Cyclin A Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	Cyclin A 抗体
货号:	AF0142
描述:	Rabbit polyclonal antibody to Cyclin A
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat, Monkey
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量:	53kDa; 52kD,49kD(Calculated).
蛋白号:	P78396 \| P20248
RRID:	AB_2833324

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat,Monkey

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

克隆:

Polyclonal

特异性:

Cyclin A Antibody detects endogenous levels of total Cyclin A.

RRID:

AB_2833324
引用格式: Affinity Biosciences Cat# AF0142, RRID:AB_2833324.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

CCN A1; CCNA 1; Ccna1; CCNA1_HUMAN; Cyclin-A1; CyclinA1; G2/mitotic specific cyclin A1; MGC132235; MGC159139; CCN1; CCNA; Ccna2; CCNA2_HUMAN; Cyclin A2; Cyclin-A; Cyclin-A2;

抗原和靶标

免疫原:

Uniprot:

P78396(CCNA1_HUMAN) >>Visit HPA database.

P20248(CCNA2_HUMAN) >>Visit HPA database.

基因/基因ID:

CCNA2(890) | CCNA1(8900)

表达:

P78396 CCNA1_HUMAN:

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

描述:

CCNA1 May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Belongs to the cyclin family；CCNA2 a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle.

序列:

P78396 CCNA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

METGFPAIMYPGSFIGGWGEEYLSWEGPGLPDFVFQQQPVESEAMHCSNPKSGVVLATVARGPDACQILTRAPLGQDPPQRTVLGLLTANGQYRRTCGQGITRIRCYSGSENAFPPAGKKALPDCGVQEPPKQGFDIYMDELEQGDRDSCSVREGMAFEDVYEVDTGTLKSDLHFLLDFNTVSPMLVDSSLLSQSEDISSLGTDVINVTEYAEEIYQYLREAEIRHRPKAHYMKKQPDITEGMRTILVDWLVEVGEEYKLRAETLYLAVNFLDRFLSCMSVLRGKLQLVGTAAMLLASKYEEIYPPEVDEFVYITDDTYTKRQLLKMEHLLLKVLAFDLTVPTTNQFLLQYLRRQGVCVRTENLAKYVAELSLLEADPFLKYLPSLIAAAAFCLANYTVNKHFWPETLAAFTGYSLSEIVPCLSELHKAYLDIPHRPQQAIREKYKASKYLCVSLMEPPAVLLLQ

P20248 CCNA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P78396/P20248 作为底物

P78396
P20248

Site	PTM Type	Source
	Ubiquitination	Uniprot
T168	Phosphorylation	Uniprot
S280	Phosphorylation	Uniprot

Site	PTM Type	Enzyme	Source
	Ubiquitination		Uniprot
M1	Acetylation		Uniprot
S5	Phosphorylation		Uniprot
K37	Ubiquitination		Uniprot
K54	Acetylation		Uniprot
K54	Ubiquitination		Uniprot
S55	Phosphorylation		Uniprot
K68	Acetylation		Uniprot
K76	Acetylation		Uniprot
K95	Acetylation		Uniprot
K95	Ubiquitination		Uniprot
K112	Acetylation		Uniprot
Y146	Phosphorylation		Uniprot
S151	Phosphorylation		Uniprot
S154	Phosphorylation	P24941 (CDK2)	Uniprot
K192	Ubiquitination		Uniprot
S209	Phosphorylation		Uniprot
Y225	Phosphorylation		Uniprot
S244	Phosphorylation		Uniprot
T380	Phosphorylation		Uniprot
K388	Ubiquitination		Uniprot
K400	Ubiquitination		Uniprot
K417	Ubiquitination		Uniprot

研究背景

P78396_CCNA1_HUMAN
P20248_CCNA2_HUMAN

功能:

May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.

翻译修饰:

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

细胞定位:

Nucleus.

组织特异性:

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

亚基结构:

Interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Does not bind CDK4 and CDK5 (in vitro). The cyclin A1-CDK2 complex interacts with transcription factor E2F-1 and RB proteins. Found in a complex with CDK2, CABLES1 and CCNE1 (By similarity). Interacts with INCA1. Interacts with KLHDC9.

蛋白家族:

Belongs to the cyclin family. Cyclin AB subfamily.

功能:

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.

翻译修饰:

细胞定位:

Nucleus. Cytoplasm.
Note: Exclusively nuclear during interphase (PubMed:1312467). Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).

