Ki67 Antibody - #AF0198

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产品: Ki67 抗体
货号: AF0198
描述: Rabbit polyclonal antibody to Ki67
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Rabbit, Dog
分子量: 358kDa; 359kD(Calculated).
蛋白号: P46013
RRID: AB_2834152

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Rabbit(100%), Dog(82%)
克隆:
Polyclonal
特异性:
Ki67 Antibody detects endogenous levels of total Ki67.
RRID:
AB_2834152
引用格式: Affinity Biosciences Cat# AF0198, RRID:AB_2834152.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen KI67; Antigen Ki67; KI67_HUMAN; KIA; Marker of proliferation Ki-67; MIB 1; MIB; MKI67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen; Protein phosphatase 1 regulatory subunit 105; RP11-380J17.2;

抗原和靶标

免疫原:
Uniprot:

P46013(KI67_HUMAN) >>Visit HPA database.

基因/基因ID:
MKI67(4288)
描述:
KI-67 a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.
序列:
MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDIRIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFRYENESLQNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNPQVHIKNVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSRYGELKSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATEKESADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKAVGASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLTPRKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLTQVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSFGGHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSEIHVEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNLPSKRVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQRSMNKRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQKMDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLPTSESFGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLETKTSDTETEPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEMKEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTARGQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLAVGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSLEDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEEEFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEKAQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQTPKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTPLEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKRHPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSKRSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGSKRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSKRRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAASLTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTPTSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLDQPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDPADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPVEKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSPQPDPVKTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFKESAKQMLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIACKSPPPESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFRETAKQKLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIACRSPQPDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDMKAFMGTPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPPLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVVIMKRSLRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQITEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNESSQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVTRSVKRCAENPKKAEDNVCVKKIRTRSHRDSEDI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Bovine
100
Dog
82
Chicken
55
Xenopus
45
Pig
0
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P46013 作为底物

