标
Overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3.
- Q15369 ELOC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDGEEKTYGGCEGPDAMYVKLISSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15369 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| C11 | S-Nitrosylation | Uniprot | |
| Y18 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K32 | Acetylation | Uniprot | |
| K32 | Sumoylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K72 | Acetylation | Uniprot | |
| K72 | Ubiquitination | Uniprot |
翻译修饰 - Q15369 作为激酶
| Substrate | Site | Source |
|---|---|---|
| Q02539 (HIST1H1A) | S183 | Uniprot |
研究背景
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (By similarity).
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.
Nucleus.
Overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3.
Heterotrimer of an A (ELOA, ELOA2 or ELOA3), ELOB and ELOC subunit. The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex (By similarity). Part of E3 ubiquitin ligase complexes with CUL5 or CUL2, RBX1 and a substrate adapter protein that can be either SOCS1, SOCS5, ELOA, VHL or WSB1. The elongin BC complex is part of a complex with VHL and hydroxylated HIF1A. Part of an E3 ubiquitin-protein ligase complex including ZYG11B, CUL2 and Elongin BC. Part of an E3 ubiquitin-protein ligase complex including ZER1, CUL2 and Elongin BC. Interacts with VHL. Interacts with TMF1. Interacts with SPSB1. Interacts with SPSB1. Interacts with KLHDC10; which may be an E3 ubiquitin ligase complex substrate recognition component. Interacts with NOS2 in the presence of SPSB1 or SPSB2 or SPSB4.
(Microbial infection) Substrate adapter protein can be a viral protein such as HIV Vif.
(Microbial infection) Interacts with human respiratory syncytial virus (HRSV) protein NS1.
(Microbial infection) Interacts with molluscum contagiosum virus MC132.
(Microbial infection) Interacts with herpes virus 8 virus protein LANA1.
Belongs to the SKP1 family.
研究领域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
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