Phospho-AKT1/2/3 (Ser473) Antibody - #AF0016

Figure 5: Effects of RLX on the expression of pAkt and apoptosis proteins.

$Figure 5: BVD-523 synergizes with VS-5584 in AML cells. (A) BxPC-3, MIAPaCa-2, and CFPAC-1 cells were treated with vehicle control or variable concentrations of BVD-523 and VS-5584, alone or in combination, for 48 h. Viable cells were measured by MTT assays. Standard isobologram analyses of the antitumor interactions are shown. The IC50 values of each drug are plotted on the axes; the solid line represents the additive effect, while the points represent the concentrations of each drug resulting in 50% inhibition of proliferation. Points falling below the line indicate synergism whereas those above the line indicate antagonism. (B) HPAC cells were treated with vehicle control, VS, BVD-523 (BVD), or VS plus BVD for 48 h. Cells were fxed with 80% ice-cold ethanol and stained with PI for cell cycle analysis. The percentage of cells with sub-G1 DNA content are graphed as means of triplicates ± SEM from one representative experiment. ***indicates p < 0.001; combined treatment compared to individual treatments and vehicle control. Combination index (CI) values were calculated using CompuSyn software. (C) CI vs. Fa plot (combination index vs. fraction affected) for the cell death data is presented. (D) HPAC cells were treated with vehicle control or the indicated drugs for 48 h. Whole cell lysates were subjected to Western blotting and probed with the indicated antibody. The fold changes for the densitometry measurements, normalized to β-actin and then compared to no drug treatment control, are indicated.$

Figure 5: BVD-523 synergizes with VS-5584 in AML cells.

Treatment with angelicin alters the protein expression levels of PI3K, p-Akt and total Akt in HepG2 and Huh-7 cells in vitro.

Fig 5.

(b) Western bolt shown that inhibited MEK, both decrease MNK2 expression and eIF4E phosphorylation.

FIGURE 6 |Effects of rFIP‐glu‐mediated PI3K/Akt and MAPK activation in macrophages.

Figure S1.

Figure 6—MANF promoted corneal epithelial wound healing and nerve regeneration through Akt activation.

$Fig.2 The effect of NaAsO2 on mitophagy, ox-mtDNA and NLRP3 inflammation in rats liver. Male SD rats were treated with 2.5, 5 mg/kg of NaAsO2 for 3 months. Liver coefficient (A). The level of serum ALT and AST were determined by commercial reagent kits (B-C). H&E staining of liver sections after NaAsO2 administration (D). scale bar = 500 μm. NAS in rat liver (E). Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The relative expression of MPO was shown as the percentage of GAPDH (F-G). The level of oxmtDNA was measured with an ELISA kit (H). Mitochondria fractions were analyzed by Western blot analysis. VDAC1, mitochondria marker protein, was used as an Journal Pre-proof internal control. The protein level and densitometric analyses of PINK1, Parkin, LC3B in rats liver tissues (I-L). The protein level and densitometric analyses of NLRP3, IL-1β, IL-18 expressed in rat liver tissues (M-T). Results are mean ± SEM of 5 rats. *P < 0.05 compare with the control group.$

Figure 5: |Effects of RLX on the expression of pAkt and apoptosis proteins.

Figure 4:| Efect of LQZS on phosphorylation levels of EGFR, PI3K, and AKT in lung tissues of rats with AECOPD.

Figure 4 |Effect of ursodeoxycholic acid on oleic acid-induced activation of the AKT/mTOR signaling pathway.

FIGURE 6 | Effects of Huangqin decoction (HQD) on colonic expression levels of Ras-phosphoinositide-3-kinase (PI3K)-Akt-hypoxia inducible factor 1 alpha (HIF-1a) pathway proteins assessed by Western blot in dextran sulfate sodium (DSS)-induced colitis mice (A).

Figure 8.

Representative WB images of the expression of Menin, Akt, Akt phosphorylated at Ser473, mTOR, mTOR phosphorylated at Ser2448, S6K1 and S6K1 phosphorylated at Thr389 are shown in (C).

FIGURE 5 |Effect of CT and DOX on the hepatic protein of PI3K/Akt signaling pathway in rats.

Figure 7.

FIGURE 2 Protein phosphorylation for FXYD1 and PI3K/AKT pathway components in the frontal cortex of RTT mice is altered, and IGF-1 treatment reverses the change.

Figure 6 Experimental validation of the key signaling pathways in vitro.

FIGURE 2 CUDC‐907 treatment inhibits PI3K and HDACs, but fails to inactivate ERK in prostate cancer cells.

