| 产品: | AAK1 抗体 |
| 货号: | AF0685 |
| 描述: | Rabbit polyclonal antibody to AAK1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 94kDa; 104kD(Calculated). |
| 蛋白号: | Q2M2I8 |
| RRID: | AB_2834213 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0685, RRID:AB_2834213.
展开/折叠
AAK 1; Aak1; AAK1_HUMAN; Adaptor associated kinase 1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2 associated protein kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570;
抗原和靶标
Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
- Q2M2I8 AAK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPATQKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPVRQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLLDCSLLSNPTTDLLEEFAPTAISAPVHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q2M2I8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| T221 | Phosphorylation | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| K317 | Methylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| T347 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| T359 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | P06493 (CDK1) | Uniprot |
| R391 | Methylation | Uniprot | |
| T441 | Phosphorylation | Uniprot | |
| T605 | Phosphorylation | Uniprot | |
| T606 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| T620 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| T627 | Phosphorylation | Uniprot | |
| S637 | Phosphorylation | Q15208 (STK38) | Uniprot |
| T640 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| T653 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| T673 | Phosphorylation | Uniprot | |
| T674 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| T681 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| Y687 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| K705 | Acetylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| S797 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| T848 | Phosphorylation | Uniprot | |
| T852 | Phosphorylation | Uniprot | |
| S951 | Phosphorylation | Uniprot |
翻译修饰 - Q2M2I8 作为激酶
研究背景
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Autophosphorylated.
Cell membrane>Peripheral membrane protein. Membrane>Clathrin-coated pit. Cell junction>Synapse>Presynapse.
Note: Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.
Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
Interacts with alpha-adaptin, AP-2, clathrin, NUMB and EPS15 isoform 2. Interacts with membrane-bound activated NOTCH1 but not with the inactive full-length form of NOTCH1. Preferentially interacts with monoubiquitinated activated NOTCH1 compared to the non-ubiquitinated form.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
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