产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0512, RRID:AB_2834205.
展开/折叠
FLH21957; FLJ10410; FLJ20373; FLJ90111; Hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; HPK/GCK like kinase HGK; HPK/GCK-like kinase HGK; KIAA0687; M4K4_HUMAN; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen activated protein kinase kinase kinase kinase 4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck interacting kinase; Nck-interacting kinase; NIK;
抗原和靶标
Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
- O95819 M4K4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95819 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
S5 | Phosphorylation | Uniprot | |
S17 | Phosphorylation | Uniprot | |
T35 | Phosphorylation | Uniprot | |
Y36 | Phosphorylation | Uniprot | |
K46 | Ubiquitination | Uniprot | |
K54 | Ubiquitination | Uniprot | |
T59 | Phosphorylation | Uniprot | |
Y86 | Phosphorylation | Uniprot | |
S93 | Phosphorylation | Uniprot | |
T124 | Phosphorylation | Uniprot | |
Y132 | Phosphorylation | Uniprot | |
S134 | Phosphorylation | Uniprot | |
K155 | Ubiquitination | Uniprot | |
S175 | Phosphorylation | Uniprot | |
T181 | Phosphorylation | Uniprot | |
T187 | Phosphorylation | Uniprot | |
T191 | Phosphorylation | Uniprot | |
Y193 | Phosphorylation | Uniprot | |
K261 | Ubiquitination | Uniprot | |
S264 | Phosphorylation | Uniprot | |
K285 | Ubiquitination | Uniprot | |
K303 | Ubiquitination | Uniprot | |
T319 | Phosphorylation | Uniprot | |
Y321 | Phosphorylation | Uniprot | |
Y323 | Phosphorylation | Uniprot | |
S324 | Phosphorylation | Uniprot | |
S326 | Phosphorylation | Uniprot | |
T351 | Phosphorylation | Uniprot | |
Y467 | Phosphorylation | Uniprot | |
Y520 | Phosphorylation | Uniprot | |
S523 | Phosphorylation | Uniprot | |
S536 | Phosphorylation | Uniprot | |
R546 | Methylation | Uniprot | |
S547 | Phosphorylation | Uniprot | |
K548 | Ubiquitination | Uniprot | |
S550 | Phosphorylation | Uniprot | |
Y560 | Phosphorylation | Uniprot | |
T576 | Phosphorylation | Uniprot | |
S580 | Phosphorylation | Uniprot | |
S598 | Phosphorylation | Uniprot | |
S600 | Phosphorylation | Uniprot | |
S602 | Phosphorylation | Uniprot | |
S604 | Phosphorylation | Uniprot | |
T627 | Phosphorylation | Uniprot | |
T628 | Phosphorylation | Uniprot | |
S629 | Phosphorylation | Uniprot | |
S631 | Phosphorylation | Uniprot | |
S639 | Phosphorylation | Uniprot | |
S644 | Phosphorylation | Uniprot | |
S649 | Phosphorylation | Uniprot | |
S656 | Phosphorylation | Uniprot | |
S677 | Phosphorylation | Uniprot | |
S679 | Phosphorylation | Uniprot | |
S680 | Phosphorylation | Uniprot | |
S681 | Phosphorylation | Uniprot | |
S683 | Phosphorylation | Uniprot | |
S684 | Phosphorylation | Uniprot | |
S686 | Phosphorylation | Uniprot | |
S688 | Phosphorylation | Uniprot | |
S692 | Phosphorylation | Uniprot | |
S696 | Phosphorylation | Uniprot | |
S698 | Phosphorylation | Uniprot | |
S700 | Phosphorylation | Uniprot | |
S708 | Phosphorylation | Uniprot | |
S709 | Phosphorylation | Uniprot | |
S710 | Phosphorylation | Uniprot | |
S712 | Phosphorylation | Uniprot | |
S715 | Phosphorylation | Uniprot | |
S717 | Phosphorylation | Uniprot | |
K738 | Ubiquitination | Uniprot | |
T743 | Phosphorylation | Uniprot | |
S791 | Phosphorylation | Uniprot | |
S800 | Phosphorylation | Uniprot | |
S801 | Phosphorylation | Uniprot | |
S805 | Phosphorylation | Uniprot | |
T809 | Phosphorylation | Uniprot | |
S811 | Phosphorylation | Uniprot | |
S823 | Phosphorylation | Uniprot | |
T828 | Phosphorylation | Uniprot | |
S830 | Phosphorylation | Uniprot | |
K831 | Ubiquitination | Uniprot | |
T834 | Phosphorylation | Uniprot | |
T840 | Phosphorylation | Uniprot | |
S842 | Phosphorylation | Uniprot | |
T846 | Phosphorylation | Uniprot | |
K849 | Methylation | Uniprot | |
K849 | Ubiquitination | Uniprot | |
K851 | Methylation | Uniprot | |
K851 | Ubiquitination | Uniprot | |
S852 | Phosphorylation | Uniprot | |
S853 | Phosphorylation | Uniprot | |
S854 | Phosphorylation | Uniprot | |
S855 | Phosphorylation | Uniprot | |
T857 | Phosphorylation | Uniprot | |
S868 | Phosphorylation | Uniprot | |
S870 | Phosphorylation | Uniprot | |
S871 | Phosphorylation | Uniprot | |
T873 | Phosphorylation | Uniprot | |
T874 | Phosphorylation | Uniprot | |
T876 | Phosphorylation | Uniprot | |
S877 | Phosphorylation | Uniprot | |
K898 | Ubiquitination | Uniprot | |
S900 | Phosphorylation | Uniprot | |
T907 | Phosphorylation | Uniprot | |
S913 | Phosphorylation | Uniprot | |
T915 | Phosphorylation | Uniprot | |
K957 | Ubiquitination | Uniprot | |
Y992 | Phosphorylation | Uniprot | |
S995 | Phosphorylation | Uniprot | |
K1010 | Ubiquitination | Uniprot | |
K1053 | Ubiquitination | Uniprot | |
K1057 | Ubiquitination | Uniprot | |
K1070 | Ubiquitination | Uniprot | |
Y1143 | Phosphorylation | Uniprot | |
T1146 | Phosphorylation | Uniprot | |
K1152 | Ubiquitination | Uniprot | |
K1179 | Ubiquitination | Uniprot | |
K1198 | Ubiquitination | Uniprot | |
K1204 | Ubiquitination | Uniprot | |
K1212 | Ubiquitination | Uniprot | |
S1224 | Phosphorylation | Uniprot | |
Y1227 | Phosphorylation | Uniprot | |
T1230 | Phosphorylation | Uniprot |
翻译修饰 - O95819 作为激酶
研究背景
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.
Cytoplasm.
Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Interacts (via its CNH regulatory domain) with ATL1 (via the N-terminal region). Interacts with RAP2A (GTP-bound form preferentially).
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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