产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0336, RRID:AB_2834176.
展开/折叠
Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; DRIP150; EXLM1; hRGR1; MED14; MED14_HUMAN; Mediator complex subunit 14; Mediator of RNA polymerase II transcription subunit 14; RGR1; RGR1 homolog; Thyroid hormone receptor-associated protein complex 170 kDa component; Transcriptional coactivator CRSP150; Trap170; Vitamin D3 receptor-interacting protein complex 150 kDa component;
抗原和靶标
- O60244 MED14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPVQLENHQLVPPGGGGGGSGGPPSAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60244 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K111 | Ubiquitination | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K399 | Methylation | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| K691 | Ubiquitination | Uniprot | |
| K699 | Ubiquitination | Uniprot | |
| Y901 | Phosphorylation | Uniprot | |
| Y927 | Phosphorylation | Uniprot | |
| Y941 | Phosphorylation | Uniprot | |
| K948 | Ubiquitination | Uniprot | |
| S977 | Phosphorylation | Uniprot | |
| S986 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S1061 | Phosphorylation | Uniprot | |
| S1112 | Phosphorylation | Uniprot | |
| S1116 | Phosphorylation | Uniprot | |
| S1119 | Phosphorylation | Uniprot | |
| R1123 | Methylation | Uniprot | |
| S1128 | Phosphorylation | Uniprot | |
| S1133 | Phosphorylation | Uniprot | |
| S1136 | Phosphorylation | Uniprot | |
| S1142 | Phosphorylation | Uniprot | |
| S1144 | Phosphorylation | Uniprot | |
| R1146 | Methylation | Uniprot | |
| S1151 | Phosphorylation | Uniprot | |
| K1256 | Ubiquitination | Uniprot | |
| K1264 | Ubiquitination | Uniprot | |
| K1274 | Ubiquitination | Uniprot | |
| K1283 | Ubiquitination | Uniprot | |
| K1295 | Ubiquitination | Uniprot | |
| K1304 | Ubiquitination | Uniprot | |
| T1354 | Phosphorylation | Uniprot | |
| K1360 | Ubiquitination | Uniprot | |
| S1361 | Phosphorylation | Uniprot | |
| K1420 | Ubiquitination | Uniprot |
研究背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Nucleus.
Ubiquitous.
Interacts with GATA1 (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR, ESR1, SREBF1 and STAT2.
Belongs to the Mediator complex subunit 14 family.
研究领域
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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