| 产品: | SMARCAD1 抗体 |
| 货号: | DF2598 |
| 描述: | Rabbit polyclonal antibody to SMARCAD1 |
| 应用: | WB IHC |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 117 kDa; 117kD(Calculated). |
| 蛋白号: | Q9H4L7 |
| RRID: | AB_2839804 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2598, RRID:AB_2839804.
展开/折叠
ATP dependent helicase 1; ATP-dependent helicase 1; ETL1; hHEL1; Smarcad1; SMRCD_HUMAN; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1;
抗原和靶标
Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level) (PubMed:29409814).
- Q9H4L7 SMRCD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLFNLDRFRFEKRNKIEEAPEATPQPSQPGPSSPISLSAEEENAEGEVSRANTPDSDITEKTEDSSVPETPDNERKASISYFKNQRGIQYIDLSSDSEDVVSPNCSNTVQEKTFNKDTVIIVSEPSEDEESQGLPTMARRNDDISELEDLSELEDLKDAKLQTLKELFPQRSDNDLLKLIESTSTMDGAIAAALLMFGDAGGGPRKRKLSSSSEPYEEDEFNDDQSIKKTRLDHGEESNESAESSSNWEKQESIVLKLQKEFPNFDKQELREVLKEHEWMYTEALESLKVFAEDQDMQYVSQSEVPNGKEVSSRSQNYPKNATKTKLKQKFSMKAQNGFNKKRKKNVFNPKRVVEDSEYDSGSDVGSSLDEDYSSGEEVMEDGYKGKILHFLQDASIGELTLIPQCSQKKAQKITELRPFNSWEALFTKMSKTNGLSEDLIWHCKTLIQERDVVIRLMNKCEDISNKLTKQVTMLTGNGGGWNIEQPSILNQSLSLKPYQKVGLNWLALVHKHGLNGILADEMGLGKTIQAIAFLAYLYQEGNNGPHLIVVPASTIDNWLREVNLWCPTLKVLCYYGSQEERKQIRFNIHSRYEDYNVIVTTYNCAISSSDDRSLFRRLKLNYAIFDEGHMLKNMGSIRYQHLMTINANNRLLLTGTPVQNNLLELMSLLNFVMPHMFSSSTSEIRRMFSSKTKSADEQSIYEKERIAHAKQIIKPFILRRVKEEVLKQLPPKKDRIELCAMSEKQEQLYLGLFNRLKKSINNLEKNTEMCNVMMQLRKMANHPLLHRQYYTAEKLKEMSQLMLKEPTHCEANPDLIFEDMEVMTDFELHVLCKQYRHINNFQLDMDLILDSGKFRVLGCILSELKQKGDRVVLFSQFTMMLDILEVLLKHHQHRYLRLDGKTQISERIHLIDEFNTDMDIFVFLLSTKAGGLGINLTSANVVILHDIDCNPYNDKQAEDRCHRVGQTKEVLVIKLISQGTIEESMLKINQQKLKLEQDMTTVDEGDEGSMPADIATLLKTSMGL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H4L7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K77 | Sumoylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K84 | Sumoylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| T137 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K161 | Sumoylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| K258 | Sumoylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K268 | Methylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| Y319 | Phosphorylation | Uniprot | |
| K335 | Sumoylation | Uniprot | |
| K346 | Sumoylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K352 | Acetylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| K461 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K695 | Sumoylation | Uniprot | |
| K695 | Ubiquitination | Uniprot | |
| K705 | Ubiquitination | Uniprot | |
| K712 | Ubiquitination | Uniprot | |
| K716 | Sumoylation | Uniprot | |
| K724 | Sumoylation | Uniprot | |
| K724 | Ubiquitination | Uniprot | |
| K729 | Acetylation | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| K767 | Ubiquitination | Uniprot | |
| K780 | Ubiquitination | Uniprot | |
| K796 | Ubiquitination | Uniprot | |
| K798 | Ubiquitination | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| S853 | Phosphorylation | Uniprot | |
| T880 | Phosphorylation | Uniprot | |
| Y897 | Phosphorylation | Uniprot | |
| K903 | Ubiquitination | Uniprot | |
| K970 | Ubiquitination | Uniprot | |
| K989 | Ubiquitination | Uniprot | |
| K994 | Acetylation | Uniprot |
研究背景
DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing.
Nucleus. Chromosome.
Note: Colocalizes with PCNA at replication forks during S phase. Recruited to double-strand breaks (DSBs) sites of DNA damage.
Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level).
Binds to DNA preferentially in the vicinity of transcriptional start sites. Interacts with MSH2 and TRIM28. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with PCNA.
Belongs to the SNF2/RAD54 helicase family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
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