产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0151, RRID:AB_2833333.
展开/折叠
E2F 4; E2F transcription factor 4; E2F transcription factor 4 p107/p130 binding; E2F-4; E2F4; E2F4_HUMAN; p107/p130 binding protein; Transcription factor E2F 4; Transcription factor E2F4; E2F 5; E2F transcription factor 5; E2F transcription factor 5 p130 binding; E2F-5; E2f5; E2F5_HUMAN; p130 binding; Transcription factor E2F5;
抗原和靶标
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Q16254 E2F4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL
- Q15329 E2F5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAEPASSGQQAPAGQGQGQRPPPQPPQAQAPQPPPPPQLGGAGGGSSRHEKSLGLLTTKFVSLLQEAKDGVLDLKAAADTLAVRQKRRIYDITNVLEGIDLIEKKSKNSIQWKGVGAGCNTKEVIDRLRYLKAEIEDLELKERELDQQKLWLQQSIKNVMDDSINNRFSYVTHEDICNCFNGDTLLAIQAPSGTQLEVPIPEMGQNGQKKYQINLKSHSGPIHVLLINKESSSSKPVVFPVPPPDDLTQPSSQSLTPVTPQKSSMATQNLPEQHVSERSQALQQTSATDISSAGSISGDIIDELMSSDVFPLLRLSPTPADDYNFNLDDNEGVCDLFDVQILNY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16254/Q15329 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| Y59 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T248 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K53 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot |
研究背景
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Differentially phosphorylated in vivo.
Nucleus.
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. The E2F4/TFDP1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5). Interacts with CEBPA (when phosphorylated).
Belongs to the E2F/DP family.
Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Nucleus.
Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.
Belongs to the E2F/DP family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.