NCoR1 Antibody - #AF0270

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产品: NCoR1 抗体
货号: AF0270
描述: Rabbit polyclonal antibody to NCoR1
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 270kDa; 270kD(Calculated).
蛋白号: O75376
RRID: AB_2833351

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Bovine(93%), Horse(93%), Sheep(93%), Rabbit(93%), Dog(93%), Chicken(93%)
克隆:
Polyclonal
特异性:
NCoR1 Antibody detects endogenous levels of total NCoR1.
RRID:
AB_2833351
引用格式: Affinity Biosciences Cat# AF0270, RRID:AB_2833351.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

hN CoR; hNCoR; KIAA1047; N CoR; N Cor/SMRT corepressor Rip13; N CoR1; N-CoR; N-CoR1; NCOR 1; NCoR; Ncor1; NCOR1_HUMAN; Nuclear receptor co repressor 1; Nuclear receptor corepressor 1; Retinoid X receptor interacting protein 13; RIP13; Rxrip13; thyroid hormone and retinoic acid receptor associated corepressor 1; TRAC 1; TRAC1;

抗原和靶标

免疫原:
Uniprot:

O75376(NCOR1_HUMAN) >>Visit HPA database.

基因/基因ID:
NCOR1(9611)
描述:
N-CoR1 is a protein that regulates the activity of some transcription factors, including nuclear receptors, by promoting histone deacetylation and chromatin condensation, thereby altering the accessibility of various genes to the transcriptional machinery. Is known to play a role genetic programming including that for myogenesis. Is a component of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid and the retinoic acid receptors in the absence of ligand. Interacts with the catalytic domain of HDAC9.
序列:
MSSSGYPPNQGAFSTEQSRYPPHSVQYTFPNTRHQQEFAVPDYRSSHLEVSQASQLLQQQQQQQLRRRPSLLSEFHPGSDRPQERRTSYEPFHPGPSPVDHDSLESKRPRLEQVSDSHFQRVSAAVLPLVHPLPEGLRASADAKKDPAFGGKHEAPSSPISGQPCGDDQNASPSKLSKEELIQSMDRVDREIAKVEQQILKLKKKQQQLEEEAAKPPEPEKPVSPPPVEQKHRSIVQIIYDENRKKAEEAHKIFEGLGPKVELPLYNQPSDTKVYHENIKTNQVMRKKLILFFKRRNHARKQREQKICQRYDQLMEAWEKKVDRIENNPRRKAKESKTREYYEKQFPEIRKQREQQERFQRVGQRGAGLSATIARSEHEISEIIDGLSEQENNEKQMRQLSVIPPMMFDAEQRRVKFINMNGLMEDPMKVYKDRQFMNVWTDHEKEIFKDKFIQHPKNFGLIASYLERKSVPDCVLYYYLTKKNENYKALVRRNYGKRRGRNQQIARPSQEEKVEEKEEDKAEKTEKKEEEKKDEEEKDEKEDSKENTKEKDKIDGTAEETEEREQATPRGRKTANSQGRRKGRITRSMTNEAAAASAAAAAATEEPPPPLPPPPEPISTEPVETSRWTEEEMEVAKKGLVEHGRNWAAIAKMVGTKSEAQCKNFYFNYKRRHNLDNLLQQHKQKTSRKPREERDVSQCESVASTVSAQEDEDIEASNEEENPEDSEVEAVKPSEDSPENATSRGNTEPAVELEPTTETAPSTSPSLAVPSTKPAEDESVETQVNDSISAETAEQMDVDQQEHSAEEGSVCDPPPATKADSVDVEVRVPENHASKVEGDNTKERDLDRASEKVEPRDEDLVVAQQINAQRPEPQSDNDSSATCSADEDVDGEPERQRMFPMDSKPSLLNPTGSILVSSPLKPNPLDLPQLQHRAAVIPPMVSCTPCNIPIGTPVSGYALYQRHIKAMHESALLEEQRQRQEQIDLECRSSTSPCGTSKSPNREWEVLQPAPHQVITNLPEGVRLPTTRPTRPPPPLIPSSKTTVASEKPSFIMGGSISQGTPGTYLTSHNQASYTQETPKPSVGSISLGLPRQQESAKSATLPYIKQEEFSPRSQNSQPEGLLVRAQHEGVVRGTAGAIQEGSITRGTPTSKISVESIPSLRGSITQGTPALPQTGIPTEALVKGSISRMPIEDSSPEKGREEAASKGHVIYEGKSGHILSYDNIKNAREGTRSPRTAHEISLKRSYESVEGNIKQGMSMRESPVSAPLEGLICRALPRGSPHSDLKERTVLSGSIMQGTPRATTESFEDGLKYPKQIKRESPPIRAFEGAITKGKPYDGITTIKEMGRSIHEIPRQDILTQESRKTPEVVQSTRPIIEGSISQGTPIKFDNNSGQSAIKHNVKSLITGPSKLSRGMPPLEIVPENIKVVERGKYEDVKAGETVRSRHTSVVSSGPSVLRSTLHEAPKAQLSPGIYDDTSARRTPVSYQNTMSRGSPMMNRTSDVTISSNKSTNHERKSTLTPTQRESIPAKSPVPGVDPVVSHSPFDPHHRGSTAGEVYRSHLPTHLDPAMPFHRALDPAAAAYLFQRQLSPTPGYPSQYQLYAMENTRQTILNDYITSQQMQVNLRPDVARGLSPREQPLGLPYPATRGIIDLTNMPPTILVPHPGGTSTPPMDRITYIPGTQITFPPRPYNSASMSPGHPTHLAAAASAEREREREREKERERERIAAASSDLYLRPGSEQPGRPGSHGYVRSPSPSVRTQETMLQQRPSVFQGTNGTSVITPLDPTAQLRIMPLPAGGPSISQGLPASRYNTAADALAALVDAAASAPQMDVSKTKESKHEAARLEENLRSRSAAVSEQQQLEQKTLEVEKRSVQCLYTSSAFPSGKPQPHSSVVYSEAGKDKGPPPKSRYEEELRTRGKTTITAANFIDVIITRQIASDKDARERGSQSSDSSSSLSSHRYETPSDAIEVISPASSPAPPQEKLQTYQPEVVKANQAENDPTRQYEGPLHHYRPQQESPSPQQQLPPSSQAEGMGQVPRTHRLITLADHICQIITQDFARNQVSSQTPQQPPTSTFQNSPSALVSTPVRTKTSNRYSPESQAQSVHHQRPGSRVSPENLVDKSRGSRPGKSPERSHVSSEPYEPISPPQVPVVHEKQDSLLLLSQRGAEPAEQRNDARSPGSISYLPSFFTKLENTSPMVKSKKQEIFRKLNSSGGGDSDMAAAQPGTEIFNLPAVTTSGSVSSRGHSFADPASNLGLEDIIRKALMGSFDDKVEDHGVVMSQPMGVVPGTANTSVVTSGETRREEGDPSPHSGGVCKPKLISKSNSRKSKSPIPGQGYLGTERPSSVSSVHSEGDYHRQTPGWAWEDRPSSTGSTQFPYNPLTMRMLSSTPPTPIACAPSAVNQAAPHQQNRIWEREPAPLLSAQYETLSDSDD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
93
Bovine
93
Sheep
93
Dog
93
Chicken
93
Rabbit
93
Xenopus
64
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75376 作为底物

