(3)
(8)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0212, RRID:AB_2833344.
展开/折叠
Matrix metallopeptidase 14 (membrane inserted); Matrix metalloproteinase 14; Matrix metalloproteinase-14; Membrane type 1 matrix metalloproteinase; Membrane type 1 metalloprotease; Membrane type matrix metalloproteinase 1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP 14; MMP X1; MMP-14; MMP-X1; Mmp14; MMP14_HUMAN; MMPX1; MT MMP 1; MT-MMP 1; MT1 MMP; MT1-MMP; MT1MMP; MTMMP 1; MTMMP1;
抗原和靶标
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
- P50281 MMP14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSPAPRPPRCLLLPLLTLGTALASLGSAQSSSFSPEAWLQQYGYLPPGDLRTHTQRSPQSLSAAIAAMQKFYGLQVTGKADADTMKAMRRPRCGVPDKFGAEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRVWESATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTPFDGEGGFLAHAYFPGPNIGGDTHFDSAEPWTVRNEDLNGNDIFLVAVHELGHALGLEHSSDPSAIMAPFYQWMDTENFVLPDDDRRGIQQLYGGESGFPTKMPPQPRTTSRPSVPDKPKNPTYGPNICDGNFDTVAMLRGEMFVFKERWFWRVRNNQVMDGYPMPIGQFWRGLPASINTAYERKDGKFVFFKGDKHWVFDEASLEPGYPKHIKELGRGLPTDKIDAALFWMPNGKTYFFRGNKYYRFNEELRAVDSEYPKNIKVWEGIPESPRGSFMGSDEVFTYFYKGNKYWKFNNQKLKVEPGYPKSALRDWMGCPSGGRPDEGTEEETEVIIIEVDEEGGGAVSAAAVVLPVLLLLLVLAVGLAVFFFRRHGTPRRLLYCQRSLLDKV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P50281 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T77 | O-Glycosylation | Uniprot | |
| T77 | Phosphorylation | Uniprot | |
| Y138 | Phosphorylation | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| T291 | O-Glycosylation | Uniprot | |
| T299 | O-Glycosylation | Uniprot | |
| T300 | O-Glycosylation | Uniprot | |
| S301 | O-Glycosylation | Uniprot | |
| S304 | O-Glycosylation | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| T567 | Phosphorylation | P05129 (PRKCG) | Uniprot |
| Y573 | Phosphorylation | P12931 (SRC) , P53667 (LIMK1) | Uniprot |
| S577 | Phosphorylation | Uniprot | |
| K581 | Ubiquitination | Uniprot |
研究背景
Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity). May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.
The precursor is cleaved by a furin endopeptidase.
Tyrosine phosphorylated by PKDCC/VLK.
Membrane>Single-pass type I membrane protein. Melanosome. Cytoplasm.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Interacts (via C-terminal cytoplasmic tail) with BST2. Interacts with DLL1; inhibits DLL1-induced Notch signaling.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Belongs to the peptidase M10A family.
研究领域
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
文献引用
Application: WB Species: Horse Sample: liver
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