Site PTM Type Enzyme
K11 Ubiquitination
K46 Ubiquitination
K83 Ubiquitination
R93 Methylation
K107 Ubiquitination
S108 Phosphorylation
T109 Phosphorylation
K114 Ubiquitination
S125 Phosphorylation
S127 Phosphorylation
S128 Phosphorylation
S130 Phosphorylation
S131 Phosphorylation
K136 Ubiquitination
K145 Ubiquitination
K150 Ubiquitination
K160 Ubiquitination
K163 Sumoylation
K163 Ubiquitination
K172 Ubiquitination
S184 Phosphorylation
S185 Phosphorylation
K204 Sumoylation
K204 Ubiquitination
K210 Ubiquitination
K219 Methylation
K219 Ubiquitination
K231 Ubiquitination
K232 Ubiquitination
S235 Phosphorylation
K239 Ubiquitination
Y241 Phosphorylation
S243 Phosphorylation
K245 Sumoylation
K245 Ubiquitination
K251 Ubiquitination
K254 Ubiquitination
K263 Ubiquitination
S264 Phosphorylation
T268 Phosphorylation
K274 Ubiquitination
S296 Phosphorylation
S299 Phosphorylation
S308 Phosphorylation
K317 Ubiquitination
S325 Phosphorylation
T328 Phosphorylation
S330 Phosphorylation
K331 Ubiquitination
S336 Phosphorylation
Y340 Phosphorylation
K344 Ubiquitination
K346 Ubiquitination
T347 Phosphorylation
S352 Phosphorylation
S357 Phosphorylation P24941 (CDK2)
K360 Ubiquitination
K364 Ubiquitination
Y367 Phosphorylation
T368 Phosphorylation
S374 Phosphorylation Q96GD4 (AURKB)
K379 Acetylation
K379 Ubiquitination
K388 Acetylation
T389 Phosphorylation
T391 Phosphorylation
S396 Phosphorylation
T401 Phosphorylation
S411 Phosphorylation
T413 Phosphorylation
K414 Ubiquitination
K421 Ubiquitination
S425 Phosphorylation
K458 Ubiquitination
S460 Phosphorylation
K466 Acetylation
K502 Methylation
K502 Ubiquitination
S507 Phosphorylation
T525 Phosphorylation
K528 Ubiquitination
K537 Ubiquitination
S538 Phosphorylation Q96GD4 (AURKB)
T543 Phosphorylation
K548 Ubiquitination
K552 Sumoylation
K552 Ubiquitination
K560 Ubiquitination
S563 Phosphorylation
S565 Phosphorylation
S575 Phosphorylation
S579 Phosphorylation
S584 Phosphorylation
K587 Sumoylation
K587 Ubiquitination
T588 Phosphorylation
S592 Phosphorylation
S598 Phosphorylation
T601 Phosphorylation
S605 Phosphorylation
K608 Ubiquitination
T611 Phosphorylation
K615 Ubiquitination
K620 Ubiquitination
S621 Phosphorylation
K627 Ubiquitination
S634 Phosphorylation
K645 Ubiquitination
S648 Phosphorylation
S651 Phosphorylation
K659 Ubiquitination
S660 Phosphorylation
K666 Ubiquitination
K670 Ubiquitination
K675 Ubiquitination
T694 Phosphorylation
K696 Ubiquitination
K697 Ubiquitination
S704 Phosphorylation
S707 Phosphorylation
S713 Phosphorylation
T716 Phosphorylation
K721 Ubiquitination
K726 Ubiquitination
K745 Sumoylation
K745 Ubiquitination
K749 Sumoylation
K749 Ubiquitination
S753 Phosphorylation
K760 Ubiquitination
T761 Phosphorylation P06493 (CDK1)
K764 Ubiquitination
K818 Ubiquitination
S825 Phosphorylation
S827 Phosphorylation
T844 Phosphorylation
Y849 Phosphorylation
K850 Ubiquitination
S853 Phosphorylation
K857 Ubiquitination
T858 Phosphorylation
S859 Phosphorylation
K867 Ubiquitination
S878 Phosphorylation
K886 Ubiquitination
K892 Sumoylation
K892 Ubiquitination
K906 Ubiquitination
K910 Ubiquitination
K923 Ubiquitination
K933 Ubiquitination
K957 Ubiquitination
K968 Ubiquitination
S969 Phosphorylation
K977 Ubiquitination
T988 Phosphorylation
K993 Ubiquitination
K1004 Ubiquitination
T1017 Phosphorylation
K1022 Ubiquitination
K1026 Ubiquitination
S1028 Phosphorylation
K1031 Ubiquitination
K1035 Sumoylation
K1035 Ubiquitination
K1043 Ubiquitination
K1063 Acetylation
K1063 Ubiquitination
T1067 Phosphorylation
K1069 Sumoylation
S1071 Phosphorylation
K1073 Ubiquitination
T1091 Phosphorylation
K1093 Sumoylation
K1093 Ubiquitination
S1098 Phosphorylation
K1106 Ubiquitination
T1111 Phosphorylation
S1115 Phosphorylation
S1118 Phosphorylation
S1131 Phosphorylation
S1136 Phosphorylation
T1139 Phosphorylation
T1141 Phosphorylation
S1142 Phosphorylation
T1143 Phosphorylation
K1144 Ubiquitination
K1153 Sumoylation
K1153 Ubiquitination
K1165 Acetylation
K1165 Ubiquitination
T1167 Phosphorylation
S1169 Phosphorylation
K1172 Ubiquitination
