GAD1 Antibody - #AF0163

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产品: GAD1 抗体
货号: AF0163
描述: Rabbit polyclonal antibody to GAD1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 67kDa; 67kD(Calculated).
蛋白号: Q99259
RRID: AB_2833356

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
GAD1 Antibody detects endogenous levels of total GAD1.
RRID:
AB_2833356
引用格式: Affinity Biosciences Cat# AF0163, RRID:AB_2833356.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

67 kDa glutamic acid decarboxylase; CPSQ1; DCE1; DCE1_HUMAN; EC 4.1.1.15; FLJ45882; GAD 67; GAD; GAD-67; GAD1; Glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 1; Glutamate decarboxylase 1 brain 67kD; Glutamate decarboxylase 1 brain 67kDa; Glutamate decarboxylase 67 kDa isoform; Glutamate decarboxylase, brain, 67-KD; OTTHUMP00000041055; SCP;

抗原和靶标

免疫原:
Uniprot:

Q99259(DCE1_HUMAN) >>Visit HPA database.

基因/基因ID:
GAD1(2571)
表达:
Q99259 DCE1_HUMAN:

Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

描述:
GAD67 Catalyzes the production of GABA. Belongs to the group II decarboxylase family. Homodimer. 3 isoforms of the human protein are produced by alternative splicing.
序列:
MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIVGWSSKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEQSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADFEAKILEAKQKGYVPFYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLNGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPQRREKLHKVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99259 作为底物

Site PTM Type Enzyme
K60 Ubiquitination
K68 Ubiquitination
K74 Ubiquitination
T91 Phosphorylation P17612 (PRKACA)
Y265 Phosphorylation
S571 Phosphorylation
S578 Phosphorylation

研究背景

功能:

Catalyzes the production of GABA.

组织特异性:

Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the group II decarboxylase family.

研究领域

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.

· Metabolism > Metabolism of other amino acids > Taurine and hypotaurine metabolism.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > GABAergic synapse.

文献引用

1). Transplantation of NEP1-40 and NT-3 Gene-Co-Transduced Neural Stem Cells Improves Function and Neurogenesis after Spinal Cord Injury in a Rat Model. NEUROLOGY INDIA, 2022 (PubMed: 36412377) [IF=2.7]

Application: IHC    Species: Rat    Sample:

Figure 4: (a) Expression of ChAT at the SCI lesion in the six groups as determined by immunohistochemical staining (×200 original magnification). (b) OD intensity of ChAT expression in all groups. (c) Expression of GAD67 at the SCI lesion in the six groups according to immunohistochemical staining (×200 original magnification). (d) OD intensity of GAD67 expression in all groups. *P < 0.05 as compared with the selected group, **P < 0.01 as compared with the selected group

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