RASA1 Antibody - #DF2645

FIGURE 6 | Effects of Huangqin decoction (HQD) on colonic expression levels of Ras-phosphoinositide-3-kinase (PI3K)-Akt-hypoxia inducible factor 1 alpha (HIF-1a) pathway proteins assessed by Western blot in dextran sulfate sodium (DSS)-induced colitis mice (A).

Fig. 2. miR-31-5p restrain RASA1 expression and upregulates MAP3K1 level by directly targeting the 3′ UTR of RASA1 mRNA. (A) The predicted binding sites of miR-31-5p and MAP3K1, the red parts represent the wild-type, the blue represents the mutant type. (B) Detection of dual-luciferase activity after co-transfection of NC or miR-31-5p with wild or mutant type MAP3K1. (C) The predicted binding sites of miR-31-5p and RASA1, red represents the wild-type, the blue represents the mutant-type. (D) Detection of dual-luciferase activity after co-transfection of NC or miR-31-5p with wild or mutant type RASA1. MAP3K1 (E) and RASA1 (F) mRNA expression in HFSCs after transfection with NC, goat miR-31-5p mimic, Inhibitor-NC and goat miR-31-5p Inhibitor as evidenced by qPCR. (G) MAP3K1 and RASA1 protein expression levels were examined after transfection with NC, goat miR-31-5p mimic, Inhibitor-NC and goat miR-31-5p Inhibitor in HFSCs. Note: MI: goat miR- 31-5p mimic; INC: Inhibitor-NC; IN: goat miR-31-5p Inhibitor.