产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0561, RRID:AB_2834162.
展开/折叠
AR interacting protein; DDXBP1; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; GBP; Gu binding protein; Gu-binding protein; GU/RH-II; Pias1; PIAS1_HUMAN; Protein inhibitor of activated STAT protein 1; Protein inhibitor of activated STAT, 1; RNA helicase II binding protein; RNA helicase II-binding protein; Zinc finger, MIZ-type containing 3; ZMIZ3;
抗原和靶标
- O75925 PIAS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGTWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISLD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75925 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| K8 | Sumoylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| K30 | Ubiquitination | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K40 | Sumoylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K46 | Sumoylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K56 | Sumoylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K58 | Sumoylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| K117 | Sumoylation | Uniprot | |
| K137 | Sumoylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K152 | Sumoylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K238 | Sumoylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| T291 | Phosphorylation | Uniprot | |
| R303 | Methylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K315 | Sumoylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K419 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S467 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S468 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S476 | Phosphorylation | Uniprot | |
| T480 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| T487 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| T508 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
研究背景
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).
Sumoylated.
Nucleus speckle. Nucleus>PML body.
Note: Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
Expressed in numerous tissues with highest level in testis.
Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation (By similarity). Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with CHUK/IKKA; this interaction induces PIAS1 phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5. Interacts with PML (By similarity). Interacts with MTA1. Interacts with SUMO1P1/SUMO5.
The LXXLL motif is a transcriptional coregulator signature.
The SP-RING-type domain is required for promoting EKLF sumoylation.
Belongs to the PIAS family.
研究领域
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
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