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FIGURE 2 Bilirubin increased 5-HT3A receptor expression and neuron activities in the spinal cord.

产品:	HTR3E 抗体
货号:	DF2708
描述:	Rabbit polyclonal antibody to HTR3E
应用:	WB IHC
反应:	Human
分子量:	51kDa; 51kD(Calculated).
蛋白号:	A5X5Y0
RRID:	AB_2839914

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human

克隆:

Polyclonal

特异性:

HTR3E Antibody detects endogenous levels of total HTR3E.

RRID:

AB_2839914
引用格式: Affinity Biosciences Cat# DF2708, RRID:AB_2839914.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

5-HT-3; 5-HT3 receptor subunit E splice variant HTR3Ea; 5-HT3c1; 5-HT3E; 5-HT3R; 5-hydroxytryptamine (serotonin) receptor 3, family member E; 5-hydroxytryptamine receptor 3 subunit E; 5-hydroxytryptamine receptor 3E; 5HT3c1; 5HT3E; 5HT3R; HTR3; MGC120035; MGC120036; MGC120037; SEROTONIN 5-HT-3E RECEPTOR; serotonin receptor 3 subunit E; Serotonin receptor 3E;

抗原和靶标

免疫原:

Uniprot:

A5X5Y0(5HT3E_HUMAN) >>Visit HPA database.

基因/基因ID:

HTR3E(285242)

表达:

A5X5Y0 5HT3E_HUMAN:

Expressed in adult colon and intestine.

序列:

A5X5Y0 5HT3E_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEGSWFHRKRFSFYLLLGFLLQGRGVTFTINCSGFGQHGADPTALNSVFNRKPFRPVTNISVPTQVNISFAMSAILDVNEQLHLLSSFLWLEMVWDNPFISWNPEECEGITKMSMAAKNLWLPDIFIIELMDVDKTPKGLTAYVSNEGRIRYKKPMKVDSICNLDIFYFPFDQQNCTLTFSSFLYTVDSMLLDMEKEVWEITDASRNILQTHGEWELLGLSKATAKLSRGGNLYDQIVFYVAIRRRPSLYVINLLVPSGFLVAIDALSFYLPVKSGNRVPFKITLLLGYNVFLLMMSDLLPTSGTPLIGVYFALCLSLMVGSLLETIFITHLLHVATTQPPPLPRWLHSLLLHCNSPGRCCPTAPQKENKGPGLTPTHLPGVKEPEVSAGQMPGPAEAELTGGSEWTRAQREHEAQKQHSVELWLQFSHAMDAMLFRLYLLFMASSIITVICLWNT

翻译修饰 - A5X5Y0 作为底物

A5X5Y0

Site	PTM Type	Enzyme	Source
S228	Phosphorylation		Uniprot

研究背景

A5X5Y0_5HT3E_HUMAN

功能:

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.

细胞定位:

Cell membrane>Multi-pass membrane protein.
Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.

组织特异性:

Expressed in adult colon and intestine.

亚基结构:

Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex.

蛋白家族:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3E sub-subfamily.

研究领域

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

文献引用

1). Bilirubin Induces Pain Desensitization in Cholestasis by Activating 5-Hydroxytryptamine 3A Receptor in Spinal Cord. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 33869168) [IF=5.5]

Application: WB Species: Rat Sample: HEK293 cells

FIGURE 2 Bilirubin increased 5-HT3A receptor expression and neuron activities in the spinal cord. (A) 5-HT3A receptor expression increased gradually in spinal cord enlargement of BDL rats, whereas expression of 5-HT3B, 5-HT3C, 5-HT3D, and 5-HT3E receptors did not change significantly. (B) The quantitative analysis of 5-HT3A receptor expression in BDL rats. (C) Intrathecal administration of bilirubin increased 5-HT3A receptor expression without changing other subtypes. (D) The quantitative analysis of 5-HT3A receptor expression after intrathecal administration of bilirubin in normal rats. (E) SDHNs cultured with bilirubin showed increased 5-HT3A receptor expression without changing other subtypes. (F) The quantitative analysis of 5-HT3A receptor expression in SDHNs. (G) Immunofluorescence showed that 5-HT3A receptor expression increased in laminas I and II in the spinal cords of BDL rats. The increased C-Fos protein (marker of neuron activity) expression indicated that neuron activities were also enhanced in the spinal cords of BDL rats. *P < 0.05.

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HTR3E Antibody - #DF2708

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