REV1 Antibody - #DF2581

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产品: REV1 抗体
货号: DF2581
描述: Rabbit polyclonal antibody to REV1
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 138 kDa; 138kD(Calculated).
蛋白号: Q9UBZ9
RRID: AB_2839787

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
REV1 Antibody detects endogenous levels of total REV1.
RRID:
AB_2839787
引用格式: Affinity Biosciences Cat# DF2581, RRID:AB_2839787.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AIBP 80; AIBP80; Alpha integrin binding protein 80; Alpha integrin-binding protein 80; DNA repair protein REV 1; DNA repair protein REV1; FLJ21523; MGC163283; MGC26225; REV 1 (yeast homolog) like; REV 1; REV 1 homolog; REV 1 like; REV 1 protein; REV 1L; REV1 (yeast homolog) like; REV1; REV1 homolog (S. cerevisiae); REV1 homolog; REV1 like (yeast); REV1 like; Rev1 like terminal deoxycytidyl transferase; REV1 protein; Rev1-like terminal deoxycytidyl transferase; REV1_HUMAN; REV1L;

抗原和靶标

免疫原:
Uniprot:

Q9UBZ9(REV1_HUMAN) >>Visit HPA database.

基因/基因ID:
REV1(51455)
表达:
Q9UBZ9 REV1_HUMAN:

Ubiquitous.

描述:
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
序列:
MRRGGWRKRAENDGWETWGGYMAAKVQKLEEQFRSDAAMQKDGTSSTIFSGVAIYVNGYTDPSAEELRKLMMLHGGQYHVYYSRSKTTHIIATNLPNAKIKELKGEKVIRPEWIVESIKAGRLLSYIPYQLYTKQSSVQKGLSFNPVCRPEDPLPGPSNIAKQLNNRVNHIVKKIETENEVKVNGMNSWNEEDENNDFSFVDLEQTSPGRKQNGIPHPRGSTAIFNGHTPSSNGALKTQDCLVPMVNSVASRLSPAFSQEEDKAEKSSTDFRDCTLQQLQQSTRNTDALRNPHRTNSFSLSPLHSNTKINGAHHSTVQGPSSTKSTSSVSTFSKAAPSVPSKPSDCNFISNFYSHSRLHHISMWKCELTEFVNTLQRQSNGIFPGREKLKKMKTGRSALVVTDTGDMSVLNSPRHQSCIMHVDMDCFFVSVGIRNRPDLKGKPVAVTSNRGTGRAPLRPGANPQLEWQYYQNKILKGKAADIPDSSLWENPDSAQANGIDSVLSRAEIASCSYEARQLGIKNGMFFGHAKQLCPNLQAVPYDFHAYKEVAQTLYETLASYTHNIEAVSCDEALVDITEILAETKLTPDEFANAVRMEIKDQTKCAASVGIGSNILLARMATRKAKPDGQYHLKPEEVDDFIRGQLVTNLPGVGHSMESKLASLGIKTCGDLQYMTMAKLQKEFGPKTGQMLYRFCRGLDDRPVRTEKERKSVSAEINYGIRFTQPKEAEAFLLSLSEEIQRRLEATGMKGKRLTLKIMVRKPGAPVETAKFGGHGICDNIARTVTLDQATDNAKIIGKAMLNMFHTMKLNISDMRGVGIHVNQLVPTNLNPSTCPSRPSVQSSHFPSGSYSVRDVFQVQKAKKSTEEEHKEVFRAAVDLEISSASRTCTFLPPFPAHLPTSPDTNKAESSGKWNGLHTPVSVQSRLNLSIEVPSPSQLDQSVLEALPPDLREQVEQVCAVQQAESHGDKKKEPVNGCNTGILPQPVGTVLLQIPEPQESNSDAGINLIALPAFSQVDPEVFAALPAELQRELKAAYDQRQRQGENSTHQQSASASVPKNPLLHLKAAVKEKKRNKKKKTIGSPKRIQSPLNNKLLNSPAKTLPGACGSPQKLIDGFLKHEGPPAEKPLEELSASTSGVPGLSSLQSDPAGCVRPPAPNLAGAVEFNDVKTLLREWITTISDPMEEDILQVVKYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFILDNVQVVLQQTYGSTLKVT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
90
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UBZ9 作为底物

Site PTM Type Enzyme
K28 Ubiquitination
K99 Sumoylation
K107 Ubiquitination
K119 Ubiquitination
K134 Ubiquitination
K140 Ubiquitination
S254 Phosphorylation
S297 Phosphorylation
S301 Phosphorylation
K442 Ubiquitination
K473 Ubiquitination
Y541 Phosphorylation
Y546 Phosphorylation
S607 Phosphorylation
K625 Ubiquitination
T675 Phosphorylation
T687 Phosphorylation
K770 Ubiquitination
S812 Phosphorylation
S901 Phosphorylation
K1033 Ubiquitination
S1082 Phosphorylation
S1088 Phosphorylation
S1097 Phosphorylation
T1101 Phosphorylation
S1108 Phosphorylation

研究背景

功能:

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK. May bind ITGA3. Interacts with FAAP20/C1orf86.

蛋白家族:

The C-terminal domain is necessary for protein interactions.

Belongs to the DNA polymerase type-Y family.

研究领域

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

文献引用

1). Elevated expression of REV1 is a predictor of unfavorable prognosis in patients with prostate cancer. , 2021

Application: WB    Species: Human    Sample: Prostate cancer tissues

Figure 1. Immunostaining of REV1 protein in prostate cancer, normal prostate, and hyperplasia tissues. (A) Immunostaining of TMA from 80 samples. (B) IRSs of REV1 protein in Prostate cancer, normal prostate, and hyperplasia tissues. Expression levels of REV1 protein in Prostate cancer tissues were significantly higher than that in non-cancerous prostate tissues (IRS: Prostate cancer = 4.08±1.30 vs. Normal = 2.50±0.71, P < 0.001; Prostate cancer = 4.08±1.30 vs. Hyperplasia = 3.15±1.14, P = 0.007). **mean P < 0.01; (C, D) Representative immunohistochemical photographs of REV1 protein in Prostate cancer tissues. (E, F) Representative immunohistochemical photographs of REV1 protein in normal and hyperplasia prostate tissues. The red arrows in (C, D) show positive staining cells.

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