| 产品: | FIR 抗体 |
| 货号: | AF0527 |
| 描述: | Rabbit polyclonal antibody to FIR |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Horse, Sheep, Dog |
| 分子量: | 119kDa; 120kD(Calculated). |
| 蛋白号: | O94887 |
| RRID: | AB_2834192 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0527, RRID:AB_2834192.
展开/折叠
FARP2; FARP2_HUMAN; FERM; FERM domain including RhoGEF; FERM, RhoGEF and pleckstrin domain protein 2; FERM, RhoGEF and pleckstrin domain-containing protein 2; FGD1 related Cdc42 GEF; FIR; FRG; KIAA0793; PH domain-containing family C member 3; Pleckstrin homology domain-containing family C member 3; PLEKHC3; RhoGEF and pleckstrin domain protein 2; RhoGEF and pleckstrin domain-containing protein 2;
抗原和靶标
- O94887 FARP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEIEGTYRVLQTAGMRLGAQTPVGVSTLEPGQTLLPRMQEKHLHLRVKLLDNTMEIFDIEPKCDGQVLLTQVWKRLNLVECDYFGMEFQNTQSYWIWLEPMKPIIRQIRRPKNVVLRLAVKFFPPDPGQLQEEYTRYLFALQLKRDLLEERLTCADTTAALLTSHLLQSEIGDYDETLDREHLKVNEYLPGQQHCLEKILEFHQKHVGQTPAESDFQVLEIARKLEMYGIRFHMASDREGTKIQLAVSHMGVLVFQGTTKINTFNWSKVRKLSFKRKRFLIKLHPEVHGPYQDTLEFLLGSRDECKNFWKICVEYHTFFRLLDQPKPKAKAVFFSRGSSFRYSGRTQKQLVDYFKDSGMKRIPYERRHSKTHTSVRALTADLPKQSISFPEGLRTPASPSSANAFYSLSPSTLVPSGLPEFKDSSSSLTDPQVSYVKSPAAERRSGAVAGGPDTPSAQPLGPPALQPGPGLSTKSPQPSPSSRKSPLSLSPAFQVPLGPAEQGSSPLLSPVLSDAGGAGMDCEEPRHKRVPADEAYFIVKEILATERTYLKDLEVITVWFRSAVVKEDAMPATLMTLLFSNIDPIYEFHRGFLREVEQRLALWEGPSKAHTKGSHQRIGDILLRNMRQLKEFTSYFQRHDEVLTELEKATKRCKKLEAVYKEFELQKVCYLPLNTFLLKPIQRLLHYRLLLRRLCGHYSPGHHDYADCHDALKAITEVTTTLQHILIRLENLQKLTELQRDLVGIENLIAPGREFIREGCLHKLTKKGLQQRMFFLFSDMLLYTSKGVAGTSHFRIRGLLPLQGMLVEESDNEWSVPHCFTIYAAQKTIVVAASTRLEKEKWMLDLNSAIQAAKSGGDTAPALPGRTVCTRPPRSPNEVSLEQESEDDARGVRSSLEGHGQHRANTTMHVCWYRNTSVSRADHSAAVENQLSGYLLRKFKNSHGWQKLWVVFTNFCLFFYKTHQDDYPLASLPLLGYSVSIPREADGIHKDYVFKLQFKSHVYFFRAESKYTFERWMEVIQGASSSAGRAPSIVQDGPQPSSGLEGMVRGKEE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O94887 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y8 | Phosphorylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| R337 | Methylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| R342 | Methylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| Y365 | Phosphorylation | Uniprot | |
| T380 | Phosphorylation | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| Y407 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| T413 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| T430 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| Y436 | Phosphorylation | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| T474 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| Y537 | Phosphorylation | Uniprot | |
| T574 | Phosphorylation | Uniprot | |
| T577 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| T645 | Phosphorylation | Uniprot | |
| T868 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| S886 | Phosphorylation | Uniprot | |
| T907 | Phosphorylation | Uniprot | |
| T908 | Phosphorylation | Uniprot | |
| Y914 | Phosphorylation | Uniprot | |
| S1033 | Phosphorylation | Uniprot |
研究背景
Functions as guanine nucleotide exchange factor that activates RAC1. May have relatively low activity. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Plays a role in TNFSF11-mediated osteoclast differentiation, especially in podosome rearrangement and reorganization of the actin cytoskeleton. Regulates the activation of ITGB3, integrin signaling and cell adhesion (By similarity).
Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding. Interacts with RAC1 (By similarity).
Intramolecular interaction between the DH domain and the PH domains can stabilize the protein in an autoinhibited conformation.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
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