T1176 Phosphorylation
K1178 Acetylation
K1185 Acetylation
K1188 Sumoylation
K1188 Ubiquitination
T1193 Phosphorylation
K1197 Ubiquitination
T1203 Phosphorylation
S1207 Phosphorylation
K1208 Acetylation
K1208 Ubiquitination
T1213 Phosphorylation
K1217 Ubiquitination
K1228 Ubiquitination
T1233 Phosphorylation
T1237 Phosphorylation
K1245 Ubiquitination
K1248 Ubiquitination
C1251 S-Nitrosylation
S1253 Phosphorylation
S1256 Phosphorylation
T1261 Phosphorylation
T1263 Phosphorylation
S1264 Phosphorylation
T1265 Phosphorylation
K1266 Ubiquitination
S1272 Phosphorylation
K1275 Ubiquitination
S1291 Phosphorylation
T1298 Phosphorylation
S1302 Phosphorylation
T1315 Phosphorylation
T1323 Phosphorylation
T1327 Phosphorylation
S1329 Phosphorylation
K1330 Acetylation
K1330 Ubiquitination
T1335 Phosphorylation
K1337 Ubiquitination
T1347 Phosphorylation
K1350 Ubiquitination
T1355 Phosphorylation
T1359 Phosphorylation
K1367 Ubiquitination
S1375 Phosphorylation
S1376 Phosphorylation
S1380 Phosphorylation
T1383 Phosphorylation
T1385 Phosphorylation
S1386 Phosphorylation
T1387 Phosphorylation
T1393 Phosphorylation
K1402 Ubiquitination
S1405 Phosphorylation
K1408 Acetylation
K1409 Ubiquitination
T1411 Phosphorylation
S1414 Phosphorylation
K1422 Ubiquitination
K1441 Ubiquitination
S1447 Phosphorylation
T1449 Phosphorylation
S1451 Phosphorylation
K1452 Acetylation
K1452 Ubiquitination
K1460 Ubiquitination
K1471 Ubiquitination
T1476 Phosphorylation
T1480 Phosphorylation
K1482 Acetylation
K1482 Ubiquitination
T1485 Phosphorylation
S1496 Phosphorylation
T1503 Phosphorylation
T1505 Phosphorylation
S1506 Phosphorylation
S1507 Phosphorylation
K1508 Ubiquitination
S1511 Phosphorylation
K1512 Methylation
K1512 Ubiquitination
S1514 Phosphorylation
K1517 Sumoylation
K1517 Ubiquitination
T1531 Phosphorylation
S1533 Phosphorylation
T1540 Phosphorylation
K1542 Ubiquitination
S1546 Phosphorylation
K1549 Ubiquitination
Y1552 Phosphorylation
T1557 Phosphorylation
T1565 Phosphorylation
T1569 Phosphorylation
S1571 Phosphorylation
K1572 Acetylation
K1572 Ubiquitination
T1577 Phosphorylation
K1581 Acetylation
K1581 Ubiquitination
K1592 Acetylation
K1592 Ubiquitination
T1597 Phosphorylation
T1601 Phosphorylation
S1604 Phosphorylation
K1612 Acetylation
S1618 Phosphorylation
S1628 Phosphorylation
S1629 Phosphorylation
S1636 Phosphorylation
K1639 Acetylation
K1639 Ubiquitination
K1643 Sumoylation
K1643 Ubiquitination
K1651 Ubiquitination
S1656 Phosphorylation
T1660 Phosphorylation
S1679 Phosphorylation
S1686 Phosphorylation
S1689 Phosphorylation
T1691 Phosphorylation
S1693 Phosphorylation
K1694 Ubiquitination
T1699 Phosphorylation
K1703 Sumoylation
K1703 Ubiquitination
S1704 Phosphorylation
T1719 Phosphorylation
S1721 Phosphorylation
K1724 Ubiquitination
S1726 Phosphorylation
K1734 Ubiquitination
S1736 Phosphorylation
Y1737 Phosphorylation
S1740 Phosphorylation
T1747 Phosphorylation
T1749 Phosphorylation
S1750 Phosphorylation
S1751 Phosphorylation
K1752 Ubiquitination
S1758 Phosphorylation
K1761 Ubiquitination
T1764 Phosphorylation O14757 (CHEK1)
T1775 Phosphorylation
S1777 Phosphorylation
T1784 Phosphorylation
K1793 Ubiquitination
T1797 Phosphorylation
T1801 Phosphorylation
K1805 Ubiquitination
S1815 Phosphorylation
K1825 Ubiquitination
T1841 Phosphorylation
T1844 Phosphorylation
T1848 Phosphorylation
T1849 Phosphorylation
K1852 Ubiquitination
T1853 Phosphorylation
K1860 Methylation
K1860 Ubiquitination
S1861 Phosphorylation
S1864 Phosphorylation
T1869 Phosphorylation
T1871 Phosphorylation
K1874 Ubiquitination
S1880 Phosphorylation
K1883 Sumoylation
K1883 Ubiquitination
K1895 Ubiquitination
T1897 Phosphorylation
S1899 Phosphorylation
K1902 Acetylation
K1902 Ubiquitination
T1906 Phosphorylation
K1915 Ubiquitination
T1919 Phosphorylation
T1923 Phosphorylation
K1927 Ubiquitination
S1937 Phosphorylation
K1938 Acetylation
K1938 Ubiquitination
T1943 Phosphorylation P06493 (CDK1)
K1949 Ubiquitination
K1958 Ubiquitination
T1963 Phosphorylation
T1967 Phosphorylation
S1970 Phosphorylation
