MAST3 Antibody - #AF0497

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产品: MAST3 抗体
货号: AF0497
描述: Rabbit polyclonal antibody to MAST3
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Dog
分子量: 143kDa; 143kD(Calculated).
蛋白号: O60307
RRID: AB_2834177

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
MAST3 Antibody detects endogenous levels of total MAST3.
RRID:
AB_2834177
引用格式: Affinity Biosciences Cat# AF0497, RRID:AB_2834177.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.7.11.1; KIAA0561; MAST 3; MAST3; MAST3_HUMAN; Microtubule associated serine/threonine protein kinase 3; Microtubule-associated serine/threonine-protein kinase 3;

抗原和靶标

免疫原:
Uniprot:

O60307(MAST3_HUMAN) >>Visit HPA database.

基因/基因ID:
MAST3(23031)
描述:
MAST3 Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Interacts with PTEN. Note: This description may include information from UniProtKB.
序列:
MDESSLLRRRGLQKELSLPRRGRGCRSGNRKSLVVGTPSPTLSRPLSPLSVPTAGSSPLDSPRNFSAASALNFPFARRADGRRWSLASLPSSGYGTNTPSSTLSSSSSSRERLHQLPFQPTPDELHFLSKHFRSSENVLDEEGGRSPRLRPRSRSLSPGRATGTFDNEIVMMNHVYRERFPKATAQMEGRLQEFLTAYAPGARLALADGVLGFIHHQIVELARDCLAKSGENLVTSRYFLEMQEKLERLLQDAHERSDSEEVSFIVQLVRKLLIIISRPARLLECLEFDPEEFYHLLEAAEGHAREGQGIKTDLPQYIIGQLGLAKDPLEEMVPLSHLEEEQPPAPESPESRALVGQSRRKPCESDFETIKLISNGAYGAVYLVRHRDTRQRFAIKKINKQNLILRNQIQQVFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVDMARLYFAETVLALEYLHNYGIVHRDLKPDNLLITSLGHIKLTDFGLSKIGLMSMATNLYEGHIEKDAREFIDKQVCGTPEYIAPEVIFRQGYGKPVDWWAMGVVLYEFLVGCVPFFGDTPEELFGQVVSDEIMWPEGDEALPADAQDLITRLLRQSPLDRLGTGGTHEVKQHPFFLALDWAGLLRHKAEFVPQLEAEDDTSYFDTRSERYRHLGSEDDETNDEESSTEIPQFSSCSHRFSKVYSSSEFLAVQPTPTFAERSFSEDREEGWERSEVDYGRRLSADIRLRSWTSSGSSCQSSSSQPERGPSPSLLNTISLDTMPKFAFSSEDEGVGPGPAGPKRPVFILGEPDPPPAATPVMPKPSSLSADTAALSHARLRSNSIGARHSTPRPLDAGRGRRLGGPRDPAPEKSRASSSGGSGGGSGGRVPKSASVSALSLIITADDGSGGPLMSPLSPRSLSSNPSSRDSSPSRDPSPVCGSLRPPIVIHSSGKKYGFSLRAIRVYMGDSDVYTVHHVVWSVEDGSPAQEAGLRAGDLITHINGESVLGLVHMDVVELLLKSGNKISLRTTALENTSIKVGPARKNVAKGRMARRSKRSRRRETQDRRKSLFKKISKQTSVLHTSRSFSSGLHHSLSSSESLPGSPTHSLSPSPTTPCRSPAPDVPADTTASPPSASPSSSSPASPAAAGHTRPSSLHGLAAKLGPPRPKTGRRKSTSSIPPSPLACPPISAPPPRSPSPLPGHPPAPARSPRLRRGQSADKLGTGERLDGEAGRRTRGPEAELVVMRRLHLSERRDSFKKQEAVQEVSFDEPQEEATGLPTSVPQIAVEGEEAVPVALGPTGRD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
73
Horse
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60307 作为底物

Site PTM Type Enzyme
T37 Phosphorylation
S39 Phosphorylation
S47 Phosphorylation
S66 Phosphorylation
S85 Phosphorylation
S88 Phosphorylation
T121 Phosphorylation
S134 Phosphorylation
S135 Phosphorylation
S146 Phosphorylation
S153 Phosphorylation
S155 Phosphorylation
S157 Phosphorylation
T164 Phosphorylation
Y176 Phosphorylation
S259 Phosphorylation
S277 Phosphorylation
S348 Phosphorylation
K361 Ubiquitination
T369 Phosphorylation
K400 Ubiquitination
S512 Phosphorylation
Y524 Phosphorylation
K530 Ubiquitination
K538 Ubiquitination
T665 Phosphorylation
S672 Phosphorylation
S680 Phosphorylation
T685 Phosphorylation
S698 Phosphorylation
S699 Phosphorylation
Y708 Phosphorylation
S709 Phosphorylation
S710 Phosphorylation
S711 Phosphorylation
S726 Phosphorylation
S728 Phosphorylation
S747 Phosphorylation
S754 Phosphorylation
S760 Phosphorylation
S774 Phosphorylation
S782 Phosphorylation
S792 Phosphorylation
S793 Phosphorylation
S847 Phosphorylation
R878 Methylation
S880 Phosphorylation
S885 Phosphorylation
S889 Phosphorylation
R892 Methylation
S935 Phosphorylation
S941 Phosphorylation
S963 Phosphorylation
Y977 Phosphorylation
S1060 Phosphorylation
S1063 Phosphorylation
K1081 Ubiquitination
S1084 Phosphorylation
S1091 Phosphorylation
S1102 Phosphorylation
S1136 Phosphorylation
S1201 Phosphorylation
S1203 Phosphorylation
S1215 Phosphorylation
S1223 Phosphorylation
S1257 Phosphorylation
S1262 Phosphorylation
S1273 Phosphorylation

研究背景

亚基结构:

Interacts with PTEN.

蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

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