产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0165, RRID:AB_2833358.
展开/折叠
80 kDa MCM3 associated protein; 80 kDa MCM3-associated protein; FLJ44336; FLJ45306; Germinal center associated nuclear protein; KIAA0572; MAP80; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; MCM3A; MCM3A_HUMAN; Mcm3ap; Minichromosome maintenance complex component 3 associated protein; Minichromosome maintenance deficient 3 associated protein; Minichromosome maintenance protein 3 associated protein; Protein GANP; SAC3;
抗原和靶标
Widely expressed (PubMed:11024281). Up-regulated in germinal center B-cells in tonsils (at protein level) (PubMed:11024281).
- O60318 GANP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNPTNPFSGQQPSAFSASSSNVGTLPSKPPFRFGQPSLFGQNSTLSGKSSGFSQVSSFPASSGVSHSSSVQTLGFTQTSSVGPFSGLEHTSTFVATSGPSSSSVLGNTGFSFKSPTSVGAFPSTSAFGQEAGEIVNSGFGKTEFSFKPLENAVFKPILGAESEPEKTQSQIASGFFTFSHPISSAPGGLAPFSFPQVTSSSATTSNFTFSKPVSSNNSLSAFTPALSNQNVEEEKRGPKSIFGSSNNSFSSFPVSSAVLGEPFQASKAGVRQGCEEAVSQVEPLPSLMKGLKRKEDQDRSPRRHGHEPAEDSDPLSRGDHPPDKRPVRLNRPRGGTLFGRTIQDVFKSNKEVGRLGNKEAKKETGFVESAESDHMAIPGGNQSVLAPSRIPGVNKEEETESREKKEDSLRGTPARQSNRSESTDSLGGLSPSEVTAIQCKNIPDYLNDRTILENHFGKIAKVQRIFTRRSKKLAVVHFFDHASAALARKKGKSLHKDMAIFWHRKKISPNKKPFSLKEKKPGDGEVSPSTEDAPFQHSPLGKAAGRTGASSLLNKSSPVKKPSLLKAHQFEGDSFDSASEGSEGLGPCVLSLSTLIGTVAETSKEKYRLLDQRDRIMRQARVKRTDLDKARTFVGTCLDMCPEKERYMRETRSQLSVFEVVPGTDQVDHAAAVKEYSRSSADQEEPLPHELRPLPVLSRTMDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTVSLIEKCTRFHIHCAHFMCEEPMSSFDAKINNENMTKCLQSLKEMYQDLRNKGVFCASEAEFQGYNVLLSLNKGDILREVQQFHPAVRNSSEVKFAVQAFAALNSNNFVRFFKLVQSASYLNACLLHCYFSQIRKDALRALNFAYTVSTQRSTIFPLDGVVRMLLFRDCEEATDFLTCHGLTVSDGCVELNRSAFLEPEGLSKTRKSVFITRKLTVSVGEIVNGGPLPPVPRHTPVCSFNSQNKYIGESLAAELPVSTQRPGSDTVGGGRGEECGVEPDAPLSSLPQSLPAPAPSPVPLPPVLALTPSVAPSLFQLSVQPEPPPPEPVPMYSDEDLAQVVDELIQEALQRDCEEVGSAGAAYAAAALGVSNAAMEDLLTAATTGILRHIAAEEVSKERERREQERQRAEEERLKQERELVLSELSQGLAVELMERVMMEFVRETCSQELKNAVETDQRVRVARCCEDVCAHLVDLFLVEEIFQTAKETLQELQCFCKYLQRWREAVTARKKLRRQMRAFPAAPCCVDVSDRLRALAPSAECPIAEENLARGLLDLGHAGRLGISCTRLRRLRNKTAHQMKVQHFYQQLLSDVAWASLDLPSLVAEHLPGRQEHVFWKLVLVLPDVEEQSPESCGRILANWLKVKFMGDEGSVDDTSSDAGGIQTLSLFNSLSSKGDQMISVNVCIKVAHGALSDGAIDAVETQKDLLGASGLMLLLPPKMKSEDMAEEDVYWLSALLQLKQLLQAKPFQPALPLVVLVPSPGGDAVEKEVEDGLMLQDLVSAKLISDYTVTEIPDTINDLQGSTKVLQAVQWLVSHCPHSLDLCCQTLIQYVEDGIGHEFSGRFFHDRRERRLGGLASQEPGAIIELFNSVLQFLASVVSSEQLCDLSWPVTEFAEAGGSRLLPHLHWNAPEHLAWLKQAVLGFQLPQMDLPPLGAPWLPVCSMVVQYASQIPSSRQTQPVLQSQVENLLHRTYCRWKSKSPSPVHGAGPSVMEIPWDDLIALCINHKLRDWTPPRLPVTSEALSEDGQICVYFFKNDLKKYDVPLSWEQARLQTQKELQLREGRLAIKPFHPSANNFPIPLLHMHRNWKRSTECAQEGRIPSTEDLMRGASAEELLAQCLSSSLLLEKEENKRFEDQLQQWLSEDSGAFTDLTSLPLYLPQTLVSLSHTIEPVMKTSVTTSPQSDMMREQLQLSEATGTCLGERLKHLERLIRSSREEEVASELHLSALLDMVDI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60318 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S46 | Phosphorylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| K347 | Methylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| R449 | Methylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| T530 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| K674 | Ubiquitination | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| K787 | Acetylation | Uniprot | |
