SENP6 Antibody - #AF0277

Figure 3 SENP6−reduced HUVECs attenuate AGE−induced angiogenic signaling. (A) Heat map of mRNA showing the differences in SENPs expression between the control and AGEs groups. “Red” indicates up-regulation and “Green” indicates doregulation. ** indicates that p < 0.01 and *** indicates that p < 0.001 between the indicated groups. (B) Detection of SENP6 mRNA expression by qPCR at 0, 4, 8, and 12 h of AGE−stimulated HUVECs, n = 4, * p < 0.05 vs. 0 h. (C) The temporal effect of AGE stimulation on HUVECs was investigated using Western blot, which showed SENP6 protein expression at 0, 4, 8, and 12 h. (D) Analysis of protein expression level results, n = 6, * p < 0.05 vs. 0 h. AGE−induced angiogenic responses were inhibited by SENP6 silencing (Supplemental Figure S1C). HUVECs were transfected with NC siRNA or SENP6 siRNA for 48 h. Cells were subjected to HUVECs migration and tube formation in response to AGEs. (E) Transwell migration assay for the migration ability. (F) Analysis of migration assay results, n = 3, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm. (G) HUVECs tube formation in a Matrigel assay. Representative images are shown in (G). (H,I) Analysis of total tube length and total branching points results, n = 4, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm (J) HUVECs migration by a scratch assay for indicated times. (K) Wound healing (% closure) was quantified, n = 5, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm. * and # indicate significance between the indicated groups.