(2)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0410, RRID:AB_2834236.
展开/折叠
ATP7B; ATP7B_HUMAN; ATPase, Cu(2+) transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide; Copper pump 2; Copper transporting ATPase 2; PWD; Toxic milk; tx; WC1; WD; Wilson disease associated protein; Wilson disease-associated protein; WND; WND/140 kDa;
抗原和靶标
Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
- P35670 ATP7B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPEQERQITAREGASRKILSKLSLPTRAWEPAMKKSFAFDNVGYEGGLDGLGPSSQVATSTVRILGMTCQSCVKSIEDRISNLKGIISMKVSLEQGSATVKYVPSVVCLQQVCHQIGDMGFEASIAEGKAASWPSRSLPAQEAVVKLRVEGMTCQSCVSSIEGKVRKLQGVVRVKVSLSNQEAVITYQPYLIQPEDLRDHVNDMGFEAAIKSKVAPLSLGPIDIERLQSTNPKRPLSSANQNFNNSETLGHQGSHVVTLQLRIDGMHCKSCVLNIEENIGQLLGVQSIQVSLENKTAQVKYDPSCTSPVALQRAIEALPPGNFKVSLPDGAEGSGTDHRSSSSHSPGSPPRNQVQGTCSTTLIAIAGMTCASCVHSIEGMISQLEGVQQISVSLAEGTATVLYNPSVISPEELRAAIEDMGFEASVVSESCSTNPLGNHSAGNSMVQTTDGTPTSVQEVAPHTGRLPANHAPDILAKSPQSTRAVAPQKCFLQIKGMTCASCVSNIERNLQKEAGVLSVLVALMAGKAEIKYDPEVIQPLEIAQFIQDLGFEAAVMEDYAGSDGNIELTITGMTCASCVHNIESKLTRTNGITYASVALATSKALVKFDPEIIGPRDIIKIIEEIGFHASLAQRNPNAHHLDHKMEIKQWKKSFLCSLVFGIPVMALMIYMLIPSNEPHQSMVLDHNIIPGLSILNLIFFILCTFVQLLGGWYFYVQAYKSLRHRSANMDVLIVLATSIAYVYSLVILVVAVAEKAERSPVTFFDTPPMLFVFIALGRWLEHLAKSKTSEALAKLMSLQATEATVVTLGEDNLIIREEQVPMELVQRGDIVKVVPGGKFPVDGKVLEGNTMADESLITGEAMPVTKKPGSTVIAGSINAHGSVLIKATHVGNDTTLAQIVKLVEEAQMSKAPIQQLADRFSGYFVPFIIIMSTLTLVVWIVIGFIDFGVVQRYFPNPNKHISQTEVIIRFAFQTSITVLCIACPCSLGLATPTAVMVGTGVAAQNGILIKGGKPLEMAHKIKTVMFDKTGTITHGVPRVMRVLLLGDVATLPLRKVLAVVGTAEASSEHPLGVAVTKYCKEELGTETLGYCTDFQAVPGCGIGCKVSNVEGILAHSERPLSAPASHLNEAGSLPAEKDAVPQTFSVLIGNREWLRRNGLTISSDVSDAMTDHEMKGQTAILVAIDGVLCGMIAIADAVKQEAALAVHTLQSMGVDVVLITGDNRKTARAIATQVGINKVFAEVLPSHKVAKVQELQNKGKKVAMVGDGVNDSPALAQADMGVAIGTGTDVAIEAADVVLIRNDLLDVVASIHLSKRTVRRIRINLVLALIYNLVGIPIAAGVFMPIGIVLQPWMGSAAMAASSVSVVLSSLQLKCYKKPDLERYEAQAHGHMKPLTASQVSVHIGMDDRWRDSPRATPWDQVSYVSQVSLSSLTSDKPSRHSAAADDDGDKWSLLLNGRDEEQYI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P35670 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T9 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| K1028 | Acetylation | Uniprot | |
| K1028 | Ubiquitination | Uniprot | |
| T1050 | Phosphorylation | Uniprot | |
| T1076 | Phosphorylation | Uniprot | |
| Y1078 | Phosphorylation | Uniprot | |
| S1121 | Phosphorylation | Uniprot | |
| T1208 | Phosphorylation | Uniprot | |
| S1211 | Phosphorylation | Uniprot | |
| T1220 | Phosphorylation | Uniprot | |
| K1258 | Acetylation | Uniprot | |
| K1260 | Acetylation | Uniprot | |
| S1398 | Phosphorylation | Uniprot | |
| S1401 | Phosphorylation | Uniprot | |
| S1413 | Phosphorylation | Uniprot | |
| S1423 | Phosphorylation | Uniprot | |
| S1426 | Phosphorylation | Uniprot | |
| S1453 | Phosphorylation | Uniprot |
研究背景
Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.
Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein. Late endosome.
Note: Predominantly found in the trans-Golgi network (TGN). Localized in the trans-Golgi network under low copper conditions, redistributes to cytoplasmic vesicles when cells are exposed to elevated copper levels, and then recycles back to the trans-Golgi network when copper is removed (PubMed:10942420).
Golgi apparatus membrane>Multi-pass membrane protein.
Cytoplasm.
Mitochondrion.
Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
Monomer. Interacts with COMMD1/MURR1. Interacts with DCTN4, in a copper-dependent manner. Interacts with ATOX1. Interacts (via C-terminus) with ZBTB16/PLZF.
Each HMA domain can bind a copper ion, they are tightly packed and closely interact with each other. Wild-type ATP7B can usually be loaded with an average 5.5 copper atoms per molecule.
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
研究领域
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
文献引用
Application: WB Species: Human Sample: Caki-1 and OS-RC-2 cells
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