产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0713, RRID:AB_2834224.
展开/折叠
Arachidonic acid epoxygenase; CP2J2_HUMAN; CPJ2; CYP2J2; CYPIIJ2; Cytochrome P450 2J2; Cytochrome P450 family 2 subfamily J polypeptide 2; Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2; Cytochrome P450 subfamily IIJ polypeptide 2; Flavoprotein linked monooxygenase; Microsomal monooxygenase; OTTHUMP00000010550;
抗原和靶标
Highly expressed in heart, present at lower levels in liver, kidney and skeletal muscle (at protein level).
- P51589 CP2J2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLAAMGSLAAALWAVVHPRTLLLGTVAFLLAADFLKRRRPKNYPPGPWRLPFLGNFFLVDFEQSHLEVQLFVKKYGNLFSLELGDISAVLITGLPLIKEALIHMDQNFGNRPVTPMREHIFKKNGLIMSSGQAWKEQRRFTLTALRNFGLGKKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAVSNIICSITFGERFEYQDSWFQQLLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMIDKHRKDWNPAETRDFIDAYLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQPSTAARESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDTFNPDHFLENGQFKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKFTFRPPNNEKLSLKFRMGITISPVSHRLCAVPQV
翻译修饰 - P51589 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T20 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| Y281 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot |
研究背景
A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) in the cardiovascular system. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of PUFA. Converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EpETrE), likely playing a major role in the epoxidation of endogenous cardiac arachidonic acid pools. In endothelial cells, participates in eicosanoids metabolism by converting hydroperoxide species into hydroxy epoxy metabolites. In combination with 15-lipoxygenase metabolizes arachidonic acid and converts hydroperoxyicosatetraenoates (HpETEs) into hydroxy epoxy eicosatrienoates (HEETs), which are precursors of vasodilatory trihydroxyicosatrienoic acids (THETAs). This hydroperoxide isomerase activity is NADPH- and O2-independent. Catalyzes the monooxygenation of a various xenobiotics, such as danazol, amiodarone, terfenadine, astemizole, thioridazine, tamoxifen, cyclosporin A and nabumetone. Catalyzes hydroxylation of the anthelmintics albendazole and fenbendazole. Catalyzes the sulfoxidation of fenbedazole.
Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.
Highly expressed in heart, present at lower levels in liver, kidney and skeletal muscle (at protein level).
Belongs to the cytochrome P450 family.
研究领域
· Metabolism > Lipid metabolism > Arachidonic acid metabolism.
· Metabolism > Lipid metabolism > Linoleic acid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Ovarian steroidogenesis.
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