产品: FLI1 抗体
货号: AF0161
描述: Rabbit polyclonal antibody to FLI1
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 50kDa; 51kD(Calculated).
蛋白号: Q01543
RRID: AB_2833342

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
FLI1 Antibody detects endogenous levels of total FLI1.
RRID:
AB_2833342
引用格式: Affinity Biosciences Cat# AF0161, RRID:AB_2833342.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ERGB transcription factor; Ewing Sarcoma breakpoint region 2; EWSR 2; EWSR2; FLI 1; FLI 1 proto oncogene; Fli-1 proto-oncogene, ETS transcription factor; FLI1; FLI1 EWS fusion gene; FLI1 proto oncogene; FLI1_HUMAN; Friend leukemia integration 1 (FLI1) transcription factor; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; Sarcoma breakpoint region 2 (EWSR2); SIC 1; SIC1; Transcription factor ERGB; Viral integration region FLI1; Viral integration region FLI1, mouse, homolog of;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
FLI1 Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Belongs to the ETS family. Can form homodimers or heterodimers with ETV6/TEL1. 2 isoforms of the human protein are produced by alternative splicing.
序列:
MDGTIKEALSVVSDDQSLFDSAYGAAAHLPKADMTASGSPDYGQPHKINPLPPQQEWINQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q01543 作为底物

Site PTM Type Enzyme
S10 Phosphorylation
S13 Phosphorylation
S21 Phosphorylation
T35 Phosphorylation
S37 Phosphorylation
S39 Phosphorylation
Y42 Phosphorylation
K47 Ubiquitination
K67 Sumoylation
Y70 Phosphorylation
S76 Phosphorylation
S79 Phosphorylation
S86 Phosphorylation
K87 Ubiquitination
S89 Phosphorylation
K172 Ubiquitination
K217 Ubiquitination
S221 Phosphorylation
Y222 Phosphorylation
S224 Phosphorylation
S236 Phosphorylation
K240 Acetylation
K240 Ubiquitination
S241 Phosphorylation
K252 Acetylation
Y263 Phosphorylation
T312 Phosphorylation Q05655 (PRKCD)
K325 Ubiquitination
Y332 Phosphorylation
K334 Ubiquitination
K350 Ubiquitination
K380 Acetylation
K380 Ubiquitination
K397 Acetylation
R438 Methylation
S450 Phosphorylation
Y451 Phosphorylation
Y452 Phosphorylation

研究背景

功能:

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Can form homodimers or heterodimers with ETV6/TEL1.

蛋白家族:

Belongs to the ETS family.

研究领域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.