TAF7L Antibody - #DF2403

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产品: TAF7L 抗体
货号: DF2403
描述: Rabbit polyclonal antibody to TAF7L
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Dog
分子量: 42 kDa; 53kD(Calculated).
蛋白号: Q5H9L4
RRID: AB_2839610

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(88%), Bovine(88%), Sheep(88%), Dog(88%)
克隆:
Polyclonal
特异性:
TAF7L Antibody detects endogenous levels of total TAF7L.
RRID:
AB_2839610
引用格式: Affinity Biosciences Cat# DF2403, RRID:AB_2839610.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cancer/testis antigen 40; CT40; FLJ23157; RNA polymerase II TBP-associated factor subunit Q; TAF2Q; TAF7 like RNA polymerase II, TATA box binding protein (TBP) associated factor; Taf7l; TAF7L_HUMAN; TATA box binding protein-associated factor, RNA polymerase II, Q; TATA box-binding protein-associated factor 50 kDa; TBP associated factor, RNA polymerase II, Q; Transcription initiation factor TFIID 50 kDa subunit; Transcription initiation factor TFIID subunit 7-like;

抗原和靶标

免疫原:
Uniprot:

Q5H9L4(TAF7L_HUMAN) >>Visit HPA database.

基因/基因ID:
TAF7L(54457)
表达:
Q5H9L4 TAF7L_HUMAN:

Testis-specific.

描述:
Probably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis.
序列:
MECPEGQLPISSENDSTPTVSTSEVTSQQEPQILVDRGSETTYESSADIAGDEGTQIPADEDTQTDADSSAQAAAQAPENFQEGKDMSESQDEVPDEVENQFILRLPLEHACTVRNLARSQSVKMKDKLKIDLLPDGRHAVVEVEDVPLAAKLVDLPCVIESLRTLDKKTFYKTADISQMLVCTADGDIHLSPEEPAASTDPNIVRKKERGREEKCVWKHGITPPLKNVRKKRFRKTQKKVPDVKEMEKSSFTEYIESPDVENEVKRLLRSDAEAVSTRWEVIAEDGTKEIESQGSIPGFLISSGMSSHKQGHTSSEYDMLREMFSDSRSNNDDDEDEDDEDEDEDEDEDEDEDKEEEEEDCSEEYLERQLQAEFIESGQYRANEGTSSIVMEIQKQIEKKEKKLHKIQNKAQRQKDLIMKVENLTLKNHFQSVLEQLELQEKQKNEKLISLQEQLQRFLKK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
88
Bovine
88
Sheep
88
Dog
88
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q5H9L4 作为底物

Site PTM Type Enzyme
T184 Phosphorylation
T223 Phosphorylation
S258 Phosphorylation

研究背景

功能:

Probably functions as a spermatogenesis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis (By similarity).

细胞定位:

Nucleus. Cytoplasm.
Note: Cytoplasmic in spermatogonia and early spermatocytes (preleptotene, leptotene, and zygotene); translocates into the nuclei of pachytene spermatocytes and round spermatids.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Testis-specific.

亚基结构:

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TAF7L may replace TAF7 in a spermatogenesis-specific form of TFIID. Interacts with TBP; the interaction occurs in a sub-population of cells (pachytene and haploid round spermatids) and is developmentally regulated through differential intracellular localization of the two proteins. Interacts with TAF1 (By similarity).

蛋白家族:

Belongs to the TAF7 family.

研究领域

· Genetic Information Processing > Transcription > Basal transcription factors.

文献引用

1). Genetic characterization of a missense mutation in the X-linked TAF7L gene identified in an oligozoospermic man. BIOLOGY OF REPRODUCTION, 2022 (PubMed: 35554494) [IF=3.6]

Application: WB    Species: Mouse    Sample:

Figure 2 Generation of Taf7lD144G knockin mice. (A) Schematic of mouse Taf7l gene structure, targeting vector, and various alleles. Taf7l consists of 13 exons. Only the first nine exons are shown in rectangles. Exons 10 to 13 are not shown. The asterisk in Exon 4 denotes the point mutation. PGK-Neo encodes a neomycin selection marker. HyTK enables positive selection with hygromycin (not used) and negative selection with ganciclovir. pOG231 expresses Cre recombinase. (B) Genotyping of Taf7l alleles. (C) Sequencing of the wild type and Taf7lD144G alleles. (D) qRT-PCR analysis of the Taf7l transcript in wild type and Taf7lD144G/Y testes from 3-month-old mice. n.s.: nonsignificant, by Student’s t-test. (E) Western blot analysis of TAF7L in 3-month-old wild type and Taf7lD144G/Y testes. GAPDH serves as a loading control.

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