产品: EBP2 抗体
货号: DF2454
描述: Rabbit polyclonal antibody to EBP2
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 35 kDa; 35kD(Calculated).
蛋白号: Q99848
RRID: AB_2839660

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%), Chicken(82%), Xenopus(90%)
克隆:
Polyclonal
特异性:
EBP2 Antibody detects endogenous levels of total EBP2.
RRID:
AB_2839660
引用格式: Affinity Biosciences Cat# DF2454, RRID:AB_2839660.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EBNA1-binding protein 2; ebna1bp2; EBP2; EBP2_HUMAN; NOBP; nuclear FGF3 binding protein; Nucleolar protein p40; P40; Probable rRNA processing protein EBP2; Probable rRNA-processing protein ebp2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q99848 EBP2_HUMAN:

Ubiquitous.

描述:
Required for the processing of the 27S pre-rRNA.
序列:
MDTPPLSDSESESDESLVTDRELQDAFSRGLLKPGLNVVLEGPKKAVNDVNGLKQCLAEFKRDLEWVERLDVTLGPVPEIGGSEAPAPQNKDQKAVDPEDDFQREMSFYRQAQAAVLAVLPRLHQLKVPTKRPTDYFAEMAKSDLQMQKIRQKLQTKQAAMERSEKAKQLRALRKYGKKVQTEVLQKRQQEKAHMMNAIKKYQKGFSDKLDFLEGDQKPLAQRKKAGAKGQQMRKGPSAKRRYKNQKFGFGGKKKGSKWNTRESYDDVSSFRAKTAHGRGLKRPGKKGSNKRPGKRTREKMKNRTH

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Xenopus
90
Chicken
82
Zebrafish
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99848 作为底物

Site PTM Type Enzyme
M1 Acetylation
T3 Phosphorylation
S7 Phosphorylation
S9 Phosphorylation
S11 Phosphorylation
S13 Phosphorylation
S16 Phosphorylation
T19 Phosphorylation
K33 Ubiquitination
K54 Ubiquitination
K61 Acetylation
T73 Phosphorylation
K94 Ubiquitination
K131 Ubiquitination
Y136 Phosphorylation
S143 Phosphorylation
K149 Ubiquitination
K157 Ubiquitination
K166 Acetylation
K168 Acetylation
K175 Acetylation
K179 Acetylation
K179 Ubiquitination
T182 Phosphorylation O14757 (CHEK1)
K187 Acetylation
K187 Ubiquitination
K192 Ubiquitination
K201 Ubiquitination
S207 Phosphorylation
K209 Acetylation
K209 Ubiquitination
K218 Sumoylation
K218 Ubiquitination
K224 Methylation
K235 Acetylation
K247 Ubiquitination
K253 Acetylation
K258 Ubiquitination
S264 Phosphorylation
Y265 Phosphorylation
S270 Phosphorylation

研究背景

功能:

Required for the processing of the 27S pre-rRNA.

细胞定位:

Nucleus>Nucleolus.
Note: Associated with the nucleolus in an RNA-dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Specifically interacts with EBV EBNA1. The EBNA1-EBP2 interaction is important for the stable segregation of EBV episomes during cell division. Interacts with WDR46.

蛋白家族:

Belongs to the EBP2 family.

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