S1980 Phosphorylation
K1982 Ubiquitination
S1983 Phosphorylation
T1991 Phosphorylation
S2002 Phosphorylation
K2005 Acetylation
K2005 Ubiquitination
K2009 Sumoylation
K2009 Ubiquitination
T2019 Phosphorylation
T2021 Phosphorylation
T2025 Phosphorylation
T2026 Phosphorylation
T2028 Phosphorylation
T2065 Phosphorylation
K2067 Sumoylation
K2067 Ubiquitination
S2072 Phosphorylation
K2080 Ubiquitination
T2085 Phosphorylation
T2089 Phosphorylation
S2105 Phosphorylation
S2110 Phosphorylation
T2113 Phosphorylation
T2115 Phosphorylation
S2116 Phosphorylation
T2123 Phosphorylation
S2135 Phosphorylation
K2138 Ubiquitination
K2167 Ubiquitination
S2173 Phosphorylation
T2175 Phosphorylation
K2178 Acetylation
K2178 Ubiquitination
K2187 Ubiquitination
K2198 Ubiquitination
T2203 Phosphorylation
T2207 Phosphorylation
T2211 Phosphorylation
T2212 Phosphorylation
T2217 Phosphorylation
S2223 Phosphorylation
T2231 Phosphorylation
T2233 Phosphorylation
K2236 Ubiquitination
S2239 Phosphorylation
K2240 Methylation
S2242 Phosphorylation
K2245 Ubiquitination
S2252 Phosphorylation
T2259 Phosphorylation
S2261 Phosphorylation
K2264 Acetylation
T2268 Phosphorylation
K2280 Acetylation
K2280 Sumoylation
T2285 Phosphorylation
K2289 Acetylation
K2289 Ubiquitination
S2299 Phosphorylation
K2300 Acetylation
K2300 Ubiquitination
T2305 Phosphorylation
K2309 Ubiquitination
K2320 Ubiquitination
T2325 Phosphorylation
T2328 Phosphorylation
K2330 Ubiquitination
T2332 Phosphorylation
T2333 Phosphorylation
T2337 Phosphorylation
K2343 Methylation
K2343 Ubiquitination
S2344 Phosphorylation
T2352 Phosphorylation
S2355 Phosphorylation
T2356 Phosphorylation
K2366 Sumoylation
K2366 Ubiquitination
T2380 Phosphorylation
S2382 Phosphorylation
T2389 Phosphorylation
S2395 Phosphorylation
K2398 Sumoylation
K2398 Ubiquitination
T2402 Phosphorylation
T2406 Phosphorylation
K2410 Ubiquitination
S2420 Phosphorylation
K2421 Acetylation
K2421 Ubiquitination
T2426 Phosphorylation
K2430 Sumoylation
K2430 Ubiquitination
K2441 Ubiquitination
T2446 Phosphorylation
T2450 Phosphorylation
S2453 Phosphorylation
T2455 Phosphorylation
T2460 Phosphorylation
S2463 Phosphorylation
S2466 Phosphorylation
S2471 Phosphorylation
K2473 Methylation
K2488 Ubiquitination
K2492 Sumoylation
K2500 Ubiquitination
T2502 Phosphorylation
T2504 Phosphorylation
S2505 Phosphorylation
T2508 Phosphorylation
T2509 Phosphorylation
T2516 Phosphorylation
K2520 Acetylation
K2523 Acetylation
S2528 Phosphorylation
K2530 Ubiquitination
S2538 Phosphorylation
S2542 Phosphorylation
K2587 Ubiquitination
S2588 Phosphorylation
T2594 Phosphorylation
T2598 Phosphorylation
T2600 Phosphorylation
K2601 Ubiquitination
K2613 Sumoylation
K2613 Ubiquitination
K2634 Ubiquitination
S2638 Phosphorylation
T2683 Phosphorylation
T2692 Phosphorylation
S2695 Phosphorylation
T2697 Phosphorylation
S2708 Phosphorylation
T2716 Phosphorylation
T2717 Phosphorylation
S2719 Phosphorylation
T2720 Phosphorylation
K2734 Sumoylation
T2749 Phosphorylation
T2750 Phosphorylation
K2767 Ubiquitination
S2769 Phosphorylation
T2773 Phosphorylation
T2781 Phosphorylation
S2783 Phosphorylation
S2793 Phosphorylation
S2814 Phosphorylation
T2816 Phosphorylation
S2827 Phosphorylation
S2828 Phosphorylation
T2834 Phosphorylation
T2836 Phosphorylation
S2837 Phosphorylation
S2838 Phosphorylation
K2852 Sumoylation
K2852 Ubiquitination
T2862 Phosphorylation
K2892 Ubiquitination
S2901 Phosphorylation
S2920 Phosphorylation
S2925 Phosphorylation
T2927 Phosphorylation
T2931 Phosphorylation
S2941 Phosphorylation
T2949 Phosphorylation
S2952 Phosphorylation
K2967 Ubiquitination
K2986 Acetylation
K2997 Acetylation
K2997 Methylation
T3007 Phosphorylation
T3009 Phosphorylation
S3041 Phosphorylation
S3042 Phosphorylation
S3067 Phosphorylation
S3082 Phosphorylation
K3087 Ubiquitination
T3127 Phosphorylation
S3128 Phosphorylation
K3180 Ubiquitination
S3197 Phosphorylation
T3213 Phosphorylation
S3216 Phosphorylation
K3217 Ubiquitination
S3218 Phosphorylation
T3223 Phosphorylation
S3249 Phosphorylation
S3253 Phosphorylation

