MMP8 Antibody - #AF0219

>>买2送1 进行中>>
(1)   (7)  
产品: MMP8 抗体
货号: AF0219
描述: Rabbit polyclonal antibody to MMP8
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse
分子量: 55kDa; 53kD(Calculated).
蛋白号: P22894
RRID: AB_2833349

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(80%)
克隆:
Polyclonal
特异性:
MMP8 Antibody detects endogenous levels of total MMP8.
RRID:
AB_2833349
引用格式: Affinity Biosciences Cat# AF0219, RRID:AB_2833349.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CLG 1; CLG1; Collagenase 1; Collagenase 1 neutrophil; HNC; Matrix metallopeptidase 8 (neutrophil collagenase); Matrix metalloprotease 8; Matrix metalloproteinase-8; MMP 8; MMP-8; Mmp8; MMP8_HUMAN; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNL-CL; PMNLCL;

抗原和靶标

免疫原:
Uniprot:

P22894(MMP8_HUMAN) >>Visit HPA database.

基因/基因ID:
MMP8(4317)
表达:
P22894 MMP8_HUMAN:

Neutrophils.

描述:
MMP8 Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.
序列:
MFSLKTLPFLLLLHVQISKAFPVSSKEKNTKTVQDYLEKFYQLPSNQYQSTRKNGTNVIVEKLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDSGGFMLTPGNPKWERTNLTYRIRNYTPQLSEAEVERAIKDAFELWSVASPLIFTRISQGEADINIAFYQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTPKPCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPTGIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAIDAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAVFQQEHFFHVFSGPRYYAFDLIAQRVTRVARGNKWLNCRYG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
80
Bovine
70
Sheep
70
Rabbit
70
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P22894 作为底物

Site PTM Type Enzyme
T30 Phosphorylation
T32 Phosphorylation
Y36 Phosphorylation
Y383 Phosphorylation
S385 Phosphorylation
S422 Phosphorylation

研究背景

功能:

Can degrade fibrillar type I, II, and III collagens.

细胞定位:

Cytoplasmic granule. Secreted>Extracellular space>Extracellular matrix.
Note: Stored in intracellular granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Neutrophils.

蛋白家族:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

文献引用

1). RPRM negatively regulates ATM levels through its nuclear translocation on irradiation mediated by CDK4/6 and IPO11. iScience, 2021 (PubMed: 36185355) [IF=5.8]

Application: IF/ICC    Species: Human    Sample: H460-RPRM cells

Figure 3 RPRM promotes the nuclear export and degradation of ATM (A and B) Kinetics of the change in the ATM levels of H460-NC/RPRM cells with and without irradiation in the presence of cycloheximide (CHX, 0.1 mg/mL) examined by immunoblotting. (∗, RPRM vs NC; #, RPRM + IR vs NC + IR; &, RPRM + IR vs RPRM). (C and D) Change in the ATM levels of H460-NC (N)/RPRM (R) cells at the indicated time points after 2 Gy X-irradiation with and without MG132 (10 μM) pre-treatment. (E) Change in the subcellular localization of ATM in RPRM−/− MEFs transfected with NC and RPRM vectors after 20 Gy X-irradiation and quantification of the ratios of cytoplasmic ATM fluorescence intensity to nuclear ATM fluorescence intensity. Scale bars, 10 μm. (F) Representative immunofluorescence images of ATM in H460-RPRM cells pre-treated with leptomycin B (LMB, 10 nM) for 1 h at different times after 2 Gy X-irradiation and quantification of the cytoplasmic/nuclear ATM fluorescence intensity ratios. Scale bar, 20 μm. (G) The effect of LMB pre-treatment on the total ATM levels of H460-NC/RPRM cells at different times after 2 Gy X-irradiation. (H) Effect of LMB on the micronucleus formation of H460-NC/RPRM cells irradiated with 2 Gy X-rays.

Application: IF/ICC    Species: Human    Sample: H460-RPRM cells

Figure 3 RPRM promotes the nuclear export and degradation of ATM (A and B) Kinetics of the change in the ATM levels of H460-NC/RPRM cells with and without irradiation in the presence of cycloheximide (CHX, 0.1 mg/mL) examined by immunoblotting. (∗, RPRM vs NC; #, RPRM + IR vs NC + IR; &, RPRM + IR vs RPRM). (C and D) Change in the ATM levels of H460-NC (N)/RPRM (R) cells at the indicated time points after 2 Gy X-irradiation with and without MG132 (10 μM) pre-treatment. (E) Change in the subcellular localization of ATM in RPRM−/− MEFs transfected with NC and RPRM vectors after 20 Gy X-irradiation and quantification of the ratios of cytoplasmic ATM fluorescence intensity to nuclear ATM fluorescence intensity. Scale bars, 10 μm. (F) Representative immunofluorescence images of ATM in H460-RPRM cells pre-treated with leptomycin B (LMB, 10 nM) for 1 h at different times after 2 Gy X-irradiation and quantification of the cytoplasmic/nuclear ATM fluorescence intensity ratios. Scale bar, 20 μm. (G) The effect of LMB pre-treatment on the total ATM levels of H460-NC/RPRM cells at different times after 2 Gy X-irradiation. (H) Effect of LMB on the micronucleus formation of H460-NC/RPRM cells irradiated with 2 Gy X-rays.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.