| K787 | Ubiquitination | Uniprot | |
| Y864 | Phosphorylation | Uniprot | |
| Y873 | Phosphorylation | Uniprot | |
| K947 | Ubiquitination | Uniprot | |
| T955 | Phosphorylation | Uniprot | |
| S1101 | Phosphorylation | Uniprot | |
| Y1106 | Phosphorylation | Uniprot | |
| S1139 | Phosphorylation | Uniprot | |
| K1140 | Ubiquitination | Uniprot | |
| K1194 | Ubiquitination | Uniprot | |
| K1241 | Acetylation | Uniprot | |
| K1386 | Ubiquitination | Uniprot | |
| K1448 | Ubiquitination | Uniprot | |
| K1512 | Ubiquitination | Uniprot | |
| K1527 | Ubiquitination | Uniprot | |
| K1785 | Ubiquitination | Uniprot | |
| K1801 | Ubiquitination | Uniprot | |
| K1813 | Acetylation | Uniprot | |
| S1836 | Phosphorylation | Uniprot | |
| S1847 | Phosphorylation | Uniprot | |
| T1848 | Phosphorylation | Uniprot | |
| K1873 | Ubiquitination | Uniprot | |
| S1922 | Phosphorylation | Uniprot | |
| T1924 | Phosphorylation | Uniprot | |
| T1925 | Phosphorylation | Uniprot | |
| S1926 | Phosphorylation | Uniprot |
研究背景
As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.
Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.
Nucleus envelope. Nucleus>Nuclear pore complex. Nucleus>Nucleoplasm. Chromosome.
Note: Predominantly located at the nuclear envelope, facing the nucleus interior (PubMed:20005110, PubMed:21195085, PubMed:23591820). Localization at the nuclear pore complex requires NUP153, TPR and ALYREF/ALY (PubMed:23591820, PubMed:22307388). Also found associated with chromatin (PubMed:23652018). In B-cells, targeted to the immunoglobulin variable region genes (PubMed:23652018).
Cytoplasm. Nucleus.
Note: Translocates into the nucleus in the presence of MCM3 (PubMed:12226073). Associates with chromatin possibly through interaction with MCM3 (PubMed:12226073).
Widely expressed. Up-regulated in germinal center B-cells in tonsils (at protein level).
Isoform GANP: Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, 2 copies of ENY2, PCID2, SEM1/DSS1, and either centrin CETN2 or centrin CETN3. The TREX-2 complex also associates with ALYREF/ALY. Interacts with RNA polymerase II subunit POLR2A and with the transcription elongation factor SUPT5H/SPT5. Interacts (via FG-repeats) with NXF1; this interaction is not mediated by RNA. Isoform GANP interacts with nuclear envelope proteins NUP62, NUP153 and RANBP2/NUP358; interaction with NUP153 is required for full localization at the nuclear pore complex. Interacts with several RNA helicases, including DHX9, DDX21, and DDX39A/DDX39, and with DNA topoisomerase TOP2A. Directly interacts with AICDA/AID. Interacts with the glucocorticoid receptor NR3C1. Isoform MCM3AP: Interacts with the glucocorticoid receptor NR3C1. Interacts with MCM3; this interaction leads to MCM3 acetylation.
Belongs to the SAC3 family.
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