MMP3 Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	MMP3 抗体
货号:	AF0217
描述:	Rabbit polyclonal antibody to MMP3
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Horse, Sheep, Rabbit, Dog
分子量:	53kDa; 54kD(Calculated).
蛋白号:	P08254
RRID:	AB_2833347

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Horse(100%), Sheep(87%), Rabbit(92%), Dog(93%)

克隆:

Polyclonal

特异性:

MMP3 Antibody detects endogenous levels of total MMP3.

RRID:

AB_2833347
引用格式: Affinity Biosciences Cat# AF0217, RRID:AB_2833347.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP 3; MMP-3; MMP3; MMP3_HUMAN; Proteoglycanase; SL-1; SL1; STMY; STMY1; STR1; Stromelysin 1; Stromelysin-1; Transin 1; Transin-1;

抗原和靶标

免疫原:

Uniprot:

P08254(MMP3_HUMAN) >>Visit HPA database.

基因/基因ID:

MMP3(4314)

描述:

MMP3 Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Belongs to the peptidase M10A family. Note: This description may include information from UniProtKB.

序列:

P08254 MMP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKSLPILLLLCVAVCSAYPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Horse

100

Dog

Rabbit

Sheep

Xenopus

Pig

Bovine

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P08254 作为底物

P08254

Site	PTM Type	Source
Y18	Phosphorylation	Uniprot
Y116	Phosphorylation	Uniprot
Y121	Phosphorylation	Uniprot
K127	Ubiquitination	Uniprot

研究背景

P08254_MMP3_HUMAN

功能:

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

蛋白家族:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

研究领域

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

文献引用

1). Sustained therapeutic effects of self-assembled hyaluronic acid nanoparticles loaded with α-Ketoglutarate in various osteoarthritis stages. Biomaterials, 2024 (PubMed: 39326362) [IF=14.0]

2). Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation. PHYTOMEDICINE, 2021 (PubMed: 34371251) [IF=7.9]

Application: WB Species: Rat Sample: chondrocytes

Fig. 2. Effects of avicularin on ECM degradation. (A-D) The mRNA levels of ACAN, Collagen 2, MMP3 and MMP13 in rat and human chondrocytes were detected by qPCR. The dates were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the IL-1β-induced group, n = 3. (E-F) The expression of MMP3, MMP13, ADAMTS5, SOX9, ACAN and Collagen II was detected by western blots.

Application: IHC Species: Rat Sample: chondrocytes

Fig. 7. Avicularin attenuates ECM degradation in ACLT rats. (A) ACAN, (B) Collagen II, (C) MMP3, (D) MMP13 and (E) TRAF6 immunohistochemistry of knee joint medial compartment cartilage. (F-J) Quantitative analysis of ACAN, Collagen II, MMP3, MMP13 and TRAF6. The values were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the ACLT group, n = 6. (K) The working model of how avicularin protects against OA development.

3). Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stress. PHYTOMEDICINE, 2022 (PubMed: 35905566) [IF=7.9]

4). Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota. Phytomedicine, 2022 (PubMed: 36162242) [IF=7.9]

5). Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34311530) [IF=7.5]

6). Vitamin K2 ameliorates osteoarthritis by suppressing ferroptosis and extracellular matrix degradation through activation GPX4's dual functions. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38759289) [IF=7.5]

Application: WB Species: Rat Sample:

Fig. 3. VK2 rescues rat primary chondrocytes from TBHP-Induced cell death and ECM degradation. (A) The working concentration of VK in vitro was screened by CCK8; (B) Flow cytometry detection of apoptosis; (C) Flow cytometry statistics for detection of apoptosis; (D) Representative pictures of chondrocytes of toluidine blue staining (Scale bar=200μm); (E-J) WB results for ACAN, COL2A1, SOX9, MMP3, MMP13 and Tubulin.

7). Biochanin A protects against iron overload associated knee osteoarthritis via regulating iron levels and NRF2/System xc-/GPX4 axis. Biomedicine & Pharmacotherapy, 2023 (PubMed: 36379122) [IF=7.5]

8). A non-retinol retinoic acid receptor-γ (RAR-γ/NR1B3) selective agonist, tectorigenin, can effectively inhibit the ultraviolet A-induced skin damage. British journal of pharmacology, 2022 (PubMed: 35731978) [IF=7.3]

9). White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy. British journal of pharmacology, 2024 (PubMed: 38644540) [IF=7.3]

10). Quercetin-3-O-β-D-glucuronide attenuates osteoarthritis by inhibiting cartilage extracellular matrix degradation and inflammation. Journal of orthopaedic translation, 2024 (PubMed: 38601200) [IF=6.6]

Application: WB Species: Rat Sample: chondrocytes

Figure 2. Q3GA on the expression of genes and proteins in the extracellular matrix (ECM) and inflammation in chondrocytes. (A) QRT–PCR was used to detect the mRNA expression of Col2a1, Acan, Adamts5, Mmp13, and Mmp3, n = 3. (B) Western blots were used to detect the protein expression of Col2a1, ACAN, ADAMTS5, MMP13, and MMP3, n = 3. (C) QRT–PCR was used to detect the mRNA expression of Inos and Cox2, n = 3. (D) Western blots were used to detect the protein expression of iNOS and COX2, n = 3. *P < 0.05, **P < 0.01, and ***P < 0.001, compared with the control group; #P < 0.05, ##P < 0.01, and ###P < 0.001, compared with the IL-1β induced group.

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MMP3 Antibody - #AF0217

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