SOX4 Antibody - #DF2610

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产品: SOX4 抗体
货号: DF2610
描述: Rabbit polyclonal antibody to SOX4
应用: WB
反应: Human, Mouse
预测: Pig, Bovine, Rabbit, Dog
分子量: 47 kDa; 47kD(Calculated).
蛋白号: Q06945
RRID: AB_2839816

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Rabbit(86%), Dog(100%)
克隆:
Polyclonal
特异性:
SOX4 Antibody detects endogenous levels of total SOX4.
RRID:
AB_2839816
引用格式: Affinity Biosciences Cat# DF2610, RRID:AB_2839816.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Ecotropic viral integration site 16; EVI 16; EVI16; Sox4; SOX4_HUMAN; SRY (sex determining region Y) box 4; SRY box containing gene 4; SRY related HMG box gene 4; Transcription factor SOX-4;

抗原和靶标

免疫原:
Uniprot:

Q06945(SOX4_HUMAN) >>Visit HPA database.

基因/基因ID:
SOX4(6659)
表达:
Q06945 SOX4_HUMAN:

Testis, brain, and heart.

描述:
Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
序列:
MVQQTNNAENTEALLAGESSDSGAGLELGIASSPTPGSTASTGGKADDPSWCKTPSGHIKRPMNAFMVWSQIERRKIMEQSPDMHNAEISKRLGKRWKLLKDSDKIPFIREAERLRLKHMADYPDYKYRPRKKVKSGNANSSSSAAASSKPGEKGDKVGGSGGGGHGGGGGGGSSNAGGGGGGASGGGANSKPAQKKSCGSKVAGGAGGGVSKPHAKLILAGGGGGGKAAAAAAASFAAEQAGAAALLPLGAAADHHSLYKARTPSASASASSAASASAALAAPGKHLAEKKVKRVYLFGGLGTSSSPVGGVGAGADPSDPLGLYEEEGAGCSPDAPSLSGRSSAASSPAAGRSPADHRGYASLRAASPAPSSAPSHASSSASSHSSSSSSSGSSSSDDEFEDDLLDLNPSSNFESMSLGSFSSSSALDRDLDFNFEPGSGSHFEFPDYCTPEVSEMISGDWLESSISNLVFTY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
86
Chicken
78
Xenopus
67
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q06945 作为底物

Site PTM Type Enzyme
S50 Phosphorylation
K53 Ubiquitination
T54 Phosphorylation
R75 Methylation
S81 Phosphorylation
K91 Ubiquitination
K95 Acetylation
K101 Ubiquitination
K105 Ubiquitination
K118 Ubiquitination
K127 Ubiquitination
S136 Phosphorylation
S143 Phosphorylation
S148 Phosphorylation
K154 Acetylation
S161 Phosphorylation
S174 Phosphorylation
S175 Phosphorylation
S185 Phosphorylation
S191 Phosphorylation
K192 Acetylation
K196 Acetylation
K197 Acetylation
K202 Ubiquitination
K213 Acetylation
K213 Ubiquitination
K217 Acetylation
K217 Ubiquitination
K261 Ubiquitination
S307 Phosphorylation
S347 Phosphorylation
S348 Phosphorylation
S354 Phosphorylation

研究背景

功能:

Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Testis, brain, and heart.

亚基结构:

Interacts with UBE2I.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice. CELL DEATH AND DIFFERENTIATION, 2024 [IF=13.7]
2). Schizandrin A can inhibit non‑small cell lung cancer cell proliferation by inducing cell cycle arrest, apoptosis and autophagy. International Journal of Molecular Medicine, 2021 (PubMed: 34643254) [IF=5.4]

Application: WB    Species: Human    Sample: A549 and H1975 cells

Figure 2 SchA induces cell cycle arrest in A549 and H1975 cells. The effect of SchA on the cell cycle progression of (A) A549 and (B) H1975 cells. The effect of SchA on the protein expression levels of cell cycle regulation proteins in (C) A549 and (D) H1975 cells. *P
3). Expression of SOX4 Significantly Predicts the Risk of Lymph Node Metastasis for Patients With Early-Stage Esophageal Squamous Cell Carcinoma. Laboratory investigation; a journal of technical methods and pathology, 2024 (PubMed: 38431117) [IF=5.0]
4). Circ_0089823 reinforces malignant behaviors of non-small cell lung cancer by acting as a sponge for microRNAs targeting SOX4. NEOPLASIA, 2021 (PubMed: 34311177) [IF=4.8]

Application: WB    Species: Human    Sample: NSCLC cells

Fig. 6. SOX4 is targeted by miR-507, miR-557, miR-579-3p and miR-1287-5p. (A-D) The binding between SOX4 and these 4 microRNAs was verified by luciferase reporter assay. (E-H) After transfection with mimics or inhibitor of these 4 microRNAs, the level of SOX4 was detected by qPCR. (I-L) The level of SOX4 in NSCLC cells was determined by western blot after transfection. The results were presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.
5). METTL3-mediated ALDH m6A methylation regulates the malignant behavior of BMI1+ HNSCC stem cells. Oral Diseases, 2023 (PubMed: 37249063) [IF=3.8]

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