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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0271, RRID:AB_2833352.
展开/折叠
FLJ43224; MGC104393; MGC125896; MGC125897; NED8; NEDD 8; NEDD-8; Nedd8; NEDD8_HUMAN; Neddylin; Neural precursor cell expressed developmentally down regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Neural precursor cell expressed developmentally down-regulated protein 8; Rub1; Ubiquitin like protein Nedd 8; Ubiquitin like protein Nedd8; Ubiquitin-like protein Nedd8;
抗原和靶标
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
- Q15843 NEDD8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15843 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| T7 | Phosphorylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| K11 | Acetylation | Uniprot | |
| K11 | Sumoylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| T20 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Sumoylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| K54 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y59 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S65 | Phosphorylation | Uniprot |
研究背景
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
Nucleus.
Note: Mainly nuclear.
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.
Belongs to the ubiquitin family.
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