产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0356, RRID:AB_2834208.
展开/折叠
Adenylate kinase 1; Adenylate kinase isoenzyme 1; Adenylate kinase soluble; AK 1; Ak1; ATP AMP transphosphorylase; ATP-AMP transphosphorylase 1; KAD1; KAD1_HUMAN; Myokinase;
抗原和靶标
- P00568 KAD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00568 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| C25 | S-Nitrosylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| R107 | Methylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| C187 | S-Nitrosylation | Uniprot |
研究背景
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
Cytoplasm.
Monomer.
Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.
Belongs to the adenylate kinase family. AK1 subfamily.
研究领域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Thiamine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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