产品: GHSR 抗体
货号: DF2794
描述: Rabbit polyclonal antibody to GHSR
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Rabbit
分子量: 41 kDa; 41kD(Calculated).
蛋白号: Q92847
RRID: AB_2840000

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(86%), Rabbit(86%)
克隆:
Polyclonal
特异性:
GHSR Antibody detects endogenous levels of total GHSR.
RRID:
AB_2840000
引用格式: Affinity Biosciences Cat# DF2794, RRID:AB_2840000.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GH releasing peptide receptor; GH-releasing peptide receptor; Ghrelin receptor; GHRP; GHRPR; GHS-R; GHSR; GHSR_HUMAN; Growth hormone secretagogue receptor 1; Growth hormone secretagogue receptor type 1; growth hormone secretatgogue receptor;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q92847 GHSR_HUMAN:

Pituitary and hypothalamus.

描述:
Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine).;
序列:
MWNATPSEEPGFNLTLADLDWDASPGNDSLGDELLQLFPAPLLAGVTATCVALFVVGIAGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIFLCMPLDLVRLWQYRPWNFGDLLCKLFQFVSESCTYATVLTITALSVERYFAICFPLRAKVVVTKGRVKLVIFVIWAVAFCSAGPIFVLVGVEHENGTDPWDTNECRPTEFAVRSGLLTVMVWVSSIFFFLPVFCLTVLYSLIGRKLWRRRRGDAVVGASLRDQNHKQTVKMLAVVVFAFILCWLPFHVGRYLFSKSFEPGSLEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVAVFRLLGFEPFSQRKLSTLKDESSRAWTESSINT

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
86
Horse
86
Sheep
75
Pig
71
Bovine
71
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q92847 作为底物

Site PTM Type Enzyme

研究背景

功能:

Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine).

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Pituitary and hypothalamus.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

文献引用

1). Maternal acrylamide exposure changes intestinal epithelium, immunolocalization of leptin and ghrelin and their receptors, and gut barrier in weaned offspring. Scientific Reports, 2023 (PubMed: 37355724) [IF=4.6]

Application: IHC    Species: Rat    Sample:

Figure 3 Quantitative analysis of the intensity of ghrelin IR in (A) villi and (B) crypts of small intestine segments, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the higher the intensity of the IR). (C) Representative images of the ghrelin IR in sections of proximal jejunum; inset: antibody control. Quantitative analysis of the intensity of ghrelin receptor IR in (D) villi and (E) crypts of small intestine segments, measured by comparison of the optical density (OD) of the microscopic images converted to 8-bit, grey-scale images. (F) Representative images of the ghrelin IR in sections of duodenum. (C), (F): All scale bars represent 100 µm. (A), (B), (D), (E): Bar plots show lsmeans value and standard deviation (whiskers). A p-value range was attributed above plots when two groups exhibit significant differences: *p value 

2). Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins. Animals, 2023 (PubMed: 37174575) [IF=3.0]

Application: IHC    Species: Rat    Sample:

Figure 2 Representative images and quantitative analysis of the intensity of ghrelin, ghrelin receptor, leptin, and leptin receptor immunoreaction (IR) in crypts and villi of the duodenum of female newborn rat offspring after maternal exposure to fumonisins (FBs) at the doses of 0, 60, or 90 mg/kg b.w. Bottom isotype row shows IHC IR control incubated without a primary antibody. All of the images were taken at the same magnification; scale bars represent 50 μm. Bar charts: intensity of immunoreaction (IR) to specific antibody was measured in crypts and villi using optical density (OD) measurements in the microscopic images. Results are presented as mean and SD. Statistically significant differences between groups are indicated by

3). Ghrelin inhibits early brain injury due to subarachnoid hemorrhage via the Tim-3-mediated HMGB1/NF-κB pathway. JOURNAL OF CHEMICAL NEUROANATOMY, 2022 (PubMed: 35863561) [IF=2.8]

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.