(1)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0152, RRID:AB_2833334.
展开/折叠
E2F 6; E2F binding site modulating activity protein; E2F transcription factor 6; E2F transcription factor 6 isoform 1; E2F-6; E2F6; E2F6_HUMAN; EMA; MGC111545; Transcription factor E2F6;
抗原和靶标
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
- O75461 E2F6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQQRPARKLPSLLLDPTEETVRRRCRDPINVEGLLPSKIRINLEDNVQYVSMRKALKVKRPRFDVSLVYLTRKFMDLVRSAPGGILDLNKVATKLGVRKRRVYDITNVLDGIDLVEKKSKNHIRWIGSDLSNFGAVPQQKKLQEELSDLSAMEDALDELIKDCAQQLFELTDDKENERLAYVTYQDIHSIQAFHEQIVIAVKAPAETRLDVPAPREDSITVHIRSTNGPIDVYLCEVEQGQTSNKRSEGVGTSSSESTHPEGPEEEENPQQSEELLEVSN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75461 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| S12 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| T21 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| K74 | Acetylation | Uniprot | |
| K91 | Acetylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K100 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| T221 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot |
研究背景
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Nucleus.
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10.
Belongs to the E2F/DP family.
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