FIGURE 5 PI3K/AKT signalling pathway was regulated by AKR1C2.

FIGURE 3|MiR-19b-3p overexpression suppressed autophagy via binging to PTEN.

Figure 6| mRNA and protein expression levels of genes related to Akt/mTOR in HepG2 cells transfected with miR-183-5p inhibitor and mimics.

Figure 6 miR-100-3p controls hMSC adipogenesis via the PI3K/AKT signaling pathway.

FIGURE 3 MiR‐19b‐3p overexpression suppressed autophagy via binging to PTEN.

FIGURE 5 FMN inhibits myostatin‐mediated dephosphorylation on the PI3K/Akt/FoxO3a signalling pathway in the muscle of CKD rats and C2C12 myotubes.

Figure 4 miR-136-5p overexpression increases the cisplatin sensitivity of laryngeal squamous cell carcinoma and head and neck squamous cell carcinoma cells.

FIGURE 7 Effects of HJF on the PI3K/AKT/mTOR signalling pathway.

Figure 4 High iodine induced the proliferation of BCPAP and 8305C cells via activating AKT-mediated Wee1/CDK1 axis.

FIGURE 3 SILENCING OF CRKL INHIBITED PI3K/AKT/MRP1 PATHWAY IN K562/ADR CELLS.

FIGURE 7 Cav-1 inhibition by CP activated the Akt/GSK3β-mediated proteasome degradation of β-catenin via ubiquitination activation.

Figure 4 HSYA inactivates PI3K/Akt signaling and enhances NO production.

Figure 6 mRNA and protein expression levels of genes related to Akt/mTOR in HepG2 cells transfected with miR-183-5p inhibitor and mimics.

Figure 3 ZJJ activated the hippocampal insulin signaling pathway in diabetic rats.

Figure 4 ZJJ activated the neuronal insulin signaling pathway in diabetic rats.

FIGURE 6 The effect of BUB1 inhibition on tumour growth of OS in vivo and the related mechanism.

Figure 8 Effect of LEO on protein expression of PI3K, p-PI3K, Akt, p-Akt, eNOS, and p-eNOS in HUVEC.

Figure 4 Notoginsenoside R1 inhibits the KRAS/PI3K/Akt pathway via YBX3.

Figure 6 Effect of BG on PI3K/Akt signaling with respect to restoring the blood-spinal cord barrier and to anti-apoptosis in SH-SY5Y cells.

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot.

Figure 7 Effect of downregulating miR-128 on the PIK3R1/Akt/mTOR pathway.

Figure 3.

Figure 3 shRac1 and shP67 transfection reduce cell death/apoptosis.

Figure 7:| Effect of downregulating miR-128 on the PIK3R1/Akt/mTOR pathway.

Figure 5 Effect of djulis hull crude extract on the expression of proteins involved in glucose transportation in (A) epididymal white adipose tissue (eWAT) and (B) the liver of high-fat diet-induced hyperglycaemia.

Figure 4 Knockdown of DEAD-box helicase 51 induced inactivation of the phosphoinositide 3-kinase/AKT signaling pathway.

Figure 7 FA inhibited the TGF-β1-induced activation of NOX4/ROS and PI3K/Akt pathway.

FIGURE 4 METTL1‐mediated m7G tRNA modification regulates the activity of the PI3K/AKT/mTOR signaling pathway.

Figure 2 Effects of high levels of glucose and fat on expression of apoptosis-related and PTEN/Akt/FOXO3a proteins in H9c2 cells.

Figure 7 The expression of PTPRH was induced by cytokine IL-13 and the effect of PTPRH on EGFR, ERK1/2, and AKT in HBECs.

Figure 5 MiR-216b modulates PC EMT through the PI3K/AKT pathway.

FIGURE 13 Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot.

Figure 6 Effect of BG on PI3K/Akt signaling with respect to restoring the blood-spinal cord barrier and to anti-apoptosis in SH-SY5Y cells.

Figure 8.

Figure 6 Periplocymarin (PPM) modulates gene expression involved in PI3K/AKT signaling pathway.

Figure 7 The effect of periplocymarin (PPM) on colorectal cancer cells in nude mice.

FIGURE 4 Effects of surfactin on key protein in insulin signaling pathway in IR‐HepG2 cells.

Figure 8 RAC prevented the activation of the PI3K/AKT/mTOR and MAPK pathway.

Figure 5 Effect of LBAG on the expression of autophagy and PI3K/AKT pathway-related proteins in rats.

Figure 5 Expression levels of PI3K/Akt/GSK-3β pathway-related proteins in intestinal tissue of NEC mice.