亚基结构:

Interacts with the CDK1 and CDK2 protein kinases to form serine/threonine kinase holoenzyme complexes. Interacts with CDK1 (hyperphosphorylated form in G1 and underphosphorylated forms in S and G2). Interacts with CDK2; the interaction increases from G1 to G2. Interacts (associated with CDK2 but not with CDK1) with SCAPER; regulates the activity of CCNA2/CDK2 by transiently maintaining CCNA2 in the cytoplasm. Forms a ternary complex with CDK2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with INCA1.

(Microbial infection) Interacts with human cytomegalovirus protein UL32.

蛋白家族:

Belongs to the cyclin family. Cyclin AB subfamily.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

文献引用

1). TCA-phospholipid-glycolysis targeted triple therapy effectively suppresses ATP production and tumor growth in glioblastoma. Theranostics, 2023 (PubMed: 36276638) [IF=12.4]

Application: WB Species: Human Sample: TBD0220 and U87MG cells

Figure 3 Reduction in ATP production hinders cell proliferation and contributes to G1/S arrest in GBM. (A-B) The relative viability of TBD0220 (A) and U87MG (B) cells was measured using a CCK-8 kit (n = 3). (C-D) Colony formation assay to detect GBM cell growth (D), and the quantification of colony numbers (C) (n = 3). (E) Cell cycle analysis using flow cytometry after incubation with different treatments like EPIC (20 µM), AA (25 µM), or EPIC (20 µM) + AA (25 µM) for 48 h, and the results are plotted as a histogram (n = 3) (F) Representative western blotting showing the expression of p21 and Rb and their downstream targets. The results were normalized to Tubulin with the control group as 1. Protein expression was quantified by ImageJ. (G) Representative confocal images of CDK6 after the treatment with AA (25 µM), EPIC (20 µM), or EPIC (20 µM) + AA (25 µM) for 48 h (n = 6). (H) The quantitative analysis of fluorescence images of CDK6 (n = 6). All data are shown as the mean values ± SD, and p values are based on one-way or two-way ANOVA. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Scale bar = 20 μm.

2). Combination LSD1 and HOTAIR-EZH2 inhibition disrupts cell cycle processes and induces apoptosis in glioblastoma cells. PHARMACOLOGICAL RESEARCH, 2021 (PubMed: 34246782) [IF=9.3]

3). Quercetin induces MGMT+ glioblastoma cells apoptosis via dual inhibition of Wnt3a/β-Catenin and Akt/NF-κB signaling pathways. Phytomedicine, 2023 (PubMed: 37451151) [IF=7.9]

4). Isobavachalcone, a natural sirtuin 2 inhibitor, exhibits anti-triple-negative breast cancer efficacy in vitro and in vivo. Phytotherapy research : PTR, 2024 (PubMed: 38349045) [IF=7.2]

5). Trans-anethole ameliorates LPS-induced inflammation via suppression of TLR4/NF-κB pathway in IEC-6 cells. International Immunopharmacology, 2022 (PubMed: 35617845) [IF=5.6]

6). The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer. INTERNATIONAL JOURNAL OF ONCOLOGY, 2017 (PubMed: 29048616) [IF=5.2]

Application: WB Species: human Sample:

7). High Iodine Induces the Proliferation of Papillary and Anaplastic Thyroid Cancer Cells via AKT/Wee1/CDK1 Axis. Frontiers in Oncology, 2021 (PubMed: 33796458) [IF=4.7]

8). CD38 enhances the proliferation and inhibits the apoptosis of cervical cancer cells by affecting the mitochondria functions. MOLECULAR CARCINOGENESIS, 2017 (PubMed: 28544069) [IF=4.6]

Application: WB Species: human Sample:

Western blot analysis indicated that CD38 correlated with P53/P21/cyclinD1 pathway

9). Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells. PLoS One, 2018 (PubMed: 29684087) [IF=3.7]

Application: WB Species: mouse Sample: A375 cells

Fig 5. |Western blot results. (a) The levels of cyclin A, cyclin B, cyclin D1, and CDK2 were reduced in A375 cells transfected with OVOS2-shRNA

10). IDH1 gene mutation activates Smad signaling molecules to regulate the expression levels of cell cycle and biological rhythm genes in human glioma U87‑MG cells. Molecular Medicine Reports, 2021 (PubMed: 33760141) [IF=3.4]

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Cyclin A Antibody - #AF0142

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