HNF4 alpha /gamma Antibody - #AF0534

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产品: HNF4 alpha /gamma 抗体
货号: AF0534
描述: Rabbit polyclonal antibody to HNF4 alpha /gamma
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 52kDa; 53kD,46kD(Calculated).
蛋白号: P41235 | Q14541
RRID: AB_2834237

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
HNF4 alpha /gamma Antibody detects endogenous levels of total HNF4 alpha /gamma.
RRID:
AB_2834237
引用格式: Affinity Biosciences Cat# AF0534, RRID:AB_2834237.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ39654; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4; Hepatocyte nuclear factor 4 gamma; Hepatocyte nuclear factor 4-gamma; HNF 4 gamma; HNF-4-gamma; Hnf4g; HNF4G_HUMAN; NR2A2; NR2A3; Nuclear receptor subfamily 2 group A member 2;

抗原和靶标

免疫原:
Uniprot:

P41235(HNF4A_HUMAN) >>Visit HPA database.

Q14541(HNF4G_HUMAN) >>Visit HPA database.

基因/基因ID:
HNF4A(3172) | HNF4G(3174)
表达:
Q14541 HNF4G_HUMAN:

Expressed in pancreas, kidney, small intestine and testis. Weakly expressed in colon. Not expressed in liver, skeletal muscle, lung, placenta, brain, heart, peripheral blood, ovary, prostate, thymus and spleen.

描述:
HNF4 alpha a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I.
序列:
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI

MNTTDNGVNCLCAICGDRATGKHYGASSCDGCKGFFRRSIRKSHVYSCRFSRQCVVDKDKRNQCRYCRLRKCFRAGMKKEAVQNERDRISTRRSTFDGSNIPSINTLAQAEVRSRQISVSSPGSSTDINVKKIASIGDVCESMKQQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMMYKDILLLGNNYVIHRNSCEVEISRVANRVLDELVRPFQEIQIDDNEYACLKAIVFFDPDAKGLSDPVKIKNMRFQVQIGLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFVKLFGMVKIDNLLQEMLLGGASNDGSHLHHPMHPHLSQDPLTGQTILLGPMSTLVHADQISTPETPLPSPPQGSGQEQYKIAANQASVISHQHLSKQKQL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
75
Zebrafish
75
Chicken
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P41235/Q14541 作为底物

Site PTM Type Enzyme
Y23 Phosphorylation P12931 (SRC)
Y72 Phosphorylation
S75 Phosphorylation
S76 Phosphorylation
S95 Phosphorylation
S99 Phosphorylation
K106 Acetylation
K108 Acetylation
K126 Acetylation
K127 Acetylation
S138 Phosphorylation
T139 Phosphorylation
S142 Phosphorylation P17612 (PRKACA)
S143 Phosphorylation P17612 (PRKACA)
Y144 Phosphorylation
S147 Phosphorylation
S148 Phosphorylation
S151 Phosphorylation
T166 Phosphorylation
S167 Phosphorylation Q16539 (MAPK14) , Q15759 (MAPK11)
S170 Phosphorylation
S190 Phosphorylation
K234 Ubiquitination
S262 Phosphorylation
S265 Phosphorylation
Y286 Phosphorylation P12931 (SRC)
Y288 Phosphorylation P12931 (SRC)
S303 Phosphorylation Q13131 (PRKAA1)
K307 Ubiquitination
K309 Ubiquitination
S313 Phosphorylation Q13131 (PRKAA1)
S318 Phosphorylation
S378 Phosphorylation
T429 Phosphorylation
T432 Phosphorylation
S436 Phosphorylation
S441 Phosphorylation
S443 Phosphorylation
T455 Phosphorylation
K458 Acetylation
S461 Phosphorylation
T467 Phosphorylation
T469 Phosphorylation
Site PTM Type Enzyme
Y24 Phosphorylation
S27 Phosphorylation
S28 Phosphorylation
K58 Acetylation
S118 Phosphorylation
S121 Phosphorylation
S124 Phosphorylation
T126 Phosphorylation

研究背景

功能:

Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.

翻译修饰:

Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.

Acetylation at Lys-458 lowers transcriptional activation by about two-fold.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with CLOCK, ARNTL, CRY1, CRY2, PER1 and PER2. Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionnally inactive. Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers.

蛋白家族:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the nuclear hormone receptor family. NR2 subfamily.

功能:

Transcription factor. Has a lower transcription activation potential than HNF4-alpha.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in pancreas, kidney, small intestine and testis. Weakly expressed in colon. Not expressed in liver, skeletal muscle, lung, placenta, brain, heart, peripheral blood, ovary, prostate, thymus and spleen.

蛋白家族:

Belongs to the nuclear hormone receptor family. NR2 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

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