| 产品: | MED17 抗体 |
| 货号: | AF0440 |
| 描述: | Rabbit polyclonal antibody to MED17 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 73kDa; 73kD(Calculated). |
| 蛋白号: | Q9NVC6 |
| RRID: | AB_2834223 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0440, RRID:AB_2834223.
展开/折叠
77-KD subunit; Activator recruited cofactor 77 kDa component; Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa; CRSP complex subunit 6; CRSP6; CRSP77; DRIP80; med17; MED17_HUMAN; Mediator complex subunit 17; Mediator of RNA polymerase II transcription subunit 17; Mediator of RNA polymerase II transcription subunit 17, S cerevisiae, homolog of; Thyroid hormone receptor-associated protein complex 80 kDa component; Thyroid hormone receptor-associated protein, 80-KD subunit; Transcriptional coactivator CRSP77; Trap80; VITAMIN D RECEPTOR-INTERACTING PROTEIN, 80-KD; Vitamin D3 receptor-interacting protein complex 80 kDa component;
抗原和靶标
- Q9NVC6 MED17_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGVRAVRISIESACEKQVHEVGLDGTETYLPPLSMSQNLARLAQRIDFSQGSGSEEEEAAGTEGDAQEWPGAGSSADQDDEEGVVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQNPQTLQLISKKKSLAGAAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDLDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NVC6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| Y256 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K278 | Acetylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K353 | Acetylation | Uniprot | |
| K356 | Acetylation | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K361 | Acetylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K436 | Ubiquitination | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| K606 | Ubiquitination | Uniprot | |
| K614 | Ubiquitination | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| Y637 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot |
研究背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Nucleus.
Ubiquitous.
Interacts with GATA1 and PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with STAT2.
Belongs to the Mediator complex subunit 17 family.
研究领域
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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