Phospho-HSL (Ser660) Antibody - #AF8026

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产品: 磷酸化 HSL (Ser660) 抗体
货号: AF8026
描述: Rabbit polyclonal antibody to Phospho-HSL (Ser660)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Dog
分子量: 81/83kDa; 117kD(Calculated).
蛋白号: Q05469
RRID: AB_2840089

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(88%), Horse(89%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-HSL (Ser660) Antibody detects endogenous levels of HSL only when phosphorylated at Ser660.
RRID:
AB_2840089
引用格式: Affinity Biosciences Cat# AF8026, RRID:AB_2840089.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; HSL; LHS; Lipase hormone sensitive; LIPE; LIPS_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q05469(LIPS_HUMAN) >>Visit HPA database.

基因/基因ID:
LIPE(3991)
序列:
MEPGSKSVSRSDWQPEPHQRPITPLEPGPEKTPIAQPESKTLQGSNTQQKPASNQRPLTQQETPAQHDAESQKEPRAQQKSASQEEFLAPQKPAPQQSPYIQRVLLTQQEAASQQGPGLGKESITQQEPALRQRHVAQPGPGPGEPPPAQQEAESTPAAQAKPGAKREPSAPTESTSQETPEQSDKQTTPVQGAKSKQGSLTELGFLTKLQELSIQRSALEWKALSEWVTDSESESDVGSSSDTDSPATMGGMVAQGVKLGFKGKSGYKVMSGYSGTSPHEKTSARNHRHYQDTASRLIHNMDLRTMTQSLVTLAEDNIAFFSSQGPGETAQRLSGVFAGVREQALGLEPALGRLLGVAHLFDLDPETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALVYYAQRLLVTNRPGVLFFEGDEGLTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTISIGLVSFGEHYKRNETGLSVAASSLFTSGRFAIDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASATVRVSRLLSLPPEAFEMPLTADPTLTVTISPPLAHTGPGPVLVRLISYDLREGQDSEELSSLIKSNGQRSLELWPRPQQAPRSRSLIVHFHGGGFVAQTSRSHEPYLKSWAQELGAPIISIDYSLAPEAPFPRALEECFFAYCWAIKHCALLGSTGERICLAGDSAGGNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Dog
100
Horse
89
Bovine
88
Rabbit
78
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q05469 作为底物

Site PTM Type Enzyme
T188 Phosphorylation
S196 Phosphorylation
S552 Phosphorylation
S565 Phosphorylation
K624 Ubiquitination
S630 Phosphorylation
S645 Phosphorylation
S649 Phosphorylation
S650 Phosphorylation
S660 Phosphorylation
Y741 Phosphorylation
T813 Phosphorylation
S825 Phosphorylation
S835 Phosphorylation
S853 Phosphorylation P17612 (PRKACA) , Q13976 (PRKG1)
S855 Phosphorylation Q14012 (CAMK1) , P17612 (PRKACA) , Q13131 (PRKAA1) , Q9UQM7 (CAMK2A)
K872 Ubiquitination
S950 Phosphorylation P17612 (PRKACA)
S951 Phosphorylation P17612 (PRKACA)
T955 Phosphorylation
T1015 Phosphorylation

研究背景

功能:

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

翻译修饰:

Phosphorylation by AMPK may block translocation to lipid droplets.

细胞定位:

Cell membrane. Membrane>Caveola. Cytoplasm>Cytosol.
Note: Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with CAVIN1 in the adipocyte cytoplasm. Interacts with PLIN5 (By similarity).

蛋白家族:

Belongs to the 'GDXG' lipolytic enzyme family.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

文献引用

1). Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis. Nature communications, 2024 (PubMed: 38615060) [IF=16.6]
2). Effect of salt promote the muscle triglyceride hydrolysis during dry-salting by inducing the phosphorylation of adipose tissue triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and lipid droplets splitting. Food Chemistry, 2020 (PubMed: 32454271) [IF=8.8]

Application: WB    Species: Pig    Sample: adipose

Fig. 3. Western blotting analysis of ATGL, HSL and their phosphorylated protein contents in adipose tissue during processing of dry-cured meat. (A) WB image of each dry-cured group. (B) ATGL relative protein level in adipose tissue. (C) p-ATGL(S406) relative protein level in adipose tissue. (D) HSL relative protein level in adipose tissue. (E) p-HSL(Ser563) relative protein level in adipose tissue. (F) p-HSL(Ser660) relative protein level in adipose tissue.
3). MIIP downregulation drives colorectal cancer progression through inducing peri-cancerous adipose tissue browning. Cell & bioscience, 2024 (PubMed: 38245780) [IF=7.5]
4). Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function. JOURNAL OF LIPID RESEARCH, 2019 (PubMed: 30552289) [IF=6.5]

Application: WB    Species: pig    Sample: porcine intramuscular preadipocytes

Fig.?5.|Melatonin-activated PKA and ERK1/2 mediate lipolysis in porcine intramuscular preadipocytes. A–J: Fully differentiated adipocytes were treated with control, 1 mM of melatonin, and 1 mM of melatonin plus 10 M of 4-P-PDOT for 24 h. The expression levels of PKA (A, B), ERK1/2 (C, D), HSL (E, F), PLIN1 (G, H), and ATGL (I, J) and the phosphorylation levels of PKA (p-PKA Thr197) (A, B), ERK1/2 (p-ERK1/2 Thr202/Tyr204) (C, D), HSL (pHSL Ser660) (E, F), PLIN1 (p-PLIN1 Ser522) (G, H), and -tubulin (I, J) were evaluated by Western blotting. The results are represented as the mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3).
5). Sesamol Alleviates Obesity-Related Hepatic Steatosis via Activating Hepatic PKA Pathway. Nutrients, 2020 (PubMed: 31991934) [IF=5.9]
6). Effective fraction from Simiao Wan prevents hepatic insulin resistant by inhibition of lipolysis via AMPK activation. Chinese Journal of Natural Medicines, 2022 (PubMed: 35369960) [IF=4.6]
7). Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism. Food & Nutrition Research, 2022 (PubMed: 35382382) [IF=3.3]

Application: WB    Species: mouse    Sample: skeletal muscle

Fig. 6. | Effects of sesamol intervention on the expressions of glucose and lipid metabolism-related proteins in skeletal muscle of the middle-aged mice.(e) Representative western blot images and quantification of (f) SREBP1-c, (g) p-HSL and HSL and (h) CPT1α. *P < 0.05,**P < 0.01.

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