Site PTM Type Enzyme
Y20 Phosphorylation
S70 Phosphorylation P17612 (PRKACA) , P17252 (PRKCA)
S73 Phosphorylation
T87 Phosphorylation
S88 Phosphorylation
Y89 Phosphorylation
S97 Phosphorylation
S106 Phosphorylation
K145 Ubiquitination
S157 Phosphorylation
S158 Phosphorylation
S161 Phosphorylation
S172 Phosphorylation
S174 Phosphorylation
K178 Ubiquitination
S224 Phosphorylation
K260 Sumoylation
K260 Ubiquitination
K273 Ubiquitination
Y275 Phosphorylation
K280 Ubiquitination
K294 Ubiquitination
Y341 Phosphorylation
Y342 Phosphorylation
K344 Ubiquitination
R365 Methylation
S388 Phosphorylation
S401 Phosphorylation
K445 Ubiquitination
K451 Ubiquitination
Y477 Phosphorylation
Y478 Phosphorylation
S509 Phosphorylation
K533 Acetylation
T568 Phosphorylation
R570 Methylation
T586 Phosphorylation
S588 Phosphorylation
T590 Phosphorylation
S737 Phosphorylation
S821 Phosphorylation
K842 Acetylation
K965 Ubiquitination
S989 Phosphorylation
S990 Phosphorylation
S992 Phosphorylation
S997 Phosphorylation
K998 Ubiquitination
S999 Phosphorylation
T1061 Phosphorylation
K1106 Sumoylation
S1111 Phosphorylation
S1117 Phosphorylation
S1143 Phosphorylation
T1145 Phosphorylation
T1148 Phosphorylation
S1151 Phosphorylation
S1154 Phosphorylation
S1164 Phosphorylation
T1169 Phosphorylation
K1184 Ubiquitination
S1195 Phosphorylation
S1196 Phosphorylation
S1206 Phosphorylation
K1207 Sumoylation
K1207 Ubiquitination
Y1212 Phosphorylation
K1215 Sumoylation
S1221 Phosphorylation
Y1222 Phosphorylation
K1226 Sumoylation
K1226 Ubiquitination
S1242 Phosphorylation
K1244 Ubiquitination
S1246 Phosphorylation
Y1247 Phosphorylation
S1249 Phosphorylation
S1259 Phosphorylation
S1263 Phosphorylation
S1281 Phosphorylation
S1284 Phosphorylation
K1287 Ubiquitination
S1293 Phosphorylation
T1300 Phosphorylation
S1322 Phosphorylation
T1333 Phosphorylation
K1336 Acetylation
S1350 Phosphorylation
T1367 Phosphorylation
T1386 Phosphorylation
K1389 Sumoylation
S1397 Phosphorylation
K1400 Sumoylation
K1400 Ubiquitination
K1404 Ubiquitination
S1405 Phosphorylation
T1408 Phosphorylation
S1411 Phosphorylation
K1412 Acetylation
K1412 Sumoylation
K1412 Ubiquitination
S1414 Phosphorylation
K1434 Sumoylation
Y1435 Phosphorylation
K1439 Sumoylation
T1443 Phosphorylation
T1449 Phosphorylation
S1450 Phosphorylation P31749 (AKT1)
S1453 Phosphorylation
S1457 Phosphorylation
S1472 Phosphorylation
Y1476 Phosphorylation
T1479 Phosphorylation
T1484 Phosphorylation
S1487 O-Glycosylation
Y1488 Phosphorylation
S1493 Phosphorylation
S1496 Phosphorylation
K1511 Ubiquitination
K1518 Methylation
K1518 Sumoylation
T1522 Phosphorylation
S1528 Phosphorylation
S1533 Phosphorylation
S1543 Phosphorylation
S1545 Phosphorylation
Y1560 Phosphorylation
R1561 Methylation
R1576 Methylation
R1589 Methylation
S1592 Phosphorylation
Y1597 Phosphorylation
R1628 Methylation
R1633 Methylation
S1636 Phosphorylation
R1638 Methylation
R1650 Methylation
T1670 Phosphorylation
S1671 Phosphorylation
T1672 Phosphorylation
S1742 Phosphorylation
S1750 Phosphorylation
Y1753 Phosphorylation
S1756 Phosphorylation
S1758 Phosphorylation
T1766 Phosphorylation
T1785 Phosphorylation
S1806 Phosphorylation
K1869 Sumoylation
K1869 Ubiquitination
K1875 Ubiquitination
T1925 Phosphorylation
K1945 Acetylation
S1952 Phosphorylation
S1955 Phosphorylation
S1957 Phosphorylation
S1959 Phosphorylation
S1960 Phosphorylation
S1963 Phosphorylation
S1977 Phosphorylation
S1980 Phosphorylation
S1981 Phosphorylation
T1991 Phosphorylation
Y1992 Phosphorylation
K1998 Sumoylation
K1998 Ubiquitination
T2050 Phosphorylation
S2084 Phosphorylation
S2086 Phosphorylation
S2090 Phosphorylation
T2091 Phosphorylation
S2102 Phosphorylation
S2105 Phosphorylation
S2117 Phosphorylation
S2120 Phosphorylation
S2136 Phosphorylation
Y2147 Phosphorylation
S2151 Phosphorylation
S2169 Phosphorylation
S2184 Phosphorylation
S2187 Phosphorylation
K2197 Sumoylation
T2201 Phosphorylation
S2202 Phosphorylation
S2218 Phosphorylation
S2219 Phosphorylation
S2224 Phosphorylation
S2253 Phosphorylation
S2259 Phosphorylation
S2274 Phosphorylation
S2315 Phosphorylation
S2318 Phosphorylation
S2335 Phosphorylation
S2337 Phosphorylation
T2347 Phosphorylation
S2352 Phosphorylation
S2354 Phosphorylation
S2355 Phosphorylation
S2358 Phosphorylation
S2394 Phosphorylation
S2395 Phosphorylation
T2396 Phosphorylation
T2399 Phosphorylation
Y2432 Phosphorylation
T2434 Phosphorylation
S2436 Phosphorylation P68400 (CSNK2A1)
S2438 Phosphorylation

