产品: DCP1A 抗体
货号: AF0551
描述: Rabbit polyclonal antibody to DCP1A
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 75kDa; 63kD(Calculated).
蛋白号: Q9NPI6
RRID: AB_2834222

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
DCP1A Antibody detects endogenous levels of total DCP1A.
RRID:
AB_2834222
引用格式: Affinity Biosciences Cat# AF0551, RRID:AB_2834222.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DCP1 decapping enzyme homolog A; Dcp1a; DCP1A_HUMAN; Decapping enzyme hDcp1a; Decapping mRNA 1A; HSA275986; mRNA decapping enzyme 1A; mRNA-decapping enzyme 1A; Nbla00360; Putative protein product of Nbla00360; Smad4 interacting transcriptional co activator; Smad4-interacting transcriptional co-activator; Smad4-interacting transcriptional co-activator; SMAD4IP1; SMIF; Transcription factor SMIF;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9NPI6 DCP1A_HUMAN:

Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.

描述:
Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas
序列:
MEALSRAGQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQLSNLGSTETLEEMPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHHSVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVLSPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPRQRSPLLNQPVPELSHASLIANQSPFRAPLNVTNTAGTSLPSVDLLQKLRLTPQHDQIQTQPLGKGAMVASFSPAAGQLATPESFIEPPSKTAAARVAASASLSNMVLAPLQSMQQNQDPEVFVQPKVLSSAIPVAGAPLVTATTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLTIGTPESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHEVYLQVLTKNKDNHNL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
90
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NPI6 作为底物

Site PTM Type Enzyme
S12 Phosphorylation
K47 Ubiquitination
S60 Phosphorylation
S62 Phosphorylation
Y64 Phosphorylation
K120 Ubiquitination
K140 Ubiquitination
S142 Phosphorylation
S144 Phosphorylation
S176 Phosphorylation
S179 Phosphorylation
S180 Phosphorylation
S186 Phosphorylation
T195 Phosphorylation
T197 Phosphorylation
K215 Ubiquitination
S226 Phosphorylation
K229 Ubiquitination
T290 Phosphorylation
T295 Phosphorylation
T309 Phosphorylation
Y310 Phosphorylation
T311 Phosphorylation
S315 Phosphorylation P45983 (MAPK8)
S319 Phosphorylation
T321 Phosphorylation
S334 Phosphorylation
T340 Phosphorylation
K346 Ubiquitination
T347 Phosphorylation
T348 Phosphorylation
S353 Phosphorylation
S373 Phosphorylation
R376 Methylation
K397 Ubiquitination
T401 Phosphorylation
S422 Phosphorylation
T430 Phosphorylation
S433 Phosphorylation
K520 Ubiquitination
S522 Phosphorylation
S523 Phosphorylation
S525 Phosphorylation
T528 Phosphorylation
T531 Phosphorylation
S534 Phosphorylation
K537 Ubiquitination
S539 Phosphorylation
S543 Phosphorylation
S545 Phosphorylation

研究背景

功能:

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.

细胞定位:

Cytoplasm>P-body. Nucleus.
Note: Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB) (PubMed:16364915). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (PubMed:11836524).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.

亚基结构:

Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3. Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1. Interacts with DCP1B. Interacts with DCP2. Interacts with DDX17 in an RNA-independent manner. Interacts with PNRC2. Interacts with SMAD4. Interacts with UPF1. Interacts with ZC3HAV1. Interacts with ZFP36L1. Interacts with NBDY.

蛋白家族:

Belongs to the DCP1 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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