产品: | XRN1 抗体 |
货号: | DF2433 |
描述: | Rabbit polyclonal antibody to XRN1 |
应用: | WB IF/ICC |
反应: | Human, Mouse |
预测: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 194 kDa; 194kD(Calculated). |
蛋白号: | Q8IZH2 |
RRID: | AB_2839639 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2433, RRID:AB_2839639.
展开/折叠
5'-3' exoribonuclease 1; Dhm2; mXRN1; SEP1; Strand-exchange protein 1 homolog;
抗原和靶标
Expressed in heart, brain, pancreas, spleen, testis, osteogenic sarcoma (OGS) biopsy and primary cell lines.
- Q8IZH2 XRN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREHFSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLSDIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSPIIEYYPPDFKTDLNGKQQEWEAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFIYPSPWPEKFPAIERCCTRYKIISLDAWRVDINKNKITRIDQKALYFCGFPTLKHIRHKFFLKKSGVQVFQQSSRGENMMLEILVDAESDELTVENVASSVLGKSVFVNWPHLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKVVHLGDKEQSNWAKEVQGISEHYLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFDSRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDALIQNQHKYSIKYNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKKVGSEWMYSSAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLKGHPVSTLSRSSCDLQILDAAIVEKIEEEVEKCKQRKNNKKVRVTVKPHLLYRPLEQQHGVIPDRDAEFCLFDRVVNVRENFSVPVGLRGTIIGIKGANREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVKPQPAVHQHSSSSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVLPQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIENFLASLNISKENEVQSSHHGEPPSEEHLSPQSFAMGTRMLKEILKIDGSNTVDHKNEIKQIANEIPVSSNRRDEYGLPSQPKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVSTPVTELSRICSLVGMPQPDFSFLRMPQTMTVCQVKLSNGLLVHGPQCHSENEAKEKAALFALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPTGWDHYGSNYALGAANIMPSSSHLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKKRVANKKNFENKEAQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHHKSTPISSSRRKSRKLAVNFGVSKPSE
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8IZH2 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K79 | Ubiquitination | Uniprot | |
K118 | Ubiquitination | Uniprot | |
K268 | Acetylation | Uniprot | |
T270 | Phosphorylation | Uniprot | |
Y273 | Phosphorylation | Uniprot | |
K338 | Ubiquitination | Uniprot | |
K342 | Ubiquitination | Uniprot | |
K364 | Ubiquitination | Uniprot | |
K368 | Ubiquitination | Uniprot | |
Y384 | Phosphorylation | Uniprot | |
K391 | Ubiquitination | Uniprot | |
S396 | Phosphorylation | Uniprot | |
K413 | Ubiquitination | Uniprot | |
K572 | Ubiquitination | Uniprot | |
S584 | Phosphorylation | Uniprot | |
K586 | Ubiquitination | Uniprot | |
K618 | Ubiquitination | Uniprot | |
K652 | Ubiquitination | Uniprot | |
K673 | Ubiquitination | Uniprot | |
K736 | Ubiquitination | Uniprot | |
K747 | Ubiquitination | Uniprot | |
T753 | Phosphorylation | Uniprot | |
K758 | Ubiquitination | Uniprot | |
K765 | Ubiquitination | Uniprot | |
K772 | Ubiquitination | Uniprot | |
Y797 | Phosphorylation | Uniprot | |
T802 | Phosphorylation | Uniprot | |
K805 | Ubiquitination | Uniprot | |
K818 | Ubiquitination | Uniprot | |
Y829 | Phosphorylation | Uniprot | |
S844 | Phosphorylation | Uniprot | |
K847 | Ubiquitination | Uniprot | |
K910 | Ubiquitination | Uniprot | |
R939 | Methylation | Uniprot | |
K950 | Ubiquitination | Uniprot | |
K971 | Ubiquitination | Uniprot | |
Y990 | Phosphorylation | Uniprot | |
K1035 | Ubiquitination | Uniprot | |
K1130 | Ubiquitination | Uniprot | |
S1173 | Phosphorylation | Uniprot | |
S1176 | Phosphorylation | Uniprot | |
K1228 | Ubiquitination | Uniprot | |
K1245 | Ubiquitination | Uniprot | |
Y1248 | Phosphorylation | Uniprot | |
K1256 | Ubiquitination | Uniprot | |
K1272 | Ubiquitination | Uniprot | |
S1279 | Phosphorylation | Uniprot | |
K1281 | Ubiquitination | Uniprot | |
Y1282 | Phosphorylation | Uniprot | |
S1299 | Phosphorylation | Uniprot | |
K1301 | Ubiquitination | Uniprot | |
S1306 | Phosphorylation | Uniprot | |
K1308 | Ubiquitination | Uniprot | |
S1348 | Phosphorylation | Uniprot | |
K1364 | Acetylation | Uniprot | |
K1364 | Ubiquitination | Uniprot | |
K1374 | Ubiquitination | Uniprot | |
K1378 | Ubiquitination | Uniprot | |
Y1394 | Phosphorylation | Uniprot | |
S1398 | Phosphorylation | Uniprot | |
K1401 | Ubiquitination | Uniprot | |
K1412 | Acetylation | Uniprot | |
Y1419 | Phosphorylation | Uniprot | |
S1451 | Phosphorylation | Uniprot | |
K1621 | Ubiquitination | Uniprot | |
T1629 | Phosphorylation | Uniprot | |
T1633 | Phosphorylation | Uniprot | |
S1638 | Phosphorylation | Uniprot | |
S1645 | Phosphorylation | Uniprot | |
S1653 | Phosphorylation | Uniprot | |
S1692 | Phosphorylation | Uniprot |
研究背景
Major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'-processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA (By similarity). Plays a role in replication-dependent histone mRNA degradation. May act as a tumor suppressor protein in osteogenic sarcoma (OGS).
Cytoplasm.
Note: Discrete foci at the inner surface of the cell membrane.
Expressed in heart, brain, pancreas, spleen, testis, osteogenic sarcoma (OGS) biopsy and primary cell lines.
Found in a mRNP complex with UPF1, UPF2, UPF3B and XRN1. Associates with alpha and beta tubulins (By similarity). Interacts with DIS3L2. Interacts with ZC3HAV1 in an RNA-dependent manner. Interacts with ZFP36L1. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner. Interacts with YTHDC2 (via ANK repeats).
Belongs to the 5'-3' exonuclease family.
研究领域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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