Figure 5 Effect of inonotsuoxide B on PDGF-BB-induced AKT, ERK1/2 and PI3K phosphorylation in hepatic stellate cells.

Figure 3 Involvement of the PI3K/Akt pathway in SPRR3-mediated regulation of ccRCC cells.

Figure 4 Anlotinib inhibits the migration of HSC-3 cells by suppressing the activation of the Akt pathway.

Figure 6 TIPE3 promotes NSCLC progression by an AKT/ERK1/2-GSK3β-β-catenin/Snail axis.

Figure 4 Baicalin inhibited the PDK1/AKT signaling pathway in NSCLC.

4 小鼠卵巢颗粒细胞中p-PI3K、PI3K、p-Akt及Akt蛋白表达情况 Protein expression of p-PI3K, PI3K, p-Akt, and Akt in ovarian granulosa cells in different groups (Mean±SD, n=3).

Figure 5 The miR-17-5p agomir was administrated via the tail vein 24 h before I/R modeling (n=4 per group).

FIGURE 8 S1PR3 inhibition changes the levels of Akt, p38, and ERK1/2 phosphorylation.

FIGURE 4 CA reverses the EMT process of A2780 and SKOV3 via the PI3K/AKT signaling pathway.

Figure 5 Effects of ICA on impaired insulin signaling in the cerebral cortex of 3×Tg-AD mice.

Figure 6 EO regulated Akt/p38 MAPK pathway-related proteins in human TNBC cells.

Figure 3 Lycorine changes the activation level of the Akt signaling pathway via RBM10.

Figure 5 KRT17 regulates LSCC progression by affecting AKT/mTOR and Wnt/β-catenin axes (a).

Figure 5 Analysis of the transcriptome and protein expressions in TSPCs treated with different concentrations of UA.

Figure 6 The effect of tumor-derived exosomes on miR-3960-overexpressed pancreatic cancer (PC) on the xenograft tumor model.

Figure 2 Constitutive activation of FGFR2 (p.

Figure 6 LAMC2 activated the integrin β1/FAK/Src/AKT signaling pathway in TU177 and AMC-HN-8 cells.

Figure 10 APL extract upregulated PI3K/Akt signaling of Iso-induced mice.

FIGURE 4 Effects of ELA on TGF-β1-induced activation of the SMAD/ERK/AKT Pathway.

FIGURE 7 LASP1 mediated DSY down-regulation of integrin expression in TGF-β2-treated cells.

Figure 4 Effects of p38 MAPK and Akt on ANP secretion and dynamics induced by CCK-8s in isolated beating rat atria.

FIGURE 3 Molecular profiling for iAs-induced changes in Caco-2 cells.

FIGURE 7 Validation of the mechanisms of GLXB treatment against AS in vivo.

Figure 5 Showing how MT uses the PI3K/AKT/GSK-3β/P70S6k signaling pathway to treat iron-overloaded MC3T3-E1 cells.

Figure 3 Effects of ginsenoside Rg1 on the PI3K/AKT signaling pathway and expressions of cyclins and CDKs in hAD-MSCs.

Figure 9.

FIGURE 4 The expression of PI3K/Akt‐mTOR signal pathway in four groups mice: (a) the schematic diagram of expression levels in p‐mTOR, mTOR, p‐PIK3, PI3K, p‐Akt(473), p‐Aky(308), and Akt, measured by Western blot; (b–e) the semiquantitative analysis of the expression of p‐mTOR (b), p‐PI3K (c), p‐Akt(473) (d), and p‐Akt(308) (e).

Figure 6 Enhanced angiogenic potential of ASCs via the SDF-1α/CXCR4 pathway and potentially involved mechanisms (A) The transcriptional levels of SDF-1α, CXCR4, VEGF and VEGR2 were significantly elevated in bladder augmented with ASCs-seeded BAMG-SF compared with bladder augmented with acellular BAMG-SF.

FIGURE 7 Luteolin alleviates Adriamycin (ADR)-induced renal injury and modulates AKT phosphorylation.

Figure 4 Expression levels of podocyte actin cytoskeleton components.

Figure 6 EZH2 may have functions in the AKT/ERK-mTOR pathways: (A) The relative abundance of PI3K, AKT1, AKT2, AKT3, and mTOR in PCs when there was EZH2 gene silencing.

FIGURE 3 Altered mRNA expression of downstream target genes enriched in KEGG pathways, and expression of HIPPO, MAPK, and PI3K/Akt in each study group.

FIG.

Figure 4 The effects of MT on the expression of GAD67, synaptic-associated proteins, and the PI3K/Akt signaling pathway in Ctnnd2 KO mice.