KIF23 Antibody - #DF2573

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产品: KIF23 抗体
货号: DF2573
描述: Rabbit polyclonal antibody to KIF23
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 89 kDa; 110kD(Calculated).
蛋白号: Q02241
RRID: AB_2839779

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(85%), Bovine(85%), Horse(85%), Sheep(85%), Dog(85%), Chicken(83%), Xenopus(100%)
克隆:
Polyclonal
特异性:
KIF23 Antibody detects endogenous levels of total KIF23.
RRID:
AB_2839779
引用格式: Affinity Biosciences Cat# DF2573, RRID:AB_2839779.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CHO 1; CHO1; KIF 23; KIF23; KIF23_HUMAN; Kinesin family member 23; Kinesin like 5; Kinesin like protein 5; Kinesin like protein KIF 23; Kinesin like protein KIF23; Kinesin-like protein 5; Kinesin-like protein KIF23; KNS L5; KNSL 5; KNSL5; Mitotic kinesin like 1; Mitotic kinesin like protein 1; Mitotic kinesin-like protein 1; MKLP 1;

抗原和靶标

免疫原:
Uniprot:

Q02241(KIF23_HUMAN) >>Visit HPA database.

基因/基因ID:
KIF23(9493)
描述:
Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
序列:
MKSARAKTPRKPTVKKGSQTNLKDPVGVYCRVRPLGFPDQECCIEVINNTTVQLHTPEGYRLNRNGDYKETQYSFKQVFGTHTTQKELFDVVANPLVNDLIHGKNGLLFTYGVTGSGKTHTMTGSPGEGGLLPRCLDMIFNSIGSFQAKRYVFKSNDRNSMDIQCEVDALLERQKREAMPNPKTSSSKRQVDPEFADMITVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVPFDPIKPKPPQSKLLREDKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFNIKLVQAPLDADGDNVLQEKEQITISQLSLVDLAGSERTNRTRAEGNRLREAGNINQSLMTLRTCMDVLRENQMYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEENLQVMRFAEVTQEVEVARPVDKAICGLTPGRRYRNQPRGPVGNEPLVTDVVLQSFPPLPSCEILDINDEQTLPRLIEALEKRHNLRQMMIDEFNKQSNAFKALLQEFDNAVLSKENHMQGKLNEKEKMISGQKLEIERLEKKNKTLEYKIEILEKTTTIYEEDKRNLQQELETQNQKLQRQFSDKRRLEARLQGMVTETTMKWEKECERRVAAKQLEMQNKLWVKDEKLKQLKAIVTEPKTEKPERPSRERDREKVTQRSVSPSPVPLSSNYIAQISNGQQLMSQPQLHRRSNSCSSISVASCISEWEQKIPTYNTPLKVTSIARRRQQEPGQSKTCIVSDRRRGMYWTEGREVVPTFRNEIEIEEDHCGRLLFQPDQNAPPIRLRHRRSRSAGDRWVDHKPASNMQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIETKLIKGDIYKTRGGGQSVQFTDIETLKQESPNGSRKRRSSTVAPAQPDGAESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Xenopus
100
Pig
85
Horse
85
Bovine
85
Sheep
85
Dog
85
Chicken
83
Rabbit
78
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q02241 作为底物

Site PTM Type Enzyme
T8 Phosphorylation
T13 Phosphorylation
K16 Ubiquitination
S18 Phosphorylation
T20 Phosphorylation
K23 Ubiquitination
Y29 Phosphorylation
T56 Phosphorylation
K86 Ubiquitination
Y111 Phosphorylation
S116 Phosphorylation
S125 Phosphorylation
K149 Ubiquitination
K154 Ubiquitination
S155 Phosphorylation
S160 Phosphorylation
K183 Acetylation
K188 Acetylation
S298 Phosphorylation
S303 Phosphorylation
K325 Ubiquitination
K384 Ubiquitination
Y388 Phosphorylation
K406 Ubiquitination
K444 Ubiquitination
T450 Phosphorylation
K503 Ubiquitination
K517 Ubiquitination
K523 Ubiquitination
K536 Ubiquitination
K543 Ubiquitination
S552 Phosphorylation
K555 Ubiquitination
K566 Ubiquitination
K571 Ubiquitination
K577 Ubiquitination
T580 Phosphorylation
Y582 Phosphorylation
K586 Sumoylation
K586 Ubiquitination
T595 Phosphorylation
K599 Ubiquitination
S605 Phosphorylation
K624 Ubiquitination
K636 Ubiquitination
K643 Ubiquitination
K647 Sumoylation
K647 Ubiquitination
K655 Ubiquitination
K662 Sumoylation
S670 Phosphorylation
T679 Phosphorylation
S682 Phosphorylation
S684 Phosphorylation
S686 Phosphorylation
S691 Phosphorylation
S714 Phosphorylation
S716 Phosphorylation Q9NRM7 (LATS2) , O95835 (LATS1) , Q15208 (STK38)
S718 Phosphorylation
S724 Phosphorylation
T735 Phosphorylation
Y736 Phosphorylation
T738 Phosphorylation
K741 Ubiquitination
S744 Phosphorylation
K757 Ubiquitination
S812 Phosphorylation
S814 Phosphorylation Q9NRM7 (LATS2) , O95835 (LATS1) , Q15208 (STK38)
T861 Phosphorylation
S867 Phosphorylation
T873 Phosphorylation
K877 Sumoylation
K877 Ubiquitination
Y881 Phosphorylation
T883 Phosphorylation
R884 Methylation
S889 Phosphorylation
T897 Phosphorylation
K899 Ubiquitination
S902 Phosphorylation
S904 Phosphorylation
S905 Phosphorylation
S906 Phosphorylation
S911 Phosphorylation Q96GD4 (AURKB)
S912 Phosphorylation
T913 Phosphorylation
S924 Phosphorylation
T927 Phosphorylation
S943 Phosphorylation
Y949 Phosphorylation
K956 Ubiquitination

研究背景

功能:

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.

翻译修饰:

Ubiquitinated. Deubiquitinated by USP8/UBPY.

细胞定位:

Nucleus. Cytoplasm>Cytoskeleton>Spindle. Midbody>Midbody ring.
Note: Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY. Interacts with ARF6, forming heterodimers and heterotetramers.

蛋白家族:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). Diagnostic biomarker KIF23 is associated with immune infiltration and immunotherapy response in gastric cancer. Frontiers in Oncology, 2023 (PubMed: 37483517) [IF=4.7]

Application: IHC    Species: Human    Sample:

Figure 2 IHC results about KIF23 protein expression. (A, C, E–H) KIF23 expression in adjacent gastric tissues. (B, D, J–M) KIF23 expression in GC tissues. Magnification: E, G, J, H (×200); F, H, K, M (×400). (I) Rate of KIF23 expression with high and low in GC and adjacent gastric tissues.
2). Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma. American Journal of Translational Research, 2020 (PubMed: 32509165)

Application: WB    Species: human    Sample: liver cancer cell

Figure 7. | KIF2C/4A/10/11/14/18B/20A/23 promote cell proliferation in HCC. A. Eight KIFs were evaluated in liver cancer cell lines compared with the normal liver epithelial cell line HL-7702, as analyzed by qRT-PCR. B. Eight distinct KIFs were effectively knocked down by siRNAs.
3). Apoptotic Extracellular Vesicles (ApoEVs) Safeguard Liver Homeostasis and Regeneration via Assembling an ApoEV-Golgi Organelle. bioRxiv, 2021

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