产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0586, RRID:AB_2834148.
展开/折叠
DnaJ (Hsp40) homolog subfmaily B member 1; DNAJ 1; DNAJ B1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB 1; Dnajb1; DNAJB1 protein; DNJB1_HUMAN; HDJ 1; HDJ-1; HDJ1; Heat shock 40 kDa protein 1; Heat shock 40kD protein 1; Heat shock protein 40; Hsp 40; HSP40; HSPF 1; HSPF1; Human DnaJ protein 1; Radial spoke 16 homolog B; RSPH16B; Sis1;
抗原和靶标
- P25685 DNJB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P25685 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Ubiquitination | Uniprot | |
| Y5 | Phosphorylation | Uniprot | |
| R13 | Methylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K44 | Acetylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| R66 | Methylation | Uniprot | |
| T88 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Q8IW41 (MAPKAPK5) | Uniprot |
| S151 | Phosphorylation | Q8IW41 (MAPKAPK5) | Uniprot |
| K159 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Q8IW41 (MAPKAPK5) | Uniprot |
| Y176 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| K181 | Acetylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K195 | Acetylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| Y256 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| T307 | Phosphorylation | Uniprot |
研究背景
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).
Cytoplasm. Nucleus. Nucleus>Nucleolus.
Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
Interacts with DNAJC3. Interacts with HSF1 (via transactivation domain); this interaction results in the inhibition of heat shock- and HSF1-induced transcriptional activity during the attenuation and recovery phase period of the heat shock response.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Infectious diseases: Viral > Influenza A.
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