研究背景

功能:

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

翻译修饰:

Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.

细胞定位:

Chromosome. Nucleus. Nucleus>Nucleolus.
Note: Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with KIF15. Interacts (via the FHA domain) with NIFK. Interacts with PPP1CC. Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity.

文献引用

1). Mitochondrial Disruption Nanosystem Simultaneously Depressed Programmed Death Ligand-1 and Transforming Growth Factor-β to Overcome Photodynamic Immunotherapy Resistance. ACS nano, 2024 (PubMed: 38227812) [IF=17.1]
2). AIMP1 promotes multiple myeloma malignancy through interacting with ANP32A to mediate histone H3 acetylation. Cancer Communications, 2022 (PubMed: 36042007) [IF=16.2]
3). METTL13 Mediates the Translation of Snail in Head and Neck Squamous Cell Carcinoma. International Journal of Oral Science, 2020 (PubMed: 34381012) [IF=14.9]

Application: IHC    Species: mouse    Sample: tumor

Fig. 6| METTL13 regulates HNSCC cell proliferation in vivo.d The tumor weigh was measured after sacrificing mice.e Representative images of H&E and immunohistochemistry (IHC) detection of METTL13, Snail, and Ki67 in xenograft studies. Scale bar, 200 μm

Application: IHC    Species: mouse    Sample: tumor

Fig. 6| METTL13 regulates HNSCC cell proliferation in vivo.e Representative images of H&E and immunohistochemistry (IHC) detection of METTL13, Snail, and Ki67 in xenograft studies. Scale bar, 200 μm