研究背景

功能:

Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation. Mediates the NR1D1-dependent repression and circadian regulation of TSHB expression (By similarity). The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene ARTNL/BMAL1 and the genes involved in lipid metabolism in the liver (By similarity).

翻译修饰:

Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with BCL6, C1D, DACH1, HEXIM1, HDAC7, RORA, RORC, SAP30, SIAH2, SIN3A and SIN3B. May interact with DEAF1. Interacts with RXRA. Interacts with SETD5 (By similarity). Interacts with VDR. Interacts with ZBTB7A. Interacts with AR. Interacts with HDAC3 (By similarity).

蛋白家族:

The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).

The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity (By similarity).

Belongs to the N-CoR nuclear receptor corepressors family.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). DNA Methylation-Mediated GPX4 Transcriptional Repression and Osteoblast Ferroptosis Promote Titanium Particle-Induced Osteolysis. Research (Washington, D.C.), 2024 (PubMed: 39161535) [IF=8.5]

Application: WB    Species: Mouse    Sample:

Fig. 3. DNMT aberration-induced GPX4 suppression is co-regulated by KLF5. (A) Schematic diagram depicting mouse Gpx4 promoter luciferase reporter, Gpx4p-luc, the KLF5 binding motif, and its position relative to the transcription starting site. (B) Western blot. Calvarial tissues treated with control and TP (20 mg per mouse, 2 weeks) were assayed for KLF5, NCoR, SnoN, and SMRT, with β-actin serving as control. Two random samples per group were shown. Quantification beside blots was presented as mean ± SEM; n = 6; *P < 0.05, Student’s t test. (C) ChIP. Sham or TP-treated calvaria treated with or without SGI-1027 (2.5 mg/kg daily) were immunoprecipitated first with antibodies to KLF5, SMRT, NCoR, or SnoN, and then the immunoprecipitated DNA fragments were amplified through PCR with primer set specific for the KLF5 motif-containing region of the Gpx4 promoter, respectively. The non-immunoprecipitated DNA served as control (Input). PCR products were resolved on agarose gels. Quantifications on the right side were presented as mean ± SEM; n = 6; *P < 0.05, 2-way ANOVA. (D) Western blot. Primary osteoblasts (OBs) treated without or with TP (100 μg/ml) and the KLF5 inhibitor ML264 (10 μM) for 48 h were assayed for GPX4, with β-actin serving as control. Quantification below was presented as mean ± SD of 3 replicated experiments. *P < 0.05, 2-way ANOVA. (E) Luciferase assay. Primary OBs were transfected with a mouse Gpx4 promoter-luciferase reporter (Gpx4p-luc) and a Renilla luciferase reporter control, and then treated without or with TP (100 μg/ml) and SGI-1027 (10 μM) in the absence or presence of ML264 (10 μM) for 48 h. Luciferase activities of the Gpx4 promoter reporter were adjusted to Renilla luciferase activities and presented as box-and-whisker plots of 4 independent experiments. *P < 0.05, 3-way ANOVA.
2). Microglia nuclear receptor corepressor 1 deficiency alleviates neuroinflammation in mice. Neuroscience letters, 2024 (PubMed: 38242347) [IF=2.5]

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