Cytokeratin 14/16 Antibody - #AF0736

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产品: Cytokeratin 14/16 抗体
货号: AF0736
描述: Rabbit polyclonal antibody to Cytokeratin 14/16
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Rabbit, Dog
分子量: 52kDa; 52kD,51kD(Calculated).
蛋白号: P02533 | P08779
RRID: AB_2834150

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(80%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Cytokeratin 14/16 Antibody detects endogenous levels of total Cytokeratin 14/16.
RRID:
AB_2834150
引用格式: Affinity Biosciences Cat# AF0736, RRID:AB_2834150.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CK 14; CK-14; ck14; Cytokeratin 14; Cytokeratin-14; Cytokeratin14; Dowling Meara; EBS3; EBS4; Epidermolysis bullosa simplex; K14; K1C14_HUMAN; Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); Keratin 14; Keratin; Keratin type I cytoskeletal 14; Keratin, type I cytoskeletal 14; Keratin-14; Keratin14; Koebner; Krt 14; Krt14; NFJ; OTTHUMP00000164624; type I cytoskeletal 14; CK 16; CK-16; CK16; Cytokeratin-16; Cytokeratin16; FNEPPK; Focal non epidermolytic palmoplantar keratoderma; K 16; K16; K1C16_HUMAN; K1CP; Keratin 1 type I; Keratin 16; Keratin; Keratin type I cytoskeletal 16; Keratin-16; Keratin16; KRT 16; Krt16; KRT16A; NEPPK; PC1; type I cytoskeletal 16;

抗原和靶标

免疫原:
Uniprot:

P02533(K1C14_HUMAN) >>Visit HPA database.

P08779(K1C16_HUMAN) >>Visit HPA database.

基因/基因ID:
KRT14(3861) | KRT16(3868)
表达:
P02533 K1C14_HUMAN:

Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.

P08779 K1C16_HUMAN:

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

描述:
K14 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5. Note: This description may include information from UniProtKB.
序列:
MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSSRFSSGGACGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGAGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN

MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGAGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Dog
100
Pig
80
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02533/P08779 作为底物

Site PTM Type Enzyme
T9 Phosphorylation
S10 Phosphorylation
S12 Phosphorylation
S13 Phosphorylation
K15 Methylation
S17 Phosphorylation
S28 Phosphorylation
S32 Phosphorylation
S33 Phosphorylation
S39 Phosphorylation
S44 Phosphorylation
T45 Phosphorylation
Y46 Phosphorylation
S51 Phosphorylation
S54 Phosphorylation
S130 Phosphorylation
Y131 Phosphorylation
K134 Acetylation
K134 Ubiquitination
Y153 Phosphorylation
K162 Acetylation
S165 Phosphorylation
Y167 Phosphorylation
K169 Acetylation
T204 Phosphorylation
Y206 Phosphorylation
T215 Phosphorylation
T235 Phosphorylation
Y250 Phosphorylation
Y293 Phosphorylation
K311 Ubiquitination
K317 Ubiquitination
S321 Phosphorylation
S329 Phosphorylation
S360 Phosphorylation
K365 Ubiquitination
K407 Ubiquitination
T416 Phosphorylation
Y417 Phosphorylation
Y438 Phosphorylation
S449 Phosphorylation
S450 Phosphorylation
S451 Phosphorylation
S452 Phosphorylation
K460 Methylation
S463 Phosphorylation
S464 Phosphorylation
S468 Phosphorylation
S472 Phosphorylation
S473 Phosphorylation
Site PTM Type Enzyme
T9 Phosphorylation
S10 Phosphorylation
S12 Phosphorylation
S13 Phosphorylation
K15 Methylation
S17 Phosphorylation
S28 Phosphorylation
S32 Phosphorylation
S33 Phosphorylation
S39 Phosphorylation
S44 Phosphorylation
T45 Phosphorylation
Y46 Phosphorylation
S53 Phosphorylation
S128 Phosphorylation
Y129 Phosphorylation
K132 Acetylation
K132 Ubiquitination
Y151 Phosphorylation
S163 Phosphorylation
Y165 Phosphorylation
Y204 Phosphorylation
S213 Phosphorylation
K243 Methylation
Y248 Phosphorylation
K250 Sumoylation
K250 Ubiquitination
K251 Ubiquitination
Y291 Phosphorylation
K293 Ubiquitination
T319 Phosphorylation
S321 Phosphorylation
S326 Phosphorylation
K328 Ubiquitination
S332 Phosphorylation
S358 Phosphorylation
K363 Ubiquitination
Y398 Phosphorylation
K399 Ubiquitination
K405 Ubiquitination
T414 Phosphorylation
Y415 Phosphorylation
S429 Phosphorylation
S432 Phosphorylation
S433 Phosphorylation
S435 Phosphorylation
S437 Phosphorylation
S438 Phosphorylation

研究背景

功能:

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

翻译修饰:

A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.

Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.

细胞定位:

Cytoplasm. Nucleus.
Note: Expressed in both as a filamentous pattern.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.

亚基结构:

Heterotetramer of two type I and two type II keratins. disulfide-linked keratin-14 associates with keratin-5. Interacts with TRADD and with keratin filaments. Associates with other type I keratins. Interacts with EPPK1 (By similarity). Interacts with KLHL24.

蛋白家族:

Belongs to the intermediate filament family.

功能:

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

组织特异性:

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

亚基结构:

Heterodimer of a type I and a type II keratin. KRT16 associates with KRT6 isomers (KRT6A or KRT6B) (By similarity). Interacts with TCHP. Interacts with TRADD (By similarity).

蛋白家族:

Belongs to the intermediate filament family.

研究领域

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

文献引用

1). Targeting Anaerobic Respiration in Pseudomonas aeruginosa with Chlorate Improves Healing of Chronic Wounds. Advances in wound care, 2024 (PubMed: 37432895) [IF=4.9]

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