Application: IHC    Species: Mice    Sample: SCC15 cells

Fig. 6 METTL13 regulates HNSCC cell proliferation in vivo. a BALB/C nude mice (n = 6) were subcutaneously transplanted with SCC15 cells transfected with LV-shRNA-1, LV-shRNA-2, and LV-shCtrl. b Tumors were collected after 4 weeks. c Tumor volume was calculated every 2 days and determined by length × width2/2. d The tumor weigh was measured after sacrificing mice. e Representative images of H&E and immunohistochemistry (IHC) detection of METTL13, Snail, and Ki67 in xenograft studies. Scale bar, 200 μm
4). Mitochondrial metabolism blockade nanoadjuvant reversed immune-resistance microenvironment to sensitize albumin-bound paclitaxel-based chemo-immunotherapy. Acta pharmaceutica Sinica. B, 2024 (PubMed: 39309498) [IF=14.5]

Application: IHC    Species: Mouse    Sample: 4T1 cells

Figure 5. TPP-TAM@Alb nanoparticles reduced the expression of PD-L1 and TGF-β protein to amplify T cell infiltration in 4T1 subcutaneous tumors for enhancing the tumor cell killing activity of PTX@Alb. (A, B) The typical image of the PD-L1 and TGF-β proteins in 4T1 tumors detected by Western blot assay after different treatments. (C–E) Quantification of the amounts of CD3+, CD8+, and CD4+ T cells in 4T1 tumors by the flow cytometry measurements after receiving various treatments (n = 5). (F) The representative immunofluorescence images of Ki67 protein, TUNEL, and H&E staining after PTX@Alb, TPP-TAM@Alb, anti-PD-L1 antibody, or PTX@Alb and TPP-TAM@Alb combination therapy, scale bar = 100 μm. (G, H) Quantification of the relative Ki67 and TUNEL mean fluorescence intensity (MFI) after different treatments (n = 3). All data are presented as mean ± SD. ∗P < 0.05 and ∗∗∗P < 0.001.
5). NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma. Acta Pharmaceutica Sinica B, 2022 (PubMed: 35967285) [IF=14.5]
6). Chemical conjugation mitigates immunotoxicity of chemotherapy via reducing receptor-mediated drug leakage from lipid nanoparticles. Science advances, 2024 (PubMed: 38838152) [IF=13.6]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5. Antitumor immunity and immunotoxicity of sHDLs. (A and B) Concentrations of IFN-γ, IL-12p40, and TNF-α (A) and percentage of mature DC (B) in DLN after different treatments. (C to G) Intratumoral density of CD11b+ cells (C), M1-to-M2 ratio (D), densities of CD3+ cells (E), and CD8+IFN-γ+ cells (F) and percentage of Ki67+ cells among CD8+ cells (G) after different treatments. (H and I) Concentrations of CXCL9 (H) and CXCL10 (I) in tumors after different treatments. (J to M) Density of GMP in a single femur (J), amounts of CD11b+ cells (K), and CD3+ cells (L) in blood, and number of CD3+ cells in DLN (M) after different treatments. (N) Immunofluorescence images of DLN collected from mice receiving different treatments. DAPI (blue), Ki67 (red), CD3 (pink), and CD20 (green). Data were presented as mean ± SD (n = 5), and statistical significance was calculated using a two-sided one-way ANOVA test.
7). Identification of HDAC9 as a viable therapeutic target for the treatment of gastric cancer. EXPERIMENTAL AND MOLECULAR MEDICINE, 2019 (PubMed: 31451695) [IF=12.8]

Application: IHC    Species: mouse    Sample: SGC-7901cells

Fig. 8| HDAC9 inhibition enhanced the effect of cisplatin on GC tumor suppression.d HDAC9 expression and cell proliferation and apoptosis were assessed by HDAC9 and Ki67 immunohistochemical staining and a TUNEL assay using serial sections. The HDAC9-negative area denoted by the dotted line indicates the location of siHDAC9 injection. Scale bars = 100 μm. The error bars indicate the means ± SDs; *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group
8). BUB1 drives the occurrence and development of bladder cancer by mediating the STAT3 signaling pathway. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2021 (PubMed: 34852826) [IF=11.3]
9). Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model. Nano Research, 2021 [IF=9.9]
10). Smart dual responsive nanocarriers with reactive oxygen species amplification assisted synergistic chemotherapy against prostate cancer. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2022 (PubMed: 35544966) [IF=9.9]
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