MMP8 Antibody - #AF0219

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产品: MMP8 抗体
货号: AF0219
描述: Rabbit polyclonal antibody to MMP8
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse
分子量: 55kDa; 53kD(Calculated).
蛋白号: P22894
RRID: AB_2833349

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(80%)
克隆:
Polyclonal
特异性:
MMP8 Antibody detects endogenous levels of total MMP8.
RRID:
AB_2833349
引用格式: Affinity Biosciences Cat# AF0219, RRID:AB_2833349.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CLG 1; CLG1; Collagenase 1; Collagenase 1 neutrophil; HNC; Matrix metallopeptidase 8 (neutrophil collagenase); Matrix metalloprotease 8; Matrix metalloproteinase-8; MMP 8; MMP-8; Mmp8; MMP8_HUMAN; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNL-CL; PMNLCL;

抗原和靶标

免疫原:
Uniprot:

P22894(MMP8_HUMAN) >>Visit HPA database.

基因/基因ID:
MMP8(4317)
表达:
P22894 MMP8_HUMAN:

Neutrophils.

描述:
MMP8 Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.
序列:
MFSLKTLPFLLLLHVQISKAFPVSSKEKNTKTVQDYLEKFYQLPSNQYQSTRKNGTNVIVEKLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDSGGFMLTPGNPKWERTNLTYRIRNYTPQLSEAEVERAIKDAFELWSVASPLIFTRISQGEADINIAFYQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTPKPCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPTGIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAIDAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAVFQQEHFFHVFSGPRYYAFDLIAQRVTRVARGNKWLNCRYG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
80
Bovine
70
Sheep
70
Rabbit
70
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P22894 作为底物

Site PTM Type Enzyme
T30 Phosphorylation
T32 Phosphorylation
Y36 Phosphorylation
Y383 Phosphorylation
S385 Phosphorylation
S422 Phosphorylation

研究背景

功能:

Can degrade fibrillar type I, II, and III collagens.

细胞定位:

Cytoplasmic granule. Secreted>Extracellular space>Extracellular matrix.
Note: Stored in intracellular granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Neutrophils.

蛋白家族:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

文献引用

1). RPRM negatively regulates ATM levels through its nuclear translocation on irradiation mediated by CDK4/6 and IPO11. iScience, 2021 (PubMed: 36185355) [IF=5.8]

Application: IF/ICC    Species: Human    Sample: H460-RPRM cells

Figure 3 RPRM promotes the nuclear export and degradation of ATM (A and B) Kinetics of the change in the ATM levels of H460-NC/RPRM cells with and without irradiation in the presence of cycloheximide (CHX, 0.1 mg/mL) examined by immunoblotting. (∗, RPRM vs NC; #, RPRM + IR vs NC + IR; &, RPRM + IR vs RPRM). (C and D) Change in the ATM levels of H460-NC (N)/RPRM (R) cells at the indicated time points after 2 Gy X-irradiation with and without MG132 (10 μM) pre-treatment. (E) Change in the subcellular localization of ATM in RPRM−/− MEFs transfected with NC and RPRM vectors after 20 Gy X-irradiation and quantification of the ratios of cytoplasmic ATM fluorescence intensity to nuclear ATM fluorescence intensity. Scale bars, 10 μm. (F) Representative immunofluorescence images of ATM in H460-RPRM cells pre-treated with leptomycin B (LMB, 10 nM) for 1 h at different times after 2 Gy X-irradiation and quantification of the cytoplasmic/nuclear ATM fluorescence intensity ratios. Scale bar, 20 μm. (G) The effect of LMB pre-treatment on the total ATM levels of H460-NC/RPRM cells at different times after 2 Gy X-irradiation. (H) Effect of LMB on the micronucleus formation of H460-NC/RPRM cells irradiated with 2 Gy X-rays.

Application: IF/ICC    Species: Human    Sample: H460-RPRM cells

Figure 3 RPRM promotes the nuclear export and degradation of ATM (A and B) Kinetics of the change in the ATM levels of H460-NC/RPRM cells with and without irradiation in the presence of cycloheximide (CHX, 0.1 mg/mL) examined by immunoblotting. (∗, RPRM vs NC; #, RPRM + IR vs NC + IR; &, RPRM + IR vs RPRM). (C and D) Change in the ATM levels of H460-NC (N)/RPRM (R) cells at the indicated time points after 2 Gy X-irradiation with and without MG132 (10 μM) pre-treatment. (E) Change in the subcellular localization of ATM in RPRM−/− MEFs transfected with NC and RPRM vectors after 20 Gy X-irradiation and quantification of the ratios of cytoplasmic ATM fluorescence intensity to nuclear ATM fluorescence intensity. Scale bars, 10 μm. (F) Representative immunofluorescence images of ATM in H460-RPRM cells pre-treated with leptomycin B (LMB, 10 nM) for 1 h at different times after 2 Gy X-irradiation and quantification of the cytoplasmic/nuclear ATM fluorescence intensity ratios. Scale bar, 20 μm. (G) The effect of LMB pre-treatment on the total ATM levels of H460-NC/RPRM cells at different times after 2 Gy X-irradiation. (H) Effect of LMB on the micronucleus formation of H460-NC/RPRM cells irradiated with 2 Gy X-rays.
2). Puerarin inhibits inflammation and lipid accumulation in alcoholic liver disease through regulating MMP8. Chinese journal of natural medicines, 2023 (PubMed: 37777317) [IF=4.6]

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