产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0461, RRID:AB_2834199.
展开/折叠
Apoptosis signal regulating kinase 2; Apoptosis signal-regulating kinase 2; ASK2; EC 2.7.11.25; M3K6_HUMAN; MAP3K6; MAPKKK6; MEKK6; MGC125653; MGC20114; Mitogen-activated protein kinase kinase kinase 6;
抗原和靶标
- O95382 M3K6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGPCPRSGAERAGSCWQDPLAVALSRGRQLAAPPGRGCARSRPLSVVYVLTREPQPGLEPREGTEAEPLPLRCLREACAQVPRPRPPPQLRSLPFGTLELGDTAALDAFYNADVVVLEVSSSLVQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDVFQKNSDCVGSYTLIPYVVTATGRVLCGDAGLLRGLADGLVQAGVGTEALLTPLVGRLARLLEATPTDSCGYFRETIRRDIRQARERFSGPQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDRAKALSVLLPLVQLEGSVAPDLYCMCGRIYKDMFFSSGFQDAGHREQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSKELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDPTQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPPGGPPRRAHFWLHFLLQSCQPFKTACAQGDQCLVLVLEMNKVLLPAKLEVRGTDPVSTVTLSLLEPETQDIPSSWTFPVASICGVSASKRDERCCFLYALPPAQDVQLCFPSVGHCQWFCGLIQAWVTNPDSTAPAEEAEGAGEMLEFDYEYTETGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHRRLRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGLGYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGSPQAAMFQVGMYKVHPPMPSSLSAEAQAFLLRTFEPDPRLRASAQTLLGDPFLQPGKRSRSPSSPRHAPRPSDAPSASPTPSANSTTQSQTFPCPQAPSQHPPSPPKRCLSYGGTSQLRVPEEPAAEEPASPEESSGLSLLHQESKRRAMLAAVLEQELPALAENLHQEQKQEQGARLGRNHVEELLRCLGAHIHTPNRRQLAQELRALQGRLRAQGLGPALLHRPLFAFPDAVKQILRKRQIRPHWMFVLDSLLSRAVRAALGVLGPEVEKEAVSPRSEELSNEGDSQQSPGQQSPLPVEPEQGPAPLMVQLSLLRAETDRLREILAGKEREYQALVQRALQRLNEEARTYVLAPEPPTALSTDQGLVQWLQELNVDSGTIQMLLNHSFTLHTLLTYATRDDLIYTRIRGGMVCRIWRAILAQRAGSTPVTSGP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95382 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S251 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| K677 | Ubiquitination | Uniprot | |
| Y717 | Phosphorylation | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| T801 | Phosphorylation | Uniprot | |
| T806 | Phosphorylation | O95382 (MAP3K6) | Uniprot |
| S896 | Phosphorylation | Uniprot | |
| T899 | Phosphorylation | Uniprot | |
| K910 | Ubiquitination | Uniprot | |
| S912 | Phosphorylation | Uniprot | |
| S917 | Phosphorylation | Uniprot | |
| S964 | Phosphorylation | Uniprot | |
| S984 | Phosphorylation | Uniprot | |
| K999 | Ubiquitination | Uniprot | |
| S1129 | Phosphorylation | Uniprot | |
| S1149 | Phosphorylation | Uniprot | |
| S1281 | Phosphorylation | Uniprot |
翻译修饰 - O95382 作为激酶
研究背景
Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways.
Binds both upstream activators and downstream substrates in multimolecular complexes.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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