PIK3C2B Antibody - #DF2624

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产品: PIK3C2B 抗体
货号: DF2624
描述: Rabbit polyclonal antibody to PIK3C2B
应用: WB IHC
反应: Human, Mouse
预测: Pig, Horse, Sheep, Rabbit, Dog
分子量: 185 kDa; 185kD(Calculated).
蛋白号: O00750
RRID: AB_2839830

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(82%), Horse(82%), Sheep(80%), Rabbit(91%), Dog(82%)
克隆:
Polyclonal
特异性:
PIK3C2B Antibody detects endogenous levels of total PIK3C2B.
RRID:
AB_2839830
引用格式: Affinity Biosciences Cat# DF2624, RRID:AB_2839830.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C2 PI3K; phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide; phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta; phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta; Phosphoinositide 3 Kinase C2 beta; Phosphoinositide 3-Kinase class 2, beta; phosphoinositide-3-kinase, class 2, beta polypeptide; PI3K C2beta; PtdIns 3 kinase C2 beta; PTDINS-3-kinase C2 beta; ptdIns-3-kinase C2 subunit beta;

抗原和靶标

免疫原:
Uniprot:

O00750(P3C2B_HUMAN) >>Visit HPA database.

基因/基因ID:
PIK3C2B(5287)
表达:
O00750 P3C2B_HUMAN:

Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney.

序列:
MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHDKEENRAKQNADPSLISWDEPGVDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSLSPQEGPPNHSTSQGPQPGSDPWPKGSLSGDYLYIFDGSDGGVSSSPGPGDIEGSCKKLSPPPLPPRASIWDTPPLPPRKGSPSSSKISQPSDINTFSLVEQLPGKLLEHRILEEEEVLGGGGQGRLLGSVDYDGINDAITRLNLKSTYDAEMLRDATRGWKEGRGPLDFSKDTSGKPVARSKTMPPQVPPRTYASRYGNRKNATPGKNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRSGSDIQDYFLTGYVWSAVTPSPEHLGDEVNLKVTVLCDRLQEALTFTCNCSSTVDLLIYQTLCYTHDDLRNVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDIDIRLQLMEQKVVRSDLARTVNDDQSPSTLNYLVHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLAVRENREKVVEALTAAILDLVELYCNTFNADFQTAVPGSRKHDLVQEACHFARSLAFTVYATHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIVWDQQICFPVQVNRLPRETLLCATLYALPIPPPGSSSEANKQRRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENPSARWSAPNFHQPDSVILQIDFPTSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDIMQKESLYWLTDADKKRLWEKRYYCHSEVSSLPLVLASAPSWEWACLPDIYVLLKQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAVSDLRVTHYFFWLLKDGLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNALAKLAQQVREAAPSARQGILRTGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLSFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGRGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYVLGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFASRTHTLKSSGRISDVFLCRHEKIFHPNKGYIYVVKVMRENTHEATYIQRTFEEFQELHNKLRLLFPSSHLPSFPSRFVIGRSRGEAVAERRREELNGYIWHLIHAPPEVAECDLVYTFFHPLPRDEKAMGTSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQLLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENVLLGEVNIRLRELDLAQEKTGWFALGSRSHGTL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
91
Pig
82
Horse
82
Dog
82
Sheep
80
Bovine
73
Chicken
64
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00750 作为底物

Site PTM Type Enzyme
T4 Phosphorylation
K12 Ubiquitination
S13 Phosphorylation
K22 Ubiquitination
Y34 Phosphorylation
K44 Ubiquitination
K50 Ubiquitination
Y68 Phosphorylation
S69 Phosphorylation
K70 Ubiquitination
K79 Ubiquitination
S115 Phosphorylation
Y127 Phosphorylation
S155 Phosphorylation
T168 Phosphorylation
S177 Phosphorylation
S179 Phosphorylation
K182 Ubiquitination
K201 Ubiquitination
S225 Phosphorylation
Y228 Phosphorylation
K241 Ubiquitination
K267 Ubiquitination
Y293 Phosphorylation
S308 Phosphorylation
K452 Ubiquitination
K465 Ubiquitination
S469 Phosphorylation
K496 Ubiquitination
K571 Ubiquitination
K617 Ubiquitination
T738 Phosphorylation
K752 Ubiquitination
K801 Ubiquitination
K818 Ubiquitination
K825 Ubiquitination
K836 Ubiquitination
K1027 Ubiquitination
K1047 Ubiquitination
K1065 Ubiquitination
K1140 Ubiquitination
T1148 Phosphorylation
S1150 Phosphorylation
K1152 Ubiquitination
K1162 Ubiquitination
K1228 Ubiquitination
K1377 Ubiquitination
K1383 Ubiquitination
Y1401 Phosphorylation
K1415 Ubiquitination
K1482 Ubiquitination
T1486 Phosphorylation
K1491 Ubiquitination
K1503 Ubiquitination
K1515 Ubiquitination
Y1541 Phosphorylation
K1552 Ubiquitination
K1563 Ubiquitination

研究背景

功能:

Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades.

细胞定位:

Microsome. Cell membrane. Cytoplasm>Cytosol. Nucleus. Endoplasmic reticulum.
Note: Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney.

亚基结构:

Part of a complex with ERBB2 and EGFR. Part of a complex with phosphorylated EGFR and GRB2. Interacts with phosphorylated EGFR and PDGFR, maybe indirectly. Interacts with GRB2 and SHC1.

蛋白家族:

Belongs to the PI3/PI4-kinase family.

研究领域

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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