产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0148, RRID:AB_2833330.
展开/折叠
DNA directed DNA polymerase beta; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DPOLB_HUMAN; MGC125976; Pol B; Pol beta; POLB; Polymerase (DNA directed) beta;
抗原和靶标
- P06746 DPOLB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P06746 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| Y36 | Phosphorylation | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| K72 | Acetylation | PR:000007102 (EP300) | Uniprot |
| K81 | Acetylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| R83 | Methylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K141 | Sumoylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| R152 | Methylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y250 | Phosphorylation | Uniprot | |
| R258 | Methylation | Uniprot | |
| Y322 | Phosphorylation | Uniprot |
研究背景
Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation.
Nucleus. Cytoplasm.
Note: Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Interacts with FAM168A.
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
Belongs to the DNA polymerase type-X family.
研究领域
· Genetic Information Processing > Replication and repair > Base excision repair.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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