ITGA2 Antibody - #DF2540

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产品: ITGA2 抗体
货号: DF2540
描述: Rabbit polyclonal antibody to ITGA2
应用: WB IHC IF/ICC
反应: Human, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 150 kDa; 129kD(Calculated).
蛋白号: P17301
RRID: AB_2839746

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Rat
预测:
Pig(90%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(82%), Dog(91%)
克隆:
Polyclonal
特异性:
ITGA2 Antibody detects endogenous levels of total ITGA2.
RRID:
AB_2839746
引用格式: Affinity Biosciences Cat# DF2540, RRID:AB_2839746.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BR; CD 49b; CD49 antigen like family member B; CD49 antigen-like family member B; CD49b; CD49b antigen; Collagen receptor; DX5; Glycoprotein Ia deficiency included; GP Ia; GP Ia deficiency, included; GPIa; HPA 5 included; HPA5 included; Human platelet alloantigen system 5; Integrin alpha 2; Integrin alpha-2; Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor); ITA2_HUMAN; ITGA2; Platelet alloantigen Br(a), included; Platelet antigen Br; Platelet glycoprotein GPIa; Platelet glycoprotein Ia; Platelet glycoprotein Ia/IIa; Platelet membrane glycoprotein Ia; Platelet receptor for collagen, deficiency of, included; Very late activation protein 2 receptor alpha 2 subunit; VLA 2 alpha chain; VLA 2; VLA 2 subunit alpha; VLA-2 subunit alpha; VLA2; VLA2 receptor alpha 2 subunit; VLAA2;

抗原和靶标

免疫原:
Uniprot:

P17301(ITA2_HUMAN) >>Visit HPA database.

基因/基因ID:
ITGA2(3673)
描述:
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
序列:
MGPERTGAAPLPLLLVLALSQGILNCCLAYNVGLPEAKIFSGPSSEQFGYAVQQFINPKGNWLLVGSPWSGFPENRMGDVYKCPVDLSTATCEKLNLQTSTSIPNVTEMKTNMSLGLILTRNMGTGGFLTCGPLWAQQCGNQYYTTGVCSDISPDFQLSASFSPATQPCPSLIDVVVVCDESNSIYPWDAVKNFLEKFVQGLDIGPTKTQVGLIQYANNPRVVFNLNTYKTKEEMIVATSQTSQYGGDLTNTFGAIQYARKYAYSAASGGRRSATKVMVVVTDGESHDGSMLKAVIDQCNHDNILRFGIAVLGYLNRNALDTKNLIKEIKAIASIPTERYFFNVSDEAALLEKAGTLGEQIFSIEGTVQGGDNFQMEMSQVGFSADYSSQNDILMLGAVGAFGWSGTIVQKTSHGHLIFPKQAFDQILQDRNHSSYLGYSVAAISTGESTHFVAGAPRANYTGQIVLYSVNENGNITVIQAHRGDQIGSYFGSVLCSVDVDKDTITDVLLVGAPMYMSDLKKEEGRVYLFTIKKGILGQHQFLEGPEGIENTRFGSAIAALSDINMDGFNDVIVGSPLENQNSGAVYIYNGHQGTIRTKYSQKILGSDGAFRSHLQYFGRSLDGYGDLNGDSITDVSIGAFGQVVQLWSQSIADVAIEASFTPEKITLVNKNAQIILKLCFSAKFRPTKQNNQVAIVYNITLDADGFSSRVTSRGLFKENNERCLQKNMVVNQAQSCPEHIIYIQEPSDVVNSLDLRVDISLENPGTSPALEAYSETAKVFSIPFHKDCGEDGLCISDLVLDVRQIPAAQEQPFIVSNQNKRLTFSVTLKNKRESAYNTGIVVDFSENLFFASFSLPVDGTEVTCQVAASQKSVACDVGYPALKREQQVTFTINFDFNLQNLQNQASLSFQALSESQEENKADNLVNLKIPLLYDAEIHLTRSTNINFYEISSDGNVPSIVHSFEDVGPKFIFSLKVTTGSVPVSMATVIIHIPQYTKEKNPLMYLTGVQTDKAGDISCNADINPLKIGQTSSSVSFKSENFRHTKELNCRTASCSNVTCWLKDVHMKGEYFVNVTTRIWNGTFASSTFQTVQLTAAAEINTYNPEIYVIEDNTVTIPLMIMKPDEKAEVPTGVIIGSIIAGILLLLALVAILWKLGFFKRKYEKMTKNPDEIDETTELSS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
91
Dog
91
Pig
90
Bovine
82
Sheep
82
Rabbit
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P17301 作为底物

Site PTM Type Enzyme
Y50 Phosphorylation
T101 Phosphorylation
Y262 Phosphorylation
Y264 Phosphorylation
T282 Phosphorylation
N343 N-Glycosylation
T412 Phosphorylation
S601 Phosphorylation
R612 Methylation
S613 Phosphorylation
S621 Phosphorylation
K671 Ubiquitination
T712 Phosphorylation
S713 Phosphorylation
S768 Phosphorylation
Y774 Phosphorylation
K821 Ubiquitination
T978 Phosphorylation
Y1005 Phosphorylation
Y1071 Phosphorylation
T1076 Phosphorylation
K1168 Ubiquitination
T1177 Phosphorylation
S1180 Phosphorylation
S1181 Phosphorylation

研究背景

功能:

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A.

(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.

细胞定位:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer of an alpha and a beta subunit. Alpha-2 associates with beta-1. Interacts with HPS5 and RAB21.

(Microbial infection) Integrin ITGA2:ITGB1 interacts (via ITAG2 I-domain) with rotavirus A VP4 protein.

(Microbial infection) Integrin ITGA2:ITGB1 interacts with human echoviruses 1 and 8 capsid proteins.

蛋白家族:

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Belongs to the integrin alpha chain family.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

文献引用

1). Osthole inhibits the migration and invasion of highly metastatic breast cancer cells by suppressing ITGα3/ITGβ5 signaling. Acta Pharmacologica Sinica, 2021 (PubMed: 34426644) [IF=8.2]

Application: WB    Species: Human    Sample: breast cancer cells

Fig. 3 Identification of candidate target genes of osthole in highly bone-metastatic breast cancer cells by RNA sequencing. a KEGG pathways enrichment analysis of differentially expressed genes (DEGs) in MDA-MB-231BO cells after osthole treatment. b Heatmap of DEGs between MDA-MB-231BO cells and MDA-MB-231BO + osthole cells (|log2 Fold Change| > 1, P 
2). MicroRNA-145 overexpression attenuates apoptosis and increases matrix synthesis in nucleus pulposus cells. LIFE SCIENCES, 2019 (PubMed: 30797016) [IF=6.1]
3). Effects of Cinobufagin on the Proliferation, Migration, and Invasion of H1299 Lung Cancer Cells. Chemistry & Biodiversity, 2023 (PubMed: 36522286) [IF=2.9]

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