产品: TRIM16 抗体
货号: AF0352
描述: Rabbit polyclonal antibody to TRIM16
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 63kDa; 64kD(Calculated).
蛋白号: O95361
RRID: AB_2834197

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
TRIM16 Antibody detects endogenous levels of total TRIM16.
RRID:
AB_2834197
引用格式: Affinity Biosciences Cat# AF0352, RRID:AB_2834197.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EBBP; Estrogen responsive B box protein; Estrogen-responsive B box protein; TRI16_HUMAN; TRIM 16; TRIM16; Tripartite motif containing 16; Tripartite motif containing protein 16; Tripartite motif-containing protein 16;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O95361 TRI16_HUMAN:

Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.

描述:
May play a role in the regulation of keratinocyte differentiation
序列:
MAELDLMAPGPLPRATAQPPAPLSPDSGSPSPDSGSASPVEEEDVGSSEKLGRETEEQDSDSAEQGDPAGEGKEVLCDFCLDDTRRVKAVKSCLTCMVNYCEEHLQPHQVNIKLQSHLLTEPVKDHNWRYCPAHHSPLSAFCCPDQQCICQDCCQEHSGHTIVSLDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP

翻译修饰 - O95361 作为底物

Site PTM Type Enzyme
S24 Phosphorylation
S29 Phosphorylation
S31 Phosphorylation
S38 Phosphorylation
K50 Ubiquitination
T55 Phosphorylation
S60 Phosphorylation
S62 Phosphorylation
S116 Phosphorylation
K172 Ubiquitination
K188 Methylation
K188 Ubiquitination
K202 Ubiquitination
S203 Phosphorylation
K240 Ubiquitination
K252 Ubiquitination
K264 Acetylation
K266 Acetylation
K289 Ubiquitination
K304 Ubiquitination
K306 Ubiquitination
K329 Ubiquitination
K331 Ubiquitination
K337 Ubiquitination
Y341 Phosphorylation
K354 Ubiquitination
K359 Ubiquitination
K384 Ubiquitination
Y429 Phosphorylation
Y430 Phosphorylation
Y441 Phosphorylation
Y531 Phosphorylation
S537 Phosphorylation
K554 Ubiquitination

研究背景

功能:

E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.

翻译修饰:

Phosphorylated by ULK1.

Auto-ubiquitinates via its B-Boxes.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.

亚基结构:

Homodimerizes via its coiled-coil domain. Heterodimerizes with MID1, TRIM24 and PML. Interacts with Galectin-3/LGALS3 in a ULK1-dependent manner; this interaction mediates autophagy of damage endomembranes. Interacts with BECN1. Interacts with ATG16L1. Interacts with p62/SQSTM and LC3B/MAP1LC3B.

蛋白家族:

Belongs to the TRIM/RBCC family.

文献引用

1). TRIM16 mitigates impaired osteogenic differentiation and antioxidant response in D-galactose-induced senescent osteoblasts. European journal of pharmacology, 2024 (PubMed: 39059569) [IF=5.0]

2). TFEB ameliorates DEHP-induced neurotoxicity by activating GAL3/TRIM16 axis dependent lysophagy and alleviating lysosomal dysfunction. Environmental toxicology, 2024 (PubMed: 38488668) [IF=4.5]

3). Tripartite motif-containing protein 16 protects against myocardial ischemia/reperfusion injury by affecting the Keap1/Nrf2 axis. CELL AND TISSUE RESEARCH, 2021 (PubMed: 34436665) [IF=3.6]

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