IL15 Antibody - #DF2521

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产品: IL15 抗体
货号: DF2521
描述: Rabbit polyclonal antibody to IL15
应用: WB IHC
反应: Human, Mouse
预测: Horse, Rabbit
分子量: 18 kDa; 18kD(Calculated).
蛋白号: P40933
RRID: AB_2839727

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Horse(100%), Rabbit(88%)
克隆:
Polyclonal
特异性:
IL15 Antibody detects endogenous levels of total IL15.
RRID:
AB_2839727
引用格式: Affinity Biosciences Cat# DF2521, RRID:AB_2839727.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IL 15; IL-15; IL15; IL15_HUMAN; Interleukin 15; Interleukin-15; Interleukin15; MGC9721;

抗原和靶标

免疫原:
Uniprot:

P40933(IL15_HUMAN) >>Visit HPA database.

基因/基因ID:
IL15(3600)
表达:
P40933 IL15_HUMAN:

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.

描述:
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
序列:
MRISKPHLRSISIQCYLCLLLNSHFLTEAGIHVFILGCFSAGLPKTEANWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Rabbit
88
Dog
75
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P40933 作为底物

Site PTM Type Enzyme
T46 Phosphorylation
S55 Phosphorylation

研究背景

功能:

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.

细胞定位:

Secreted.

Cytoplasm. Nucleus.
Note: IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.

蛋白家族:

Belongs to the IL-15/IL-21 family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

文献引用

1). IL-15 Participates in the Pathogenesis of Polycystic Ovary Syndrome by Affecting the Activity of Granulosa Cells. Frontiers in Endocrinology, 2022 (PubMed: 35250857) [IF=5.2]

Application: IF/ICC    Species: Mice    Sample: ovarian tissues

Figure 3 Higher level of IL-15 in PCOS model mice. (A, B). ELISA detection of IL-15 in the serum and ovarian tissue homogenate of two groups of mice (n=3 per group, P=0.2146, 0.0026; R=0.3044, 0.9179 respectively); (C) PCR detection of IL-15 in ovarian tissues in the two groups (CON n=5, PCOS n=6, P=0.0032, R=0.5984); (D) PCR detection of IL-15 in adipose tissue in the two groups (n=6 per group, P=0.0103, R=0.5819); (E) IHC analysis of IL-15 in the ovaries of two groups of mice. The brown part represents the part of IL-15 expression. Bar=50um, 40X. Images are representative of three independent experiments with similar results (P=0.0055, R=0.5541); (F), Blue shows the nucleus (DAPI), green fluorescence shows ovarian granulosa cells marker FSHR (TSA 520, excitation and emission wavelengths similar to FITC), red shows IL-15(TSA 650, excitation and emission wavelengths similar to AF610). Bar=250um. *P < 0.05, **P < 0.01 versus the control.

Application: IHC    Species: Mice    Sample: ovarian tissues

Figure 3 Higher level of IL-15 in PCOS model mice. (A, B). ELISA detection of IL-15 in the serum and ovarian tissue homogenate of two groups of mice (n=3 per group, P=0.2146, 0.0026; R=0.3044, 0.9179 respectively); (C) PCR detection of IL-15 in ovarian tissues in the two groups (CON n=5, PCOS n=6, P=0.0032, R=0.5984); (D) PCR detection of IL-15 in adipose tissue in the two groups (n=6 per group, P=0.0103, R=0.5819); (E) IHC analysis of IL-15 in the ovaries of two groups of mice. The brown part represents the part of IL-15 expression. Bar=50um, 40X. Images are representative of three independent experiments with similar results (P=0.0055, R=0.5541); (F), Blue shows the nucleus (DAPI), green fluorescence shows ovarian granulosa cells marker FSHR (TSA 520, excitation and emission wavelengths similar to FITC), red shows IL-15(TSA 650, excitation and emission wavelengths similar to AF610). Bar=250um. *P < 0.05, **P < 0.